Hsp-27 Expression in Invasive Ductal Breast Carcinoma

Grzegrzółka, Jędrzej; Kurnol, Krzysztof; Piotrów, Paweł; Puła, Bartosz; Kobierzycki, Christopher; Piotrowska, Aleksandra; Jabłońska, Karolina; Wojnar, Andrzej; Ryś, Janusz; Dzięgiel, Piotr; Podhorska-Okołów, Marzena

doi:10.5603/fhc.2012.0074

Cited by 11 publications

(3 citation statements)

References 33 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This correlation was further validated by direct in vitro overexpression of HSP27, which indicated that enforced HSP27 promoted the function and the self‐renewal markers of CSC in SACC. This finding is in agreement with several reports, which stated that in other cancers (breast, prostate and ovarian cancer), HSP27 was a marker that was applied to determine negative prognosis .…”

Section: Discussionsupporting

confidence: 93%

HSP27 associates with epithelial–mesenchymal transition, stemness and radioresistance of salivary adenoid cystic carcinoma

Chen

Ren

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

Epithelial–mesenchymal transition (EMT) has been shown to associate with cancer stem cells and radioresistance. However, it is obscure whether EMT itself or specific EMT regulators play causal roles in these properties of salivary adenoid cystic carcinoma (SACC). Here, we exhibited that overexpression of HSP27 drove the migration and invasion, induced EMT, as well as mediated TGF‐β1‐induced EMT in SACC cells, accompanying the up‐regulation of Snail1 and Prrx1. Conversely, HSP27 silencing reduced the migration and invasion and contributed to MET of SACC cells. HSP27 indirectly down‐regulates the expression of E‐cadherin through activating Snail1 and Prrx1 expressions. Overexpression of Snail1 or Prrx1 restored the migration and invasion in HSP27 knockdown cells. Enforced expression of HSP27 enhanced colony formation, CD133+/CD44+ population and radioresistance of SACC cell lines. In addition, HSP27 expression was positively associated with radioresistance and poor prognosis of SACC patients as well as with the expression of Prrx1 or Snail1 in SACC tissues. The data confirm an important function for HSP27 in SACC progression through regulating EMT and stemness, and they imply the possible association between EMT and radioresistance of SACC.

show abstract

Section: Discussionsupporting

confidence: 93%

HSP27 associates with epithelial–mesenchymal transition, stemness and radioresistance of salivary adenoid cystic carcinoma

Chen

Ren

et al. 2018

J Cellular Molecular Medi

View full text Add to dashboard Cite

show abstract

“…Hsps can be classified according to their molecular weight, for example, hsp27, hsp40, hsp60, hsp70, and hsp90. Altered hsp expression has been observed in breast, gastric, and colorectal cancers [Kanazawa et al, ; Partida‐Rodriguez et al, ; Grzegrzolka et al, ], and the knockdown of hsp has been shown to stop tumor migration [Bausero et al, ]. Hsp has also been reported to be a prognostic factor in patients with some tumors such as breast cancer [Thanner et al, ; Lee et al, ].…”

