HOXA10 Regulates p53 Expression and Matrigel Invasion in Human Breast Cancer Cells

Chu, Micheline C.; Selam, Belgin; Taylor, Hugh S.

doi:10.4161/cbt.3.6.848

Cited by 106 publications

(75 citation statements)

References 29 publications

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“…Hox gene expression is linked to apoptosis and angiogenesis. Hoxa5 and Hoxa10 can regulate p53 expression in human breast cancer cell lines (10,11). Induction of HOXA5 expression causes caspase 2-and caspase 8-mediated apoptotic cell death (32).…”

Section: Ncammentioning

confidence: 99%

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Identification of aHoxc8-regulated transcriptional network in mouse embryo fibroblast cells

Lei

Juan

Kim

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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The transcription factor, Hoxc8, is a member of the homeobox gene family that is vital for growth and differentiation. Previously, we identified 34 genes whose expression levels were changed at least 2-fold by forced expression of Hoxc8 in C57BL͞6J mouse embryo fibroblast cells using a mouse 16,463-gene oligonucleotide microarray. In the present study, we used the combined power of microarray profiling, global Hoxc8 DNA-binding site analysis, and high-throughput chromatin immunoprecipitation assays to identify direct and biologically relevant targets of Hoxc8 in vivo. Here we show that 19 of the 34 responsive genes contain Hoxc8 consensus DNA-binding sequence(s) in their regulatory regions. Chromatin immunoprecipitation analysis indicated that Hoxc8-DNA interaction was detected in five of the 19 candidate genes. All of these five target genes have been implicated in oncogenesis, cell adhesion, proliferation, and apoptosis. Overall, the genes described here should aid in the understanding of global regulatory networks of Hox genes and to provide valuable insight into the molecular basis of Hoxc8 in development and carcinogenesis.cancer ͉ chromatin immunoprecipitation ͉ Hox protein binding sites ͉ microarray

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Section: Ncammentioning

confidence: 99%

“…Specific roles for Hoxb3 and Hoxd3 in cell proliferation have been reported during hematopoesis and angiogenesis (8,9). Additionally, Hoxa5 and Hoxa10 regulate P53 expression in human breast cancer cells (10,11).…”

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confidence: 99%

Identification of aHoxc8-regulated transcriptional network in mouse embryo fibroblast cells

Lei

Juan

Kim

et al. 2006

Proc. Natl. Acad. Sci. U.S.A.

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“…HOXA10 has a role in invasion of breast cancer cells 27 and HOXB13 has been shown to be associated with differentiation of epidermal tissues. 28 These data show that the HOX genes have multiple roles in human developmental processes and the association of HOX genes with several types of cancers may be one of the functions of HOX genes.…”

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confidence: 99%

Expression pattern of the class I homeobox genes in ovarian carcinoma

et al. 2010

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Objective: Although some sporadic reports reveal the link between the homeobox (HOX) genes and ovarian carcinoma, there is no comprehensive analysis of the expression pattern of the class I homeobox genes in ovarian carcinoma that determines the candidate genes involved in ovarian carcinogenesis. Methods: The different patterns of expression of 36 HOX genes were analyzed, including 4 ovarian cancer cell lines and 4 normal ovarian tissues. Using a reverse transcription-polymerase chain reaction (RT-PCR) and quantification analysis, the specific gene that showed a significantly higher expression in ovarian cancer cell lines than in normal ovaries was selected, and western blot analysis was performed adding 7 ovarian cancer tissue specimens. Finally, immunohistochemical and immunocytochemical analyses were performed to compare the pattern of expression of the specific HOX gene between ovarian cancer tissue and normal ovaries. Results: Among 36 genes, 11 genes had a different level of mRNA expression between the cancer cell lines and the normal ovarian tissues. Of the 11 genes, only HOXB4 had a significantly higher level of expression in ovarian cancer cell lines than in normal ovaries (p=0.029). Based on western blot, immunohistochemical, and immunocytochemical analyses, HOXB4 was expressed exclusively in the ovarian cancer cell lines or cancer tissue specimens, but not in the normal ovaries. Conclusion:We suggest HOXB4 may be a novel candidate gene involved in ovarian carcinogenesis.

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“…Moreover, there is evidence that HOX, MEIS and PBX genes are involved in oncogenic processes, such as chromatin binding, cell cycle control, proliferation, apoptosis, angiogenesis and cell-cell communications. 3,7,25,28,[32][33][34][35][36][37][38][39] It has been shown that in the normal endometrium MEIS1 protein was expressed in early proliferative glandular epithelium and was absent throughout the rest of the cycle, suggestive of a function in proliferation for MEIS1. 25 Furthermore, after exposure of the ovarian surface epithelium cell line MCV152 to follicle-stimulating hormone, cell proliferation was increased and MEIS1 expression was up-regulated.…”

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confidence: 99%