Hoxa-10 deficient male mice exhibit abnormal development of the accessory sex organs

Podlasek, Carol A.; Seo, Robert; Clemens, J. Quentin; Ma, Liang; Maas, Richard L.; Bushman, Wade

doi:10.1002/(sici)1097-0177(199901)214:1<1::aid-dvdy1>3.0.co;2-2

Cited by 82 publications

(65 citation statements)

References 26 publications

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“…When the function of an isoform is lost, perhaps due to genetic manipulation or neuropathy following invasive surgery, another isoform can sometimes compensate for the lost function. 28,30,31 There are several NOS isoforms (-I, -II and -III) which have been identified in the penis. These isoforms are further subdivided by varying splice variants (-Ia and -Ib).…”

Section: Discussionmentioning

confidence: 99%

“…16,[28][29][30][31][32] Total RNA was isolated from Sprague-Dawley penises using the TRIzol (Gibco BRL, Grand Island, NY, USA) method, the RNA was DNAse treated with RQ1 DNAse (Roche, Indianapolis, IN, USA) to eliminate any genomic DNA contamination and was suspended in water.…”

Section: Rt-pcr (Reverse Transcriptase Polymerase Chain Reaction)mentioning

confidence: 99%

“…Non-competitive RT-PCR as a method of gene quantification has been utilized successfully in the past by members of our laboratory to quantify many different genes during embryogenesis and postnatal morphogenesis. 16,[28][29][30][31] This technique allows us to determine if gene expression is altered but does not yield the exact amount of starting RNA. We feel this technique is useful for our purposes.…”

Section: Quantitative Reverse Transcriptase Polymerase Chain Reactionmentioning

confidence: 99%

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Analysis of NOS isoform changes in a post radical prostatectomy model of erectile dysfunction

et al. 2001

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Optimal treatment of erectile dysfunction (ED) following radical prostatectomy remains a subject of much controversy and is a significant concern for prostate cancer patients requiring surgical intervention. Neural stimulation involving nitric oxide synthase (NOS) is a crucial aspect of the normal erection process. In this study NOS isoform interaction was evaluated to improve our understanding of molecular changes pertaining to erection post radical prostatectomy. Bilateral cavernous nerve (CN) resected and control adult male Sprague-Dawley rats were killed 7, 14 and 21 days after injury. RT-PCR, in situ hybridization, Western blot and immunohistochemical analysis were used to evaluate changes in NOS isoform expression and distribution. NOS-I protein was dramatically decreased after CN injury while NOS-III and NOS-II remained unchanged. A profound decrease in smooth muscle and endothelium was observed in the corpora. To our knowledge this is the first report of differential altered NOS isoform protein abundance under conditions which mimic radical prostatectomy. These results show the importance of maintaining at least partial innervation of the penis after surgical intervention and that endothelial and smooth muscle changes resulting from loss of innervation may account for the ED observed in prostatectomy patients.

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Section: Discussionmentioning

confidence: 99%

Section: Rt-pcr (Reverse Transcriptase Polymerase Chain Reaction)mentioning

confidence: 99%

Section: Quantitative Reverse Transcriptase Polymerase Chain Reactionmentioning

confidence: 99%

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Analysis of NOS isoform changes in a post radical prostatectomy model of erectile dysfunction

et al. 2001

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“…The cDNA bands were then quanti®ed through densitometry using the Kodak 1D Image Analysis Software (Rochester, NY). The methods of Siebert et al 14 and Podlasek et al 15 were used for quantitation of the gene products. The log of density vs cycle number was plotted for both glyceraldehyde-3-phosphate dehydrogenase (GAPDH) and PDE type 6.…”

Section: Quantitative Pcr Analysis Of Retinal Rnamentioning

confidence: 99%

SSI Prize Essay for Male Erectile Dysfunction—Research ‘Sildenafil causes a dose- and time-dependent downregulation of phosphodiesterase type 6 expression in the rat retina‘

