HPLC Analysis and Molecular Docking Study of Myoporum serratum Seeds Extract with Its Bioactivity against Pathogenic Microorganisms and Cancer Cell Lines

Abstract

Natural constituents have been utilized to avoid humanity from various diseases, such as microbial infection and cancer, over several decades due to bioactive compounds. Myoporum serratum seeds extract (MSSE) was formulated via HPLC for flavonoid and phenolic analysis. Moreover, antimicrobial via well diffusion method, antioxidant via 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging method, anticancer activities against HepG-2 cells (human hepatocellular cancer cell line), and MCF-7 cells (human breast cancer cell line), and molecular docking of the main detected flavonoid and phenolic compounds with the cancer cells were performed. The phenolic acids, including cinnamic acid (12.75 µg/mL), salicylic acid (7.14 µg/mL), and ferulic (0.97 µg/mL), while luteolin represents the main detected flavonoid with a concentration of 10.74 µg/mL, followed by apegenin 8.87 µg/mL were identified in MSSE. Staphylococcus aureus, Bacillus subtilis, Proteus vulgaris, and Candida albicans were inhibited by MSSE with 24.33, 26.33, 20.67, and 18.33 mm of inhibition zone, respectively. MSSE exhibited a low inhibition zone of 12.67 mm against Escherichia coli while showing no inhibitory activity against Aspergillus fumigatus. The values of MIC ranged from 26.58 to 136.33 µg/mL for all tested microorganisms. MBC/MIC index and cidal properties were attributed to MSSE for all tested microorganisms except E. coli. MSSE demonstrated anti-biofilm 81.25 and 50.45% of S. aureus and E. coli, respectively. IC50 of the antioxidant activity of MSSE was 120.11 µg/mL. HepG-2 and MCF-7 cell proliferation were inhibited with IC50 140.77 ± 3.86 µg/mL and 184.04 µg/mL, respectively. Via Molecular docking study, luteolin and cinnamic acid have inhibitory action against HepG-2 and MCF-7 cells, supporting the tremendous anticancer of MSSE.

Anti-Helicobacter pylori, Antioxidant, Antidiabetic, and Anti-Alzheimer’s Activities of Laurel Leaf Extract Treated by Moist Heat and Molecular Docking of Its Flavonoid Constituent, Naringenin, against Acetylcholinesterase and Butyrylcholinesterase

“…As mentioned previously, H. pylori was inhibited by L. nobilis leaf extract with an inhibition zone of 24 mm at 200 mg/mL [16]. Secondary metabolites of the plant have been recommended as possible alternatives for the treatment of H. pylori as well as other microbes [17][18][19][20][21][22]. Alzheimer's disease (AD) is a chronic neurodegenerative disease that is considered one of the most common health issues in the world, and its prevalence is increasing among the elderly population [23].…”

Green Synthesis and Characterization of Titanium Dioxide Nanoparticles by Aspergillus niger DS22 and Its Potential Application in Medical Fields

“…These peaks (which consisted of compounds like 1,2,3,4,6-Pentatrimethylsilyl Glucopyranose, Trimethylsilyl ether-glucitol and Octadecanamide, N-(2-methyl propyl)-N-nitroso) could be attributable to amino acid residues in protein molecules, since these compounds are linear nitrogenous compounds have long carbon chains (C16-C18) which can contribute with better interaction and can be protonated in the presence of other compounds (Rocha et al, 2020), indicating the production of certain proteinic substances into cell-free filtrate of Aspergillus niger DS22 (ON076463.1). On the other side, as a result of its anti-angiogenesis implications and controlling functions of signal pathways such as autophagy, apoptosis, and the arrest of the cell cycle of cancer cells, oridonin is frequently utilized for relieving pain and inflammation associated with cancer such as carcinoma of the liver, cancer of the breast, carcinoma of the gall bladder, cervical carcinoma, Several bioactive Compared with other fungi, Aspergillus species is one of the most prevalent Antiangiogenic, antioxidant, and antitumorigenic effectiveness (Niero et al, 2013;Mashraqi et al, 2023).…”

“…Many biological activities were documented in other investigations utilizing these flavonoid and phenolic compounds. For instance, antiangiogenic, antioxidant, and antitumorigenic effectiveness were attributed to cinnamic acid (Niero et al, 2013;Mashraqi et al, 2023). Gallic acid has been investigated to have anti-HCV activity, and immunomodulatory effects (Bai et al, 2021;Mashraqi et al, 2023).…”

“…For instance, antiangiogenic, antioxidant, and antitumorigenic effectiveness were attributed to cinnamic acid (Niero et al, 2013;Mashraqi et al, 2023). Gallic acid has been investigated to have anti-HCV activity, and immunomodulatory effects (Bai et al, 2021;Mashraqi et al, 2023). Moreover, Ellatif et al (2022) illustrated that 4, 3 indul butyl acetic acid displayed several therapeutic activities such as antidiabetic, anticancer, antibacterial, anti-HIV, antiviral, antiinflammatory, and antioxidant activities.…”