Hsp110 expression changes in rat primary myocardial cells exposed to heat stress in vitro

Liu, Z J; Lv, Yuan; Zhang, M; Yue, Zhenming; Tang, Shu; Islam, Aminul; Rehana, B; Bao, Endong; Hartung, J.

doi:10.4238/2012.november.29.1

Cited by 9 publications

(5 citation statements)

References 39 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Cardio-specific marker enzymes have been shown to be released from the heart into the blood stream during myocardial damage because of degeneration of myofibrils and myocytes (Senthil et al, 2007). An in vitro study showed significantly increased enzymes such as AST, LDH, and CK in the culture medium of primary rat myocardial cells, suggesting that the integrity of these cells was altered because of heat stress (Liu et al, 2012). Our results showed that diagnostic marker enzymes clearly were elevated.…”

Section: Discussionmentioning

confidence: 50%

Evaluation of Hsp47 expression in heat-stressed rat myocardial cells in vitro and in vivo

Abdelnasir¹,

Sun²,

Cheng³

et al. 2014

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

ABSTRACT. The aim of the present study was to identify the correlation between expression of heat shock protein 47 (Hsp47) and stress injury in heat-stressed myocardial cells and to compare variations in Hsp47 expression in rat myocardial cells exposed to different heat stress for varying periods in vitro and in vivo. Exposure to heat stress at 42°C resulted in similar induction patterns of the heart damage-related enzyme aspartate aminotransferase in the supernatants of H9c2 cells and in the serum of rats. Histological analysis revealed that both H9c2 cells and heart tissues displayed cellular degeneration in response to different periods of heat stress. Hsp47 was constitutively expressed in the cytoplasm of H9c2 cells at all time points during heat stress, which was consistent with observations in heart fibers in vivo. Immunoblotting analysis revealed no significant difference between the expression of Hsp47 in H9c2 cells and heart tissue. However, the expression of hsp47 mRNA in response to heat stress was significantly increased in H9c2 cells at 60 min (P < 0.01) and 100 min (P < 0.01), which was comparable to that at 100 min (P < 0.01) in the rat heart. Thus, Hsp47 was elevated significantly after hyperthermia at the mRNA level but not at the protein level both in vitro and in vivo. The results suggest that Hsp47 turnover may increase during heat stress or that Hsp47 consumption exceeds its production.

show abstract

Section: Discussionmentioning

confidence: 50%

Evaluation of Hsp47 expression in heat-stressed rat myocardial cells in vitro and in vivo

Abdelnasir¹,

Sun²,

Cheng³

et al. 2014

Genet. Mol. Res.

Self Cite

View full text Add to dashboard Cite

show abstract

“…In addition, heat shock protein A4 ( HSPA4 ), a member of the HSP110 family, can control apoptosis of inflammatory cells and immune reactions in the gut [ 52 ]. In Liu et al’s study, it was found that the expression of Hsp110 in primary cardiomyocytes in vitro was sensitive to heat stress, and Hsp110 participated in the potential acquisition of heat tolerance after heat stress [ 53 ]. Therefore, these genes may be related to heat tolerance in Chinese local pigs, providing guidance for further exploration of heat tolerance genes.…”

Section: Discussionmentioning

confidence: 99%

Evaluation of the Genetic Diversity, Population Structure and Selection Signatures of Three Native Chinese Pig Populations

Zhong

Wang

Xie

et al. 2023

Animals

View full text Add to dashboard Cite

Indigenous pig populations in Hainan Province live in tropical climate conditions and a relatively closed geographical environment, which has contributed to the formation of some excellent characteristics, such as heat tolerance, strong disease resistance and excellent meat quality. Over the past few decades, the number of these pig populations has decreased sharply, largely due to a decrease in growth rate and poor lean meat percentage. For effective conservation of these genetic resources (such as heat tolerance, meat quality and disease resistance), the whole-genome sequencing data of 78 individuals from 3 native Chinese pig populations, including Wuzhishan (WZS), Tunchang (TC) and Dingan (DA), were obtained using a 150 bp paired-end platform, and 25 individuals from two foreign breeds, including Landrace (LR) and Large White (LW), were downloaded from a public database. A total of 28,384,282 SNPs were identified, of which 27,134,233 SNPs were identified in native Chinese pig populations. Both genetic diversity statistics and linkage disequilibrium (LD) analysis indicated that indigenous pig populations displayed high genetic diversity. The result of population structure implied the uniqueness of each native Chinese pig population. The selection signatures were detected between indigenous pig populations and foreign breeds by using the population differentiation index (FST) method. A total of 359 candidate genes were identified, and some genes may affect characteristics such as immunity (IL-2, IL-21 and ZFYVE16), adaptability (APBA1), reproduction (FGF2, RNF17, ADAD1 and HIPK4), meat quality (ABCA1, ADIG, TLE4 and IRX5), and heat tolerance (VPS13A, HSPA4). Overall, the findings of this study will provide some valuable insights for the future breeding, conservation and utilization of these three Chinese indigenous pig populations.

