Hsp genes are differentially expressed during Trichoderma asperellum self-recognition, mycoparasitism and thermal stress

Mota, Thuana Marcolino; Oshiquiri, Letícia Harumi; Lopes, Érica Camelo Viana; Filho, Jomal Rodrigues Barbosa; Ulhôa, Cirano José; Georg, Raphaela de Castro

doi:10.1016/j.micres.2019.126296

Cited by 17 publications

(6 citation statements)

References 45 publications

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“…Most stressor response-related genes that were previously reported to respond to temperature change did not exhibit significant differences in expression levels in this study. For example, heat shock proteins (HSP) are involved in the folding and unfolding of proteins, and play an integral role in the response to temperature and other stressors ( Mota et al, 2019 ). However, in our study, HSP genes did not change significantly ( Supplementary Figure 5 ).…”

Section: Discussionmentioning

confidence: 99%

Transcriptional differences between major Fusarium pathogens of maize, Fusarium verticillioides and Fusarium graminearum with different optimum growth temperatures

et al. 2022

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Fusarium verticillioides and Fusarium graminearum are important pathogens causing disease in maize (Zea mays) worldwide. The distributions of these fungal pathogens vary greatly in different regions and in different years, and are influenced by environmental and climatic conditions. Temperature has significant effects on the growth and mycotoxin production of Fusarium species. In this study, the effects of temperature on the growth and pathogenicity of F. verticillioides and F. graminearum were investigated. F. verticillioides grew fastest and exhibited the strongest pathogenicity to maize stems and grains at 30°C, while F. graminearum grew best at 20°C. Both species produced more toxins at 20°C than at 30°C. To explain the interspecific differences in the relationship of growth and temperature, RNA-seq was used to compare F. verticillioides and F. graminearum cultivated for 4 d at the optimum temperatures of 30°C and 20°C, respectively. Samples of F. verticillioides were also cultivated for 9 d (to maximize toxin production) at 20°C and 30°C and analyzed by RNA-seq to investigate the influence of temperature for different growth stages. The differently expressed genes (DEGs) were identified by comparison of cultures grown for the same amount of time but at different temperatures. GO enrichment analysis showed high enrichment of DEGs in categories of membrane part, catalytic activity, metabolic process, and growth at warmer temperature resulted in more down-regulated DEGs enriched in membrane components in all groups. KEGG analysis revealed enrichment of DEGs related to different temperatures in carbohydrate and amino acid metabolism pathways. For both species, there was decreased expression of many DEGs related to amino acid metabolism when cultivated at warm temperature, such as genes related to beta-alanine metabolism and arginine and proline metabolism. However, changes in genes related to glyoxylate and dicarboxylate metabolism and fatty acid degradation were more related to the growth state. The results showing different responses pattern of these pathways provides a foundation for further investigation of the molecular mechanisms underlying distinct thermal ecological niches of F. verticillioides and F. graminearum.

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Section: Discussionmentioning

confidence: 99%

Transcriptional differences between major Fusarium pathogens of maize, Fusarium verticillioides and Fusarium graminearum with different optimum growth temperatures

et al. 2022

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“…Through our analysis, we reported for the first time the participation and contribution of cell wall degrading enzymes with specific function against vector of diseases. T. asperellum CWDEs are inducible by different substrates, high temperature enhances their activity and mycoparasitism genes are differentially expressed and regulated in self‐recognition 12,39–43 …”

Section: Discussionmentioning

confidence: 99%

“…T. asperellum CWDEs are inducible by different substrates, high temperature enhances their activity and mycoparasitism genes are differentially expressed and regulated in self-recognition. 12,[39][40][41][42][43] Subsequent efforts will be focused on understanding the gene expression profile of T. asperellum while exposed to different chitin sources.…”

Section: Analysis Of Cuticle Degradation Of the A Aegypti L3 Larvae B...mentioning

confidence: 99%

Larvicidal potential of cell wall degrading enzymes from Trichoderma asperellum against Aedes aegypti (Diptera: Culicidae)

