HSF2 Binds to the Hsp90, Hsp27, and c-Fos Promoters Constitutively and Modulates their Expression

Wilkerson, Donald K.; Skaggs, Hollie S.; Sarge, Kevin D.

doi:10.1379/csc-250

Cited by 12 publications

(15 citation statements)

References 30 publications

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“…The results are representative of the banding pattern observed from western blot analysis of two independent mouse sperm protein isolations, and indicate that RNA Polymerase II is present in mature spermatozoa. In both the sperm lane (left image) and C2C12 lane (right image) we observed one predominant band migrating at approximately 220kDa that is consistent with the size of RNA Polymerase II detected in previous studies (Luthi-Carter et al 2002; Wilkerson et al 2007). …”

Section: Resultssupporting

confidence: 90%

“…Previously, we observed in Jurkat cells blocked in mitosis that the binding of RNA Polymerase II to a number of HSE containing promoters was reduced, but not completely eliminated (Wilkerson et al 2007). There was clear and reproducible binding of RNA Polymerase II to the promoters of Hsp90aa1 (Hsp90), fos, and Hspb1 (Hsp27) in mitotic cells.…”

Section: Discussionmentioning

confidence: 98%

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RNA polymerase II interacts with the Hspa1b promoter in mouse epididymal spermatozoa

Wilkerson

Sarge²

2009

Reproduction

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The Hspa1b (Hsp70.1) gene is one of the first genes expressed after fertilization, with expression occurring during the minor zygotic genome activation (ZGA) in the absence of stress. This expression can take place in the male pronucleus as early as the one-cell stage of embryogenesis. The importance of HSPA1B for embryonic viability during times of stress is supported by studies showing that depletion of this protein results in a significant reduction in embryos developing to the blastocyte stage. Recently, we have begun addressing the mechanism responsible for allowing expression of Hspa1b during the minor ZGA and found that heat shock transcription factor (HSF) 1 and 2 bind the Hspa1b promoter during late spermatogenesis. In this report, we have extended those studies using western blots and chromatin immunoprecipitation assays and found that RNA polymerase II (Pol II) is present in epididymal spermatozoa and bound to the Hspa1b promoter. These present results, in addition to our previous results, support a model in which the binding of HSF1, HSF2, SP1, and Pol II to the promoter of Hspa1b would allow the rapid formation of a transcription-competent state during the minor ZGA, thereby allowing Hspa1b expression.

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Section: Resultssupporting

confidence: 90%

Section: Discussionmentioning

confidence: 98%

RNA polymerase II interacts with the Hspa1b promoter in mouse epididymal spermatozoa

Wilkerson

Sarge²

2009

Reproduction

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“…It has been shown that the putative HSF binding site may indirectly modulate the CCR5 expression and thus HIV‐1 replication. Activated HSF2 induces the expression of heat shock protein 70 (HSP70; Wilkerson et al ., ), which in turn stimulates the expression of CCR5 in CD4 T cells and dendritic cells. This leads to increased expression of APOBEC3G enzyme that is known to have antiviral activity via deaminating viral cytidine to uridine and thus making the HIV‐1 virion nonfunctional (Whittall et al ., ; Pido‐Lopez et al ., ).…”

Section: Discussionmentioning

confidence: 99%

New genetic variants in the CCR5 gene and the distribution of known polymorphisms in Omani population

Al-Mahruqi

Zadjali

Koh

et al. 2013

Int J Immunogenetics

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C-C motif chemokine receptor-5 (CCR5) is a pro-inflammatory receptor that binds to chemokines and facilitates the entry of the R5 strain of HIV-1. A number of polymorphisms were identified within the promoter and coding regions of the CCR5 gene, some of which have been found to affect the protein expression and thus receptor function. Although several CCR5 polymorphisms were shown to vary widely in their distribution among different ethnic populations, there has been no study addressing the potential variants of the CCR5 gene in the Omani population. The aim of this study was to identify the polymorphic sites that exist within the CCR5 gene in Omanis. Blood samples were collected from 89 Omani adult individuals, and genomic DNA was amplified by polymerase chain reaction and sequenced to identify the polymorphic sites. The distribution of the detected variants was examined and compared with the previously published data. Four new indels were detected of 32 variable positions, -2973A/-, -2894A/-, -2827TA/- and -2769T/-, and all were located in the 5'UTR. Furthermore, two new mutations, -2248G/A and +658A/G, were observed for the first time; the -2248G/A was detected in the intron 1 region in one subject and +658A/G in the coding region of the CCR5 in another subject. In silico analysis showed that the novel variations in the 5'UTR may have effects on the transcription factor binding sites. Therefore, this study demonstrates the presence of two new SNPs and four novel indels in the CCR5 gene in the Omani population. Our findings support the wide spectrum of genetic diversity reported within the CCR5 gene region among different ethnic groups.

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“…Recently our laboratory has shown that HSF2 can bind to the promoters of Hsp27 , Hsp90 , and C-fos in mitotic cells [57]. Other genes, many of which are members of the heat shock superfamily, are also bound by HSF1 and HSF2 [58].…”

Section: Discussionmentioning

confidence: 99%

Interaction of HSF1 and HSF2 with the Hspa1b Promoter in Mouse Epididymal Spermatozoa1

Wilkerson

Murphy

Sarge

2008

Biology of Reproduction

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The Hspa1b gene is one of the first genes expressed after fertilization, with expression observed in the male pronucleus as early as the one-cell stage of embryogenesis. This expression can occur in the absence of stress and is initiated during the minor zygotic genome activation. There is a significant reduction in embryos developing to the blastocyte stage when HSPA1B levels are depleted, supporting the importance of this protein for embryonic viability. However, the mechanism responsible for allowing expression of Hspa1b during the minor ZGA is unknown. In this report, we investigated the role of HSF1 and HSF2 in bookmarking Hspa1b during late spermatogenesis. Western blots show that both HSF1 and HSF2 are present in epididymal spermatozoa and immunofluorescence analysis revealed that some of the HSF1 and HSF2 proteins in these cells overlap the DAPI-stained DNA region. Results from chromatin immunoprecipitation assays showed that HSF1, HSF2, and Sp1 are bound to the Hspa1b promoter in epididymal spermatozoa. Furthermore, we observed an increase in HSF2 binding to the Hspa1b promoter in late spermatids versus early spermatids suggesting a likely period during spermatogenesis when transcription factor binding could occur. These results support a model in which the binding of HSF1, HSF2, and Sp1 to the promoter of Hspa1b would allow the rapid formation of a transcription-competent state during the minor ZGA, thereby allowing Hspa1b expression.

show abstract

HSF2 Binds to the Hsp90, Hsp27, and c-Fos Promoters Constitutively and Modulates their Expression

Cited by 12 publications

References 30 publications

RNA polymerase II interacts with the Hspa1b promoter in mouse epididymal spermatozoa

RNA polymerase II interacts with the Hspa1b promoter in mouse epididymal spermatozoa

New genetic variants in the CCR5 gene and the distribution of known polymorphisms in Omani population

Interaction of HSF1 and HSF2 with the Hspa1b Promoter in Mouse Epididymal Spermatozoa1

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