HPLC-fluorimetric method for analysis of amino acids in products of the hive (honey and bee-pollen)

González-Paramás, Ana M.; Bárez, Juan Alfonso Gómez; Marcos, Carlos Cordón; García-Villanova, Rafael J.; Sánchez, José Sánchez

doi:10.1016/j.foodchem.2005.02.008

Cited by 147 publications

(64 citation statements)

References 18 publications

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“…Isoleucine is often deficient in pollen, especially eucalypt pollens (Somerville and Nicol, 2006). Most analyses have been of bound amino acids, and separate analyses of water-soluble and protein-bound amino acids show that the latter are dominant in terms of concentration (González Paramás et al, 2006;Weiner et al, 2010). Analysis of free amino acids in a single sample of hand-collected Monsanto DK4040 pollen (data not shown) gave a total amino acid concentration that was only 2% of the concentration of protein-bound amino acids.…”

Section: Discussionmentioning

confidence: 94%

Chemical composition of the ‘low quality’ pollen of sunflower (Helianthus annuus, Asteraceae)

2012

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-The nutritional needs of bees are receiving renewed attention in the context of declining bee populations and changes in land use that threaten floral resources. We present a comprehensive analysis of the nutritional composition of sunflower (Helianthus annuus) pollen, comparing hand-collected, bee-collected and stored pollen. As found in previous studies, the protein content of sunflower pollen was relatively low compared to other important bee forage plants. In the cultivars tested two essential amino acids, methionine and tryptophan, are likely to be below the minimum requirements for honeybees. Fatty acid composition showed lauric acid to be most abundant, followed by palmitic and α-linolenic acids. While sunflower offers abundant and accessible pollen, its quality may hinder bee development when it is an exclusive pollen source, and the cultivars of such mass-flowering crops may vary in value for pollinators.

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Section: Discussionmentioning

confidence: 94%

Chemical composition of the ‘low quality’ pollen of sunflower (Helianthus annuus, Asteraceae)

2012

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“…Proline, an essential free amino acid used for quality control of honey samples (Paramás, Bárez, Marcos, García‐Villanova, & Sánchez, 2006). Values below 180 mg/100 g may indicate the none ripeness of a honey sample and/or adulteration (Bogdanov et al., 1999).…”

Section: Resultsmentioning

confidence: 99%

Physicochemical characterization and antioxidant activity of Palestinian honey samples

Imtara

Elamine

Lyoussi

2018

Food Science & Nutrition

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Physicochemical characteristics, main minerals, and antioxidant activity were determined for Palestinian honey samples belonging to different floral and geographical origins. The features of the analyzed samples were within the established international standards for honey quality control. One clear exception was the hydroxymethylfurfural (HMF) of the Ziziphus sample purchased from the Jericho region, which is the lowest city in the word characterized by a hot desert climate. The observed HMF value was 81.86 ± 2.64 mg/kg being two folds the maximum allowed in honey samples (40 mg/kg). As a second objective of the present work, the parameters were divided into two groups with different discriminatory power. The assessed physicochemical parameters, and the antioxidant activities, specific to the botanical origin discrimination, were used to run the first PCA. A strong correlation could be seen between the bioactive compounds and the antioxidant activities despite the geographical origin of the samples. Thyme and Ziziphus samples were the best samples, while citrus sample presented the lowest activity. Regarding the geographical discrimination, Ash and mineral contents in addition to the electrical conductivity were used. The output PCA conserved high represent ability of the data in the two‐first components being 82.72% and 9.60%. A little discrimination between the samples produced in the north and those produced in the south of the country, but it was not perfect. The intervention of the botanical variability could be the reason.

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“…Its composition includes proteins, lipids, fibers (MARCHINI et al, 2006), minerals (FUNARI et al, 2003), vitamins C andE andβ-carotene (ALMEIDA-MURADIAN et al, 2005;OLIVEIRA et al, 2009), free amino acids (PARAMAS et al, 2006), sugars (QIAN et al, 2008), unsaturated fatty acids (FEAS et al, 2012), and phenolic compounds (MARGHITAS et al, 2009).…”

Section: Introductionmentioning

confidence: 99%

Physicochemical characteristics of pollen collected by Amazonian stingless bees

2016

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The purpose of this study was to determine the physicochemical characteristics of pollen collected by the Amazonian stingless bees Melipona seminigra and Melipona interrupta, in order to verify whether their characteristics meet the physicochemical requirements established by the Brazilian Technical Regulation for Identity and Quality of Bee Pollen.Physicochemical analyses were performed through official analytical methods. Results of pollen analyses collected by M. seminigra and M. interrupta were respectively as follows: moisture: 53.39 and 37.12%; protein: 37.63 and 24.00%; lipids: 10.81 and 6.47%; ash: 4.03 and 2.74%; crude fiber: 9.30 and 13.65%; carbohydrates: 25.66 and 44.27%; energy: 350.47 and 331.33kcal%; pH: 3.70 and 3.34; total solids: 46.60 and 62.87%, and water activity: 0.91 and 0.85 foram, respectivamente: umidade: 53,39 e 37,12%; proteínas: 37,63 e 24,00%; lipídeos: 10,81 e 6,47%; cinzas: 4,03 e 2,74%; fibra bruta: 9,30 e 13,65%; carboidratos: 25,66 e 44,27%; energia: 350,47 e 331,33kcal%; pH: 3,70 e 3,34; sólidos totais: 46,60

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HPLC-fluorimetric method for analysis of amino acids in products of the hive (honey and bee-pollen)

Cited by 147 publications

References 18 publications

Chemical composition of the ‘low quality’ pollen of sunflower (Helianthus annuus, Asteraceae)

Chemical composition of the ‘low quality’ pollen of sunflower (Helianthus annuus, Asteraceae)

Physicochemical characterization and antioxidant activity of Palestinian honey samples

Physicochemical characteristics of pollen collected by Amazonian stingless bees

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