HPLC Method for Plasma Vitamin K1: Effect of Plasma Triglyceride and Acute-Phase Response on Circulating Concentrations

Azharuddin, Mohammed; O’Reilly, Denis St. J.; Gray, Andrew; Talwar, Dinesh

doi:10.1373/clinchem.2007.086280

Cited by 35 publications

(26 citation statements)

References 38 publications

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“…Intra-and interday repeatability was adequate as well as recovery testing. For the serum, a limit of detection of 0.03 nmol/L (0.015 ng/mL) was found, which indicates about 3-fold higher sensitivity compared to assays, previously reported [20,30,[32][33][34].…”

Section: Bioanalytical Methods Validation Of Vitamin K1 and Mk-4 Serummentioning

confidence: 68%

A Validated HPLC Method for the Determination of Vitamin K in Human Serum – First Application in a Pharmacological Study

Marinova¹,

Lütjohann

Westhofen³

et al. 2011

TOCCHEMJ

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Dietary phylloquinone (vitamin K1) is considered to be a major determinant of human vitamin K status. For this reason, measurements of plasma vitamin K concentrations provide a useful tool of vitamin K status in man. There is a growing interest in the role, biochemical function, and metabolism of vitamin K in vivo.A modified reversed phase-HPLC method with fluorescence detection after post-column zinc reduction in serum samples was validated for vitamin K analysis. Two human volunteers were investigated after p.o. and i.v. administration of 2 mg Konakion ® MM. Blood samples were collected periodically after administration and subjected to pharmacokinetic evaluation.High sensitivity, analytical recoveries, accuracy and calibration curves linearities could be reached. Our results showed an overall coefficient of variation of less than 10% for the intra-and interday reproducibility, while the recovery ranged from 91% to 114 %. The limit of detection and quantification were 0.015 ng mL -1 and 0.15 ng mL -1 , respectively. Long-term stability was verified over a period of six months. The accuracy was proven by good results from external quality assurance.The observed pharmacokinetic differences indicate significant intra-and inter-individual variance of vitamin K fate in the human body.This highly accurate, robust and reliable method is appropriate for the evaluation of vitamin K status in human serum. The ability to determine vitamin K has the potential to improve pharmacokinetic studies.

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Section: Bioanalytical Methods Validation Of Vitamin K1 and Mk-4 Serummentioning

confidence: 68%

A Validated HPLC Method for the Determination of Vitamin K in Human Serum – First Application in a Pharmacological Study

Marinova¹,

Lütjohann

Westhofen³

et al. 2011

TOCCHEMJ

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“…SPE, in addition to its use as a cleanup method, is performed to concentrate the samples. C18 and Oasis HLB cartridges are the most commonly used during sample preparations (Azharuddin et al 2007;Karpinska et al 2006;Vainchtein et al 2008).…”

Section: Analytical Methods For the Detection Of Quinonesmentioning

confidence: 99%

“…Detection methods such as UV (Fahmy et al 2004;Ojha et al 2009;Qian et al 2008;Xue et al 2008), chemiluminescence (CL) (Ahmed et al 2007;Ahmed et al 2009), and fluorescence (Azharuddin et al 2007), have been combined to HPLC methods. Several quinones can be detected by chemiluminescence due to their ability to generate hydrogen peroxide and a fluorophore when subjected to UV irradiation, a property that allows their determination by mixing with aryloxalate through peroxyoxalate chemiluminescence (PO-CL) reaction (Ahmed et al 2007).…”

Section: Analytical Methods For the Detection Of Quinonesmentioning

confidence: 99%

“…In addition Coenzyme Q (7) acts as a powerful antioxidant and membrane stabilizer, prevents cellular damage resulting from normal metabolic processes (Nageswara Rao et al 2008), and protects against several chronic diseases, including Parkinson's and cardiovascular diseases (Cleren et al 2008;Pepe et al 2007). Vitamin K (8a,b) is essential to maintain life by its function in blood coagulation processes (Ahmed et al 2007;Azharuddin et al 2007;Benzakour 2008), in preventing cardiovascular disease (Beulens et al 2008;Wallin et al 2008), as well as in the prevention and treatment of osteoporosis (Bugel 2008;Lanham-New 2008;Weber 2001). Moreover, many quinones have antioxidant [vitamin E (a-tocopherol) (9)], anti-inflammatory [vitamin E (a-tocopherol) (9)], thymoquinone (10), anthraquinone-2,6-disulfonic acid (11)), antibiotic [phaeosphenone (12)], antimicrobial [anthraquinones (4)], and anticancer [thymoquinone (10), Tanshinone IIA (13), emodin (14), doxorubicin (15)] activities (AlvarezCedron et al 1999;Atasayar et al 2008;Clarke et al 2008;Gali-Muhtasib et al 2008a, b;Halamova et al 2010;Koka et al 2010;Lenta et al 2007;Lu et al 2008;Mansour and Tornhamre 2004;Mazuel et al 2003;Rahman et al 2008;Rizzo et al 2008;Savarino et al 2007;Sethi et al 2008;Sottani et al 2008;Su et al 2008;Takahashi et al 2008;Zhang et al 2008;Zhu et al 2007).…”

