HSV vector-mediated transduction and GDNF secretion from adipose cells

Fradette, Julie; Wolfe, Darren; Goins, William F.; Huang, Shaohua; Flanigan, R M

doi:10.1038/sj.gt.3302359

Cited by 12 publications

(6 citation statements)

References 39 publications

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“…The glial cell line-derived neurotrophic factor ( GDNF ) is annotated in the 36 Mb genomic region on BTA20. The protein encoded by this gene has been described as secreted from adipose cells [ 40 ]. The adipocytes secrete hormones with an important role in fat deposition and obesity [ 41 ].…”

Section: Resultsmentioning

confidence: 99%

Identification of genomic regions associated with feed efficiency in Nelore cattle

et al. 2014

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BackgroundFeed efficiency is jointly determined by productivity and feed requirements, both of which are economically relevant traits in beef cattle production systems. The objective of this study was to identify genes/QTLs associated with components of feed efficiency in Nelore cattle using Illumina BovineHD BeadChip (770 k SNP) genotypes from 593 Nelore steers. The traits analyzed included: average daily gain (ADG), dry matter intake (DMI), feed-conversion ratio (FCR), feed efficiency (FE), residual feed intake (RFI), maintenance efficiency (ME), efficiency of gain (EG), partial efficiency of growth (PEG) and relative growth rate (RGR). The Bayes B analysis was completed with Gensel software parameterized to fit fewer markers than animals. Genomic windows containing all the SNP loci in each 1 Mb that accounted for more than 1.0% of genetic variance were considered as QTL region. Candidate genes within windows that explained more than 1% of genetic variance were selected by putative function based on DAVID and Gene Ontology.ResultsThirty-six QTL (1-Mb SNP window) were identified on chromosomes 1, 2, 3, 5, 6, 7, 8, 9, 10, 12, 14, 15, 16, 18, 19, 20, 21, 22, 24, 25 and 26 (UMD 3.1). The amount of genetic variance explained by individual QTL windows for feed efficiency traits ranged from 0.5% to 9.07%. Some of these QTL minimally overlapped with previously reported feed efficiency QTL for Bos taurus. The QTL regions described in this study harbor genes with biological functions related to metabolic processes, lipid and protein metabolism, generation of energy and growth. Among the positional candidate genes selected for feed efficiency are: HRH4, ALDH7A1, APOA2, LIN7C, CXADR, ADAM12 and MAP7.ConclusionsSome genomic regions and some positional candidate genes reported in this study have not been previously reported for feed efficiency traits in Bos indicus. Comparison with published results indicates that different QTLs and genes may be involved in the control of feed efficiency traits in this Nelore cattle population, as compared to Bos taurus cattle.Electronic supplementary materialThe online version of this article (doi:10.1186/s12863-014-0100-0) contains supplementary material, which is available to authorized users.

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Section: Resultsmentioning

confidence: 99%

Identification of genomic regions associated with feed efficiency in Nelore cattle

et al. 2014

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“…A high expression of HVEM was detected in human pre-adipocytes and adipocytes in culture from obese subjects. 18 Both HVEM gene expression and protein levels were positively associated with LIGHT concentrations and increased in a dose-dependent manner in cultured differentiated adipocytes. Morel et al provided evidence that HVEM expression was downregulated by LIGHT stimulation on activated T cells.…”

Section: Discussionmentioning

confidence: 98%

“…HVEM has a wide tissue distribution 17 and its expression has been found in different cell types including adipocytes. 17,18 LIGHT-HVEM signalling results in T-cell activation and expansion, and modulates a number of T-cell responses, 19 promoting the development of T-cell-associated autoimmune diseases. 20 This cytokine has also been suggested to promote atherogenesis by inducing matrix metalloproteinase activity and inflammation in macrophages.…”

Section: Introductionmentioning

confidence: 99%

LIGHT is associated with hypertriglyceridemia in obese subjects and increased cytokine secretion from cultured human adipocytes

et al. 2009

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Background: LIGHT (lymphotoxin-like inducible protein that competes with glycoprotein D for herpesvirus entry on T cells) is a member of the tumor necrosis factor (TNF) family, primarily expressed in lymphocytes, which was associated with the induction of pro-inflammatory cytokines and alterations of lipid homeostasis in animal models. We aimed to analyze whether LIGHT has a role in the human obesity-associated inflammatory status. Methods: The association between circulating LIGHT concentrations and clinical variables was studied in 190 subjects with different degrees of obesity and glucose tolerance. The expression and release of 21 different cytokines, and the expression of genes involved in lipid metabolism were also evaluated after stimulation with LIGHT in cultured human differentiated adipocytes. Results: Serum LIGHT concentrations positively associated with body mass index (BMI), fat mass, glycated hemoglobin and fasting triglycerides, and negatively with high-density lipoprotein cholesterol. Circulating LIGHT concentrations were significantly increased in morbidly obese subjects and in patients with type 2 diabetes. LIGHT induced the secretion of several cytokines and upregulated the expression and secretion of interleukin-6 (IL-6), IL-8, Growth Regulated Oncogene (GRO) and monocyte chemotactic protein-1 (MCP-1). These observations were concomitant with the activation of nuclear factor (NF)-kB signalling in human differentiated adipocytes. LIGHT also upregulated the expression and synthesis of its own receptor (herpesvirus entry mediator (HVEM)) and decreased the expression of peroxisome proliferator-activated