Silveira

Andrade

Guissoni

et al. 2021

Biotechnology Progress

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Aedes aegypti is a mosquito vector of arboviruses such as dengue, chikungunya, zika and yellow fever that cause important public health diseases. The incidence and gravity of these diseases justifies the search for effective measures to reduce the presence of this vector in the environment. Bioinsecticides are an effective alternative method for insect control, with added ecological benefits such as biodegradability. The current study demonstrates that a chitinolytic enzyme complex produced by the fungus Trichoderma asperellum can disrupt cuticle formation in the L3 larvae phase of A. aegypti, suggesting such biolarvicidal action could be used for mosquito control. T. asperellum was exposed to chitin from different sources. This induction of cell wall degrading enzymes, including chitinase, N‐acetylglucosaminidase and β‐1,3‐glucanase. Groups of 20 L3 larvae of A. aegypti were exposed to varying concentrations of chitinolytic enzymes induced with commercial chitin (CWDE) and larvae cell wall degrading enzymes (L‐CWDE). After 72 h of exposure to the CWDE, 100% of larvae were killed. The same percent mortality was observed after 48 h of exposure to L‐CWDE at half the CWDE enzyme mixture concentration. Exoskeleton deterioration was further observed by scanning and electron microscopy. Our findings indicate that L‐CWDE produced by T. asperellum reflect chitinolytic enzymes with greater specificity for L3 larval biomolecules. This specificity is characterized by the high percentage of mortality compared with CWDE treatments and also by abrupt changes in patterns of the cellular structures visualized by scanning and transmission electron microscopy. These mixtures of chitinolytic enzymes could be candidates, as adjuvant or synergistic molecules, to replace conventional chemical insecticides currently in use.

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“…Beneficial microorganisms, such as Pseudomonas and Trichoderma , can enhance the expression of heat shock proteins (HSPs), which help maintain protein integrity [ 84 , 85 ]. Microorganisms that produce HSPs can interact directly with plant organs, from seeds and seedlings to mature plants.…”

Section: Metabolic Responses Of Plants and Their Associated Microorga...mentioning

confidence: 99%

Juvenile Plant–Microbe Interactions Modulate the Adaptation and Response of Forest Seedlings to Rapid Climate Change

Sanhueza,

Hernández,

Sagredo-Sáez

et al. 2024

Plants

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The negative impacts of climate change on native forest ecosystems have created challenging conditions for the sustainability of natural forest regeneration. These challenges arise primarily from abiotic stresses that affect the early stages of forest tree development. While there is extensive evidence on the diversity of juvenile microbial symbioses in agricultural and fruit crops, there is a notable lack of reports on native forest plants. This review aims to summarize the critical studies conducted on the diversity of juvenile plant–microbe interactions in forest plants and to highlight the main benefits of beneficial microorganisms in overcoming environmental stresses such as drought, high and low temperatures, metal(loid) toxicity, nutrient deficiency, and salinity. The reviewed studies have consistently demonstrated the positive effects of juvenile plant–microbiota interactions and have highlighted the potential beneficial attributes to improve plantlet development. In addition, this review discusses the beneficial attributes of managing juvenile plant–microbiota symbiosis in the context of native forest restoration, including its impact on plant responses to phytopathogens, promotion of nutrient uptake, facilitation of seedling adaptation, resource exchange through shared hyphal networks, stimulation of native soil microbial communities, and modulation of gene and protein expression to enhance adaptation to adverse environmental conditions.

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Hsp genes are differentially expressed during Trichoderma asperellum self-recognition, mycoparasitism and thermal stress

Cited by 17 publications

References 45 publications

Transcriptional differences between major Fusarium pathogens of maize, Fusarium verticillioides and Fusarium graminearum with different optimum growth temperatures

Transcriptional differences between major Fusarium pathogens of maize, Fusarium verticillioides and Fusarium graminearum with different optimum growth temperatures

Larvicidal potential of cell wall degrading enzymes from Trichoderma asperellum against Aedes aegypti (Diptera: Culicidae)

Juvenile Plant–Microbe Interactions Modulate the Adaptation and Response of Forest Seedlings to Rapid Climate Change

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