HPLC Analysis of Polyphenolic Compounds and Antioxidant Activity in Nasturtium officinale

Mostafazadeh

et al. 2013

Pharmaceutical Biology

Context: Nasturtium officinale R. Br. (watercress) has long been used in Iranian folk medicine to treat hypertension, hyperglycemia, and renal colic. Moreover, anticancer, antioxidant, and hepatoprotective properties of N. officinale have been reported. Objective: In this study, anti-inflammatory activity of the hydro-alcoholic extract from aerial parts of N. officinale was investigated. Materials and methods: Oral administration of the hydro-alcoholic extract of N. officinale (250, 500 and 750 mg kg À1) was investigated on two well-characterized animal models of inflammation, including carrageenan-or formalin-induced paw edema in rats. Then, the topical anti-inflammatory effect of N. officinale (2 and 5 mg/ear) was studied on 12-O-tetradecanoylphorbol-13-acetate (TPA)-induced mouse ear edema. Finally, biopsy of the paw or ear was performed for pathological evaluation. Results: Acute toxicity tests of N. officinale in rats established an oral LD 50 of 45 g kgThe extract of watercress (250, 500 and 750 mg kg À1) significantly inhibited carrageenaninduced paw edema 1, 2, 3 and 4 h after carrageenan challenge (p50.001). The extract (500 mg kg À1) also showed considerable activity against formalin-evoked paw edema over a period of 24 h (p50.001). Furthermore, topical application of N. officinale (5 mg/ear) reduced TPA-induced ear edema (p50.05). Histopathologically, the extract decreased swelling and the tissue damage induced by carrageenan or TPA. Discussion and conclusion: Our findings indicate potent anti-inflammatory activity of N. officinale in systemic and topical application and propose its potential as an anti-inflammatory agent for treatment of inflammatory conditions.

Section: Discussionmentioning

confidence: 99%

In vivoanti-inflammatory properties of aerial parts ofNasturtium officinale

Mostafazadeh

et al. 2013

Pharmaceutical Biology

Journal of Apicultural Science

“…Several studies describe such biological activities of plants as antibacterial, antifungal, and antiviral. These activities are related to the presence of a diverse group of chemical compounds including phenolics, flavonoids, glycosides, steroids, and alkaloids (Boligon et al, 2012a;2012b;2013b).…”

mentioning

confidence: 99%

Potential Use of Buddleja Thyrsoides for the Control and Prevention of American Foulbrood Disease in Honey Bees

Boligon

Piana

Brum

et al. 2014

a b s t r a c t Paenibacillus larvae is the causative agent of american foulbrood (afb), a severe disease that affects the larvae of the honeybees. the use of plant extracts are considered to be an alternative way of controlling the disease. in this study, the in vitro antimicrobial activity of buddleja thyrsoides lam. against the Paenibacillus species, including P. larvae, was evaluated. in mueller-hinton broth, the minimal inhibitory concentration (miC) was assessed using the microdilution method. all Paenibacillus species were sensitive to the crude extract and the fractions of b. thyrsoides. the ethyl acetate (ea) fraction showed a better result with miC values of 1.68 -3.36 mg/ml, followed by butanolic (bU) (miC = 2.18 -6.54 mg/ml), dichloromethane (dCm) (7.40 -14.80 mg/ml), and crude extract (Ce) (7.51 -16.90 mg/ml). the toxic effect of the Ce and fractions of b. thyrsoides against bees were also evaluated using the spraying application method with the same concentrations of miCs. bee mortality was evident in treatment with dCm fractions only, while Ce, ea, and bU extracts showed no toxic effects after 15 days of observation. furthermore, phenolic acids, tannins, and flavonoids were identified and quantified by highperformance liquid chromatography (hPlC), and may be partially responsible for the antimicrobial properties observed. these results show, for the first time, that b. thyrsoides might be a natural alternative for the prevention/control of afb.

“…-induced lipid peroxidation in rat brain homogenates. HPLC-DAD analysis detected rutin, chlorogenic acid, and caffeic acid in the hydroethanolic extract of N. officinale (Boligon et al 2013).…”

Section: Nasturtium Officinalementioning

confidence: 99%

Edible freshwater macrophytes: a source of anticancer and antioxidative natural products—a mini-review

et al. 2015

Edible freshwater macrophytes (EFM) are edible, macroscopic freshwater plants, many of which are also used as traditional/folk medicine. This minireview highlights phytochemical and pharmacological evidence pertaining to anticancer and antioxidative natural products derived from EFM, with special attention to Centella asiatica (Indian pennywort), Nelumbo nucifera (sacred lotus), Nasturtium officinale (watercress), Ipomoea aquatica (water spinach) and Ludwigia adscendens (water primrose). Current knowledge gaps and further research opportunities are also discussed. EFM is a promising source of anticancer and antioxidative natural products which warrants more extensive exploration. More research is needed before such natural products can be exploited for application in food and medicine.