Gonzalez¹,

Bervig²,

Podlasek³

et al. 1999

Int J Impot Res

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Objectives: Some authors have advocated the daily use of sildena®l for prophylaxis against, or treatment of, erectile dysfunction. However, no information has been published to support such a dosage regimen. The safety pro®le of uninterrupted use of sildena®l has not been evaluated as it pertains to alteration of PDE type 6 in the retina. In the present study we investigated whether short-or long-term exposure to a variety of sildena®l doses affect the expression of an enzyme important in the normal phototransduction cascade. Methods: Sustained-release sildena®l pellets were implanted in 120-day-old male rats with concentrations from 1 ± 200 mg. Rat retinal tissue was harvested 7, 14, and 29 days after implantation. Quantitative reverse transcriptase-polymerase chain reaction (RT-PCR) using GAPDH as an endogenous internal standard was used to quantitate PDE type 6 gene expression. Results: Expression of PDE type 6 was upregulated after 7 days with dosages 5 mg (P`0.02). Signi®cant downregulation of PDE type 6 expression was ®rst noted with high dose sildena®l 14 days after implantation (P`0.02). Expression of PDE type 6 was signi®cantly and profoundly downregulated 29 days after implantation for all pellet formulations ! !10 mg (P`0.01). Conclusions: Sildena®l downregulates PDE type 6 expression in a dose-and time-dependent fashion. These ®ndings support the explanation that PDE type 6 inhibition causes the dosedependent clinical effects of visual disturbance in men taking sildena®l. Implications for longterm, daily use of sildena®l in men are not clear.

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“…Furthermore, genetic studies using mutant mouse models have identified defects in morphogenesis (Bomgardner et al, 2003;Kitagaki et al, 2011;Podlasek et al, 1999), development (Robaire, 2005;Tomaszewski et al, 2007;Yoshio et al, 2010), and function (Grover et al, 2005;Krishnamurthy et al, 2001;Krishnamurthy et al, 2000;Ma et al, 2004;Mendive et al, 2006) of the epididymis as a result of deletions engineered in various gene loci.…”

Section: Introductionmentioning

confidence: 99%

Segment- And Cell-Specific Expression of Dtype Cyclins in the Postnatal Mouse Epididymis

Wang

2007

Biology of Reproduction

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Sperm transport, maturation and storage are the essential functions of the epididymis. The epididymis in the mouse is structurally characterized by regional and segmental organization including caput, corpus and cauda epididymis that are comprised of ten segments. Although several growth factor signaling pathways have been discovered in the epididymis, how these converge onto the cell cycle components is unknown. To begin to elucidate the growth factor control of cell cycle events in the epididymis, we analyzed the expression of D-type cyclins at different postnatal ages. At 7d, cyclin D1 was mainly expressed in the cauda epithelium, by 14d its expression occurred in the epithelium of caput, corpus and cauda that persisted up to 21d. By 42d, cyclin D1 was mostly detectable in the principal cells of the caput and corpus (segments 1-7) but not in the cauda epididymis. Expression of cyclin D2, unlike that of cyclin D1, was evident only at 42d but not earlier, and was mostly confined to corpus and cauda epithelium. In contrast to both cyclin D1 and D2, cyclin D3 was expressed primarily in the interstitium at 7d and by 21d its expression was localized to the epithelium of the corpus and cauda epididymis. By 42d, expression of cyclin D3 peaked in segments 6-10 and confined to basal and principal cells of the corpus and apical cells of the cauda epithelium. Ki67 immunoreactivity confirmed absence of cell proliferation despite continued expression of D-type cyclins in the adult epididymis. Collectively, on the basis of our immunophenotyping and protein expression data, we conclude that the D-type cyclins are expressed in a development -, segment-, and cell-specific manner in the postnatal mouse epididymis.

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Hoxa-10 deficient male mice exhibit abnormal development of the accessory sex organs

Cited by 82 publications

References 26 publications

Analysis of NOS isoform changes in a post radical prostatectomy model of erectile dysfunction

Analysis of NOS isoform changes in a post radical prostatectomy model of erectile dysfunction

SSI Prize Essay for Male Erectile Dysfunction—Research ‘Sildenafil causes a dose- and time-dependent downregulation of phosphodiesterase type 6 expression in the rat retina‘

Segment- And Cell-Specific Expression of Dtype Cyclins in the Postnatal Mouse Epididymis

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