hprt mutation frequency among workers exposed to 1,3-butadiene in China

Hayes, Richard B.; Xi, Liqiang; Bechtold, William E.; Rothman, Nataniel; Yao, Ming; Henderson, Rogene F.; Zhang, Luoping; Smith, Martyn T.; Zhang, Dongpu; Wiemels, Joseph L.; Dosemeci, Mustafa; Yin, Shu; O’Neill, J. Patrick

doi:10.1016/0300-483x(96)03433-6

Cited by 39 publications

(23 citation statements)

References 8 publications

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“…The thioguanine-selected CE divided by the mean unselected CE yields the M f . The results have been reported in detail previously [7].…”

Section: Hprt Mutation Assaymentioning

confidence: 56%

“…Here, we report in detail on part of that investigation: the analysis of cytogenetic changes in chromosomes 1, 7, 8 and 12 using fluorescence in situ hybridization (FISH) and HPRT (hypoxanthine-guanine phosphoribosyl transferase) mutations and their possible correlation with polymorphisms in genes encoding the glutathione S-transferases (GSTs) and microsomal epoxide hydrolase (EPHX1), which metabolize potentially genotoxic BD epoxides. We have previously reported data on HPRT mutant frequency [7], but not in relation to these genotypes.…”

Section: Introductionmentioning

confidence: 94%

“…Identification of facility and enrollment of study subjects has been previously described in detail [7]. At a polybutadiene rubber production facility in Yanshan, China, the purification of BD from an initial hydrocarbon stream occurred at two sites: the DMF facility, where initial distillation and extraction occurred using a proprietary dimethyl formamide (DMF) process; and the recovery facility, where final distillation occurred.…”

Section: Study Populationmentioning

confidence: 99%

“…The HPRT mutant frequency (M f ) was determined by the T cell cloning assay with cryopreserved cell samples as described previously [7]. Briefly, cryopreserved cells were thawed and incubated in medium containing 1 μg/ml PHA (HA17, Wellcome Diagnostics) for 36-40 h to achieve mitogen stimulation.…”

Section: Hprt Mutation Assaymentioning

confidence: 99%

“…To provide mechanistic information on the potential carcinogenicity of BD in humans, we [7][8][9] and others [10][11][12][13][14][15] have investigated genotoxic effects in BD-exposed workers. In a study at a polybutadiene production facility in China, we examined a broad spectrum of genotoxic and other potential effects of BD.…”

Section: Introductionmentioning

confidence: 99%

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Lack of increased genetic damage in 1,3-butadiene-exposed Chinese workers studied in relation to EPHX1 and GST genotypes

Zhang

Hayes

Guo

et al. 2004

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Abstract1,3-Butadiene (BD) is an important industrial chemical and pollutant. Its ability to induce genetic damage and cause hematological malignancies in humans is controversial. We have examined chromosome damage by fluorescence in situ hybridization (FISH) and mutations in the HPRT gene in the blood of Chinese workers exposed to BD. Peripheral blood samples were collected and cultured from 39 workers exposed to BD (median level 2 ppm, 6 h time-weighted average) and 38 matched controls in Yanshan, China. No difference in the level of aneuploidy or structural changes in chromosomes 1, 7, 8, and 12 was detected in metaphase cells from exposed subjects in comparison with matched controls, nor was there an increase in the frequency of HPRT mutations in the BDexposed workers. Because genetic polymorphisms in glutathione S-transferase (GST) enzymes and microsomal epoxide hydrolase (EPHX1) may affect the genotoxic effects of BD and its metabolites, we also related chromosome alterations and gene mutations to GSTT1, GSTM1 and EPHX1 genotypes. Overall, there was no effect of variants in these genotypes on numerical or structural changes in chromosomes 1, 7, 8 and 12 or on HPRT mutant frequency in relation to BD exposure, but the GST genotypes did influence background levels of both hyperdiploidy and HPRT mutant frequency. In conclusion, our data show no increase in chromosomal aberrations or HPRT mutations among workers exposed to BD, even in potentially susceptible genetic subgroups. The study is, however, quite small and the levels of BD exposure are not extremely high, but our findings in China do support those from a similar study conducted in the Czech Republic. Together, these studies suggest that low levels of occupational BD exposure do not pose a significant risk of genetic damage.