Section: Introductionmentioning

confidence: 99%

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The chemical and biological activities of quinones: overview and implications in analytical detection

et al. 2011

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Quinones are a class of natural and synthetic compounds that have several beneficial effects. Quinones are electron carriers playing a role in photosynthesis. As vitamins, they represent a class of molecules preventing and treating several illnesses such as osteoporosis and cardiovascular diseases. Quinones, by their antioxidant activity, improve general health conditions. Many of the drugs clinically approved or still in clinical trials against cancer are quinone related compounds. Quinones have also toxicological effects through their presence as photoproducts from air pollutants. Quinones are fast redox cycling molecules and have the potential to bind to thiol, amine and hydroxyl groups. The aforementioned properties make the analytical detection of quinones problematic. However, recent advances of the available analytical techniques along with the possibility of using labeled compound facilitate their detection hence allowing a better understanding of their action. This review summarizes the current knowledge with respect to the oxido-reductive and electrophilic properties of quinones as well as to the analytical tools used for their analysis. It includes a general introduction about the physiological, and therapeutical functions of quinones. A number of studies are reported to cover the chemical reactivity in an attempt to understand quinones as biologically active compounds. Data ranging from normal analytical methods to study quinones derived from plant or biological matrices to the use of labeled compounds are presented. The examples illustrate how chemical, biological and analytical knowledge can be integrated to have a better understanding of the mode of action of the quinones.

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“…Different phylloquinone and menaquinones plasma concentrations have been reported, with a possible influence of dietary intake, and a possible analytic interference from triglycerides [7]. Mean plasma concentrations ranging from 0.22 to 8.88 nmol/L have been reported, although in most studies phylloquinone had a concentration below 2 nmol/L [8].…”

Section: Introductionmentioning

confidence: 99%

Vitamin K plasma levels determination in human health

Fusaro

Gallieni

Rizzo

et al. 2017

Clinical Chemistry and Laboratory Medicine (CCLM)

105

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Vitamin K (phylloquinone or vitamin K 1 and menaquinones or vitamin K 2 ) plays an important role as a cofactor in the synthesis of hepatic blood coagulation proteins, but recently has also aroused an increasing interest for its action in extra-hepatic tissues, in particular in the regulation of bone and vascular metabolism. The accurate measurement of vitamin K status in humans is still a critical issue. Along with indirect assays, such as the undercarboxylated fractions of vitamin K-dependent proteins [prothrombin, osteocalcin (OC), and matrix gla protein], the direct analysis of blood levels of phylloquinone and menaquinones forms might be considered a more informative and direct method for assessing vitamin K status. Different methods for direct quantification of vitamin K serum levels are available. High-performance liquid chromatography (HPLC) methods coupled with post-column reduction procedures and fluorimetric or electrochemical detection are commonly used for food and blood analysis of phylloquinone, but they show some limitations when applied to the analysis of serum menaquinones because of interferences from triglycerides. Recent advancements include liquid chromatography tandem mass spectrometry (LCMS/MS) detection, which assures higher specificity. The optimization and standardization of these methods requires specialized laboratories. The variability of results observed in the available studies suggests the need for further investigations to obtain more accurate analytical results.

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HPLC Method for Plasma Vitamin K1: Effect of Plasma Triglyceride and Acute-Phase Response on Circulating Concentrations

Cited by 35 publications

References 38 publications

A Validated HPLC Method for the Determination of Vitamin K in Human Serum – First Application in a Pharmacological Study

A Validated HPLC Method for the Determination of Vitamin K in Human Serum – First Application in a Pharmacological Study

The chemical and biological activities of quinones: overview and implications in analytical detection

Vitamin K plasma levels determination in human health

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