mentioning

confidence: 99%

Expression of Antioxidant Molecules and Heat Shock Protein 27 in Thyroid Tumors

Wang

Yang

Vlantis

et al. 2016

J of Cellular Biochemistry

View full text Add to dashboard Cite

Oxidative stress-induced DNA damage is a known causing factor for many types of tumors, but information on the role of oxidants and antioxidants in thyroid tumors is limited. The aim of this study was to determine antioxidant levels in thyroid tumors. In this study, tumor and its matched non-tumor thyroid tissue samples were obtained from 53 patients with thyroid tumors. The levels of manganese superoxide dismutase (MnSOD), thioredoxin reductase 2 (TXNRD2), glutathione (GSH), glutathione peroxidase (Gpx), catalase (CAT), and 27 kd heat-shock protein (hsp27) were determined in both thyroid tissue samples and cultured thyroid cells by immunohistochemical staining and western blot. Hydrogen peroxide (H2 O2 ) was used to generate oxidant stress in the cell culture experiments. We found that the levels of MnSOD, TXNRD2, GSH, Gpx, and Hsp27 were increased in both malignant and benign tumors, while the level of CAT was decreased. To verify the results of the tissue study, we treated cultured thyroid cells with H2 O2 and found the same pattern of antioxidant changes. Hsp27 was also increased after H2 O2 treatment. The expression of hsp27 was upregulated by 8.24-, 6.96-, and 3.09-fold in thyroid cancer, follicular adenoma, multinodular goiter, respectively. Collectively, our study demonstrated that the levels of hsp27 together with MnSOD, TXNRD2, GSH, and Gpx were significantly upregulated by H2 O2 in thyroid tumors. The increase of these antioxidants is observed in both malignant and benign tumors, particularly in the former. The upregulation of antioxidants is likely a protective mechanism of tumor cells to maintain their survival and growth. J. Cell. Biochem. 117: 2473-2481, 2016. © 2016 Wiley Periodicals, Inc.

show abstract

“…Hsp60 and Hsp90 are present on the surface of tumor cells and are secreted by these cells via exosomes, which makes these two chaperones promising, easily accessible biomarkers [ 114 , 115 ]. Likewise, the chaperones Hsp10 and Hsp27 have also been implicated in multifunctional chaperone networks in invasive BC [ 116 ].…”

Section: Hsp27 Hsp60 Hsp70 and Hsp90 In Bc Therapeutics: Negative Cha...mentioning

confidence: 99%

The Chaperone System in Breast Cancer: Roles and Therapeutic Prospects of the Molecular Chaperones Hsp27, Hsp60, Hsp70, and Hsp90

Alberti

Vergilio

Paladino

et al. 2022

IJMS

View full text Add to dashboard Cite

Breast cancer (BC) is a major public health problem, with key pieces of information needed for developing preventive and curative measures still missing. For example, the participation of the chaperone system (CS) in carcinogenesis and anti-cancer responses is poorly understood, although it can be predicted to be a crucial factor in these mechanisms. The chief components of the CS are the molecular chaperones, and here we discuss four of them, Hsp27, Hsp60, Hsp70, and Hsp90, focusing on their pro-carcinogenic roles in BC and potential for developing anti-BC therapies. These chaperones can be targets of negative chaperonotherapy, namely the elimination/blocking/inhibition of the chaperone(s) functioning in favor of BC, using, for instance, Hsp inhibitors. The chaperones can also be employed in immunotherapy against BC as adjuvants, together with BC antigens. Extracellular vesicles (EVs) in BC diagnosis and management are also briefly discussed, considering their potential as easily accessible carriers of biomarkers and as shippers of anti-cancer agents amenable to manipulation and controlled delivery. The data surveyed from many laboratories reveal that, to enhance the understanding of the role of the CS in BS pathogenesis, one must consider the CS as a physiological system, encompassing diverse members throughout the body and interacting with the ubiquitin–proteasome system, the chaperone-mediated autophagy machinery, and the immune system (IS). An integrated view of the CS, including its functional partners and considering its highly dynamic nature with EVs transporting CS components to reach all the cell compartments in which they are needed, opens as yet unexplored pathways leading to carcinogenesis that are amenable to interference by anti-cancer treatments centered on CS components, such as the molecular chaperones.

show abstract

Hsp-27 Expression in Invasive Ductal Breast Carcinoma

Cited by 11 publications

References 33 publications

HSP27 associates with epithelial–mesenchymal transition, stemness and radioresistance of salivary adenoid cystic carcinoma

HSP27 associates with epithelial–mesenchymal transition, stemness and radioresistance of salivary adenoid cystic carcinoma

Expression of Antioxidant Molecules and Heat Shock Protein 27 in Thyroid Tumors

The Chaperone System in Breast Cancer: Roles and Therapeutic Prospects of the Molecular Chaperones Hsp27, Hsp60, Hsp70, and Hsp90

Contact Info

Product

Resources

About