HPTLC coupled with bioluminescence and mass spectrometry for bioactivity-based analysis of secondary metabolites in marine sponges

Klöppel, Anne; Grasse, Wolfgang; Brümmer, Franz; Morlock, Gertrud E.

doi:10.1556/jpc.21.2008.6.7

Cited by 41 publications

(19 citation statements)

References 21 publications

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“…In our preliminary attempts for identifying the bioactive components of Crambe crambe , we established HPTLC/bioassay/HRMS and optimized this method for bioactivity‐based analysis of unknown natural products. By this way we were able to demonstrate that methanolic extract of Crambe crambe contains three highly bioactive substances at least 18. Further investigation is needed for identification of the individual Crambe crambe metabolites with anti‐CSC activity.…”

Section: Discussionmentioning

confidence: 80%

“…For species verification, spicula preparations were performed according to Brümmer et al 17. Sponge extract was prepared by methanolic extraction as described 18. In brief, 5 cm 3 of sponge tissue was cut off and frozen immediately in liquid nitrogen (avoidance of biotransformation processes), lyophilized, ground with a mortar and extracted exhaustively overnight in high‐purity MeOH (50 ml/100 mg sponge tissue).…”

Section: Methodsmentioning

confidence: 99%

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Targeting of pancreatic and prostate cancer stem cell characteristics by Crambe crambe marine sponge extract

Ottinger

Klöppel

Rausch

et al. 2011

Intl Journal of Cancer

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Cancer stem cells (CSCs) are suggested as reason for resistance of tumors toward conventional tumor therapy including pancreatic and advanced prostate cancer. New therapeutic agents are urgently needed for targeting of CSCs. Marine sponges harbor novel and undefined compounds with antineoplastic activity but their potential to eliminate CSC characteristics is not examined so far. We collected 10 marine sponges and one freshwater sponge by diving at the seaside and prepared crude methanolic extracts. The effect to established pancreatic and prostate CSC lines was evaluated by analysis of apoptosis, cell cycle, side population, colony and spheroid formation, migratory potential in vitro and tumorigenicity in vivo. While each sponge extract at a 1:10 dilution efficiently diminished viability, Crambe crambe marine sponge extract (CR) still strongly reduced viability of tumor cells at a dilution of 1:1,000 but was less toxic to normal fibroblasts and endothelial cells. CR inhibited self-renewal capacity, apoptosis resistance, and proliferation even in gemcitabine-selected pancreatic cancer cells with acquired therapy resistance and enhanced CSC characteristics. CR pretreatment of tumor cells diminished tumorigenicity of gemcitabine-resistant tumor cells in mice and totally abolished tumor take upon combination with gemcitabine. Our data suggest that CR contains substances, which render standard cancer therapy more effective by targeting of CSC characteristics. Isolation of bioactive metabolites from CR and evaluation in mice are required for development of new CSC-specific chemotherapeutic drugs from a marine sponge.Pancreatic adenocarcinoma and advanced prostate cancer are aggressive malignancies with poor therapeutic options.

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Section: Discussionmentioning

confidence: 80%

Section: Methodsmentioning

confidence: 99%

Targeting of pancreatic and prostate cancer stem cell characteristics by Crambe crambe marine sponge extract

Ottinger

Klöppel

Rausch

et al. 2011

Intl Journal of Cancer

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“…14 The detection of bioactive compounds in complex samples by bacterial assays using the directly bioluminescent marine bacteria Vibrio fischeri has been reported. [15][16][17] Many enzyme substrate reactions that show the viability of microorganism are suited for use in HPTLC bioassay. For instance, in the detection of algicides 18 and fungicides.…”

Section: Microbial Assaymentioning

confidence: 99%

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

Elufioye¹

2017

TJNPR

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“…Currently, there are two bioluminizer systems available for the measurement of luminescence in TLC plates: Bioluminex™ (ChromaDex) and BioLuminizer ® (Camag). These have been applied to detect natural toxins such as mycotoxins and heavy metals and also to evaluate the toxicity of natural secondary metabolites (Klöppel et al, 2008(Klöppel et al, , 2013 or to detect adulterants for the quality control of phytomedicines (Verbitski et al, 2008).…”

Section: Toxicity Bioautographic Assaysmentioning

confidence: 99%

Thin‐layer Chromatography of Plants, with Chemical and Biological Detection Methods

Urbain

Simões-Pires

2014

Encyclopedia of Analytical Chemistry

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Thin‐layer chromatography ( TLC ) is a widespread separation technique in the field of plant analysis and offers multiple advantages. This versatile technique can provide useful information about the qualitative or semiquantitative chemical composition of a mixture, thanks to coupling, with the detection and spectroscopic techniques and devices for image treatment. Moreover, it can contribute to the detection of biological activity by combining chromatography with bioassays, such as antimicrobial or enzyme inhibition tests. This procedure, providing simultaneous chromatographic separation and in situ assessment of a given biological activity, is named TLC bioautography. The major advantage of this technique is the ability to locate active substances from a complex mixture without prior sample purification. This avoids time‐consuming and tedious isolation of already‐known or inactive compounds. As this method is simple and cost effective, it is suitable for preliminary rapid screening to detect potential activity, as well as being a useful monitoring tool to track the bioguided isolation of active components. In this chapter, we focus on up‐to‐date TLC bioautographic assays, detail their mechanisms and applications in the field of natural drug discovery, and discuss the recent advances of this method that might appear at first glance to be a trivial approach.

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HPTLC coupled with bioluminescence and mass spectrometry for bioactivity-based analysis of secondary metabolites in marine sponges

Cited by 41 publications

References 21 publications

Targeting of pancreatic and prostate cancer stem cell characteristics by Crambe crambe marine sponge extract

Targeting of pancreatic and prostate cancer stem cell characteristics by Crambe crambe marine sponge extract

Bioassay-coupled Chromatographic Analysis of Medicinal Natural Products: A Review

Thin‐layer Chromatography of Plants, with Chemical and Biological Detection Methods

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