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“…The thioguanine-selected CE divided by the mean unselected CE yields the M f . The results have been reported in detail previously [7].…”

Section: Hprt Mutation Assaymentioning

confidence: 56%

Section: Introductionmentioning

confidence: 94%

Section: Study Populationmentioning

confidence: 99%

Section: Hprt Mutation Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

Lack of increased genetic damage in 1,3-butadiene-exposed Chinese workers studied in relation to EPHX1 and GST genotypes

Zhang

Hayes

Guo

et al. 2004

Mutation Research/Genetic Toxicology and Environmental Mutagene

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Aliphatic Hydrocarbons

Carreón¹

2001

Patty's Toxicology

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Aliphatic hydrocarbons are open‐chain compounds that may be saturated or unsaturated. The saturated compounds, known as paraffin hydrocarbons or alkanes , include methane and its homologs having the empirical formula C n H 2 n +2 . The unsaturated compounds fall into a number of homologous series: ( 1 ) those containing one double bond (ethylene and its homologs) and having the formula C n H 2 n are known as olefins or alkenes ; ( 2 ) those containing one triple bond (acetylene and its homologs) are called acetylenes or alkynes and have the formula C n H 2 n −2 ; ( 3 ) those having two double bonds (allene, 1,3‐butadiene, and 1,4‐pentadiene represent three types) are diolefins or alkadienes and also have the formula C n H 2 n −2 ; ( 4 ) those having a large number of double or triple bonds or both double and triple bonds are named in analogous fashion as alkatrienes , alkatetraenes , alkadiynes , alkenynes , and alkadienynes . Aliphatic hydrocarbons are asphyxiants and central nervous system (CNS) depressants. Serious toxic effects of aliphatic hydrocarbons include asphyxia and chemical pneumonitis for many paraffins, axonal neuropathy for n ‐hexane, and cancer for 1,3‐butadiene.

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Aliphatic Hydrocarbons

Carreón¹,

Herrick²

2012

Patty's Toxicology

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Aliphatic hydrocarbons are open‐chain compounds that may be saturated or unsaturated. The saturated compounds, known as paraffin hydrocarbons or alkanes , include methane and its homologs having the empirical formula C n H 2 n +2 . The unsaturated compounds fall into a number of homologous series: ( 1 ) those containing one double bond (ethylene and its homologs) and having the formula C n H 2 n are known as olefins or alkenes ; ( 2 ) those containing one triple bond (acetylene and its homologs) are called acetylenes or alkynes and have the formula C n H 2 n −2 ; ( 3 ) those having two double bonds (allene, 1,3‐butadiene and 1,4‐pentadiene represent three types) are diolefins or alkadienes and also have the formula C n H 2 n −2 ; ( 4 ) those having a large number of double or triple bonds or both double and triple bonds are named in analogous fashion as alkatrienes , alkatetraenes , alkadiynes , alkenynes , and alkadienynes . Aliphatic hydrocarbons are asphyxiants and central nervous system ( CNS ) depressants. Serious toxic effects of aliphatic hydrocarbons include asphyxia and chemical pneumonitis for many paraffins, axonal neuropathy for n ‐hexane, and cancer for 1,3‐butadiene.

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hprt mutation frequency among workers exposed to 1,3-butadiene in China

Cited by 39 publications

References 8 publications

Lack of increased genetic damage in 1,3-butadiene-exposed Chinese workers studied in relation to EPHX1 and GST genotypes

Lack of increased genetic damage in 1,3-butadiene-exposed Chinese workers studied in relation to EPHX1 and GST genotypes

Aliphatic Hydrocarbons

Aliphatic Hydrocarbons

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