HPV positivity varies with technique and primer set, in formalin-fixed paraffin-embedded benign and malignant breast tissue from Malta

Abstract

The role of human papillomavirus in breast cancer pathogenesis is an area of considerable debate following differing results from all over the world. This study analyzed paraffin embedded tissue from 19 patients with benign or malignant breast disease. Standard PCR with GP5+/6+ and FAP primers, proved negative in all cases. However, qRT-PCR using the SF10 primer set detected HPV DNA in 2 out of these samples. Whilst our data does not support a major role for HPV in the pathogenesis of breast disease in Malta, it definitely does not exclude it totally. This study also clearly shows that the method of analysis is one of the variables resulting in the range of differing results witnessed worldwide in this area of study

The Prevalence of Human Papilloma Virus among Breast Cancer Women in Relation with IL-8 Level in Kirkuk Province

“…and may be influenced by the technical limits, the epidemiology of HPV in different geographical area, different sexual behavior patterns, differing incidences of ano-genital HPV infection and perhaps different genetics in people could play variety roles in these differing outcomes[29].Fenwick Sh[30] showed in his thesis that the age of patients with HPV+ve results ranged from 29-69 years with the mean age of 49 years. Richardson AK, et al[31], presented in their study that the mean age of breast cancer women was 48 years with greatest in the 40-54 year age-group.…”