HPLC‐DAD Analysis, Antioxidant and Protective Effects of Tunisian Rhanterium suaveolens against Acetamiprid Induced Oxidative Stress on Mice Erythrocytes

Abstract

The present study was performed to assess the HPLC-DAD analysis as well as antioxidant and protective effects of Tunisian Rhanterium suaveolens (Rs) against acetamiprid (ACT) induced oxidative stress on mice erythrocytes. The in vitro assays showed that the methanolic extract of Rs has an impressive antioxidant effect proved by testing the total antioxidant and scavenging activities using BCB, DPPH and ABTS assays, respectively. Moreover, qualitative and quantitative analysis using HPLC-DAD revealed the richness of Rs in polyphenols where p-Coumaric, Apigenin-7-glucoside and Ferulic acid were detected as the most abundant polyphenols. In the in vivo experiment, ACT, used as a toxicity model, was given to mice at a dose of 20 mg/kg. The latter was the origin of hemolytic anemia characterized by a significant decrease in red blood cells, hemoglobin and hematocrit levels and an increase in bilirubin, LDH, osmotic fragility, reticulocytes and white blood cells number. Characteristic erythrocyte morphological alterations were also determined as spherocytosis, schistocytosis and dacryocystitis. The oxidative status of ACT-treated mice was also altered manifested by a significant increase in MDA and GSH levels and a decrease in SOD, CAT and GPx activities. When receiving the Rs methanolic extract at a dose of 300 mg/kg, all the parameters cited above were restored in mice. These remarkable corrections could only confirm the important antioxidant effect and the noticeable protective properties that possess Rs owing to its broad range of secondary bioactive metabolites.

Electrochemical Detection of Dopamine and Riboflavine on a Screen-Printed Carbon Electrode Modified by AuNPs Derived fromRhanterium suaveolensPlant Extract

“…First, the methanolic extract of R. suaveolens was obtained as described in detail in Section 3 and in a previous paper. 18 Then, it was added, drop by drop and under stirring conditions, to the solution of AuCl 4 − ions. Initially, the HAuCl 4 solution had a pale yellow color and a pH of 4.5.…”

“…The profile of these phenolic compounds has been reported in a previous paper. 18 Some of these, such as pcoumaric acid and ferulic acid, are present in high amounts and can act as reducing and stabilizing agents during the synthesis of AuNPs. 19 AuNPs play a key role in the recent advances in biomedical applications.…”

Chemical Composition, Antioxidant and Enzyme Inhibitory Activities of Onosma bourgaei and Onosma trachytricha and in Silico Molecular Docking Analysis of Dominant Compounds

“…In another study investigating the antioxidant and protective effects of Rhanterium suaveolens on mouse erythrocytes against acetamiprid (ACT)-induced oxidative stress, significant increases in SOD, CAT, and GPx activities were found in mice treated with R. suaveolens at a dose of 300 mg/kg. HPLC-DAD analysis showed that one of the main compounds of the extract was apigenin 7-glucoside and this compound can affected the activity positively [ 32 ].…”

Evaluation of Antioxidant, Anti‐Inflammatory and Antityrosinase Potential of Extracts from Different Aerial Parts ofRhanterium suaveolensfrom Tunisia

“…Herbarium voucher specimen (number: 155/2019) was deposited at the Faculty of Science (Sfax, Tunisia). The methanolic extracts from Rs flowers, stems and leaves were prepared as previously described by Chelly et al [12] …”

“…Although, the extracts from the whole aerial part of Rhanterium suaveolens (Rs) was already studied, [9,12] to the best of our knowledge no study has been carried out on those obtained from different Rs organs (flowers, stems, and leaves).…”