show abstract

“…Furthermore, the mitochondrial membrane potential and the intensity of calcium fluorescence, which were testing indexes in the present study, play an essential role in the mitochondrial function of myocardial tissue homeostasis ( Skarka and Ostadal, 2002 ; Dibb et al, 2007 ; Kadenbach et al, 2011 ; Davlouros et al, 2016 ; Zorova et al, 2018 ; Schartner et al, 2019 ; Lai and Qiu, 2020 ). Understanding of the electrophysiological effects in cardiomyocyte contractile and mechanical function in response to cardiotoxic drugs has previously relied on primary cardiomyocytes from animal models ( Liu et al, 2012 ; Tang et al, 2016 ; Blair and Pruitt, 2020 ). Therefore, this research selected the abovementioned indexes to quantify the myocardial cytotoxicity of evodiamine and rutaecarpine in an effort to understand how these bioactive compounds of Euodiae Fructus directly impact the cGMP-PKG pathway at the cellular and cardiomyocytes levels.…”

Section: Discussionmentioning

confidence: 99%

Potential cardiotoxicity induced by Euodiae Fructus: In vivo and in vitro experiments and untargeted metabolomics research

Zhang

Lü²,

Ren³

et al. 2022

Front. Pharmacol.

View full text Add to dashboard Cite

Background: Euodiae Fructus, a well-known herbal medicine, is widely used in Asia and has also gained in popularity in Western countries over the last decades. It has known side effects, which have been observed in clinical settings, but few studies have reported on its cardiotoxicity.Methods: In the present study, experiments using techniques of untargeted metabolomics clarify the hazardous effects of Euodiae Fructus on cardiac function and metabolism in rats in situations of overdosage and unsuitable syndrome differentiation. In vitro assays are conducted to observe the toxic effects of evodiamine and rutaecarpine, two main chemical constituents of Euodiae Fructus, in H9c2 and neonatal rat cardiomyocytes (NRCMs), with their signaling mechanisms analyzed accordingly.Results: The cardiac cytotoxicity of evodiamine and rutaecarpine in in vivo experiments is associated with remarkable alterations in lactate dehydrogenase, creatine kinase, and mitochondrial membrane potential; also with increased intensity of calcium fluorescence, decreased protein expression of the cGMP-PKG pathway in H9c2 cells, and frequency of spontaneous beat in NRCMs. Additionally, the results in rats with Yin deficiency receiving a high-dosage of Euodiae Fructus suggest obvious cardiac physiological dysfunction, abnormal electrocardiogram, pathological injuries, and decreased expression of PKG protein. At the level of endogenous metabolites, the cardiac side effects of overdose and irrational usage of Euodiae Fructus relate to 34 differential metabolites and 10 metabolic pathways involving among others, the purine metabolism, the glycerophospholipid metabolism, the glycerolipid metabolism, and the sphingolipid metabolism.Conclusion: These findings shed new light on the cardiotoxicity induced by Euodiae Fructus, which might be associated with overdose and unsuitable syndrome differentiation, that comes from modulating the cGMP-PKG pathway and disturbing the metabolic pathways of purine, lipid, and amino acid. Continuing research is needed to ensure pharmacovigilance for the safe administration of Chinese herbs in the future.

show abstract

Hsp110 expression changes in rat primary myocardial cells exposed to heat stress in vitro

Cited by 9 publications

References 39 publications

Evaluation of Hsp47 expression in heat-stressed rat myocardial cells in vitro and in vivo

Evaluation of Hsp47 expression in heat-stressed rat myocardial cells in vitro and in vivo

Evaluation of the Genetic Diversity, Population Structure and Selection Signatures of Three Native Chinese Pig Populations

Potential cardiotoxicity induced by Euodiae Fructus: In vivo and in vitro experiments and untargeted metabolomics research

Contact Info

Product

Resources

About