HSF1-mediated oxidative stress response to menadione in <i>Saccharomyces cerevisiae</i> KNU5377Y3 by using proteomic approach

Abstract

The hat shock transcription factor HSF1 in the yeast Saccharomyces cerevisiae regulates a wide range of genes and functions in diverse cellular reactions. To investigate the physiological response of HSF1 in the presence of menadione (MD) in S. cerevisiae KNU 5377Y3, wild-type (k3wt) and isogenic hsf1 mutant (k3h1) cells were introduced. HSF1 was induced when k3wt cells were exposed to the superoxide-generating agent MD and k3h1 cells were hypersensitive to MD. Under MD stress, k3h1 cells down-regulated the expression of metabolic enzymes (Hxk, Fba1, Pgk1, Eno2, and Adh1), antioxidant enzymes (Trx2 and porin), and molecular chaperones and their cofactors (Hsp104,

Genetic inactivation of essential HSF1 reveals an isolated transcriptional stress response selectively induced by protein misfolding

“…Heat Shock Factor 1 (Hsf1) is a highly conserved transcription factor in eukaryotes that in response to stress induces a protective transcriptional program called the heat-shock response (HSR) ( Ritossa, 1996 ; Kim et al. , 2013 ; Wang et al.…”

Genetic inactivation of essentialHSF1reveals an isolated transcriptional stress response selectively induced by protein misfolding

“…Heat Shock Factor 1 (Hsf1) is a highly conserved transcription factor in eukaryotes that in response to stress induces a protective transcriptional program called the heat-shock response (HSR) (Ritossa, 1996;Kim et al, 2013;Wang et al, 2013;Steurer et al, 2018). In Saccharomyces cerevisiae, a single and essential gene, HSF1, encodes Hsf1 which is a nuclear protein that functions as a trimeric transcription factor under both basal and stress-inducing conditions (Morano et al, 2012;Verghese et al, 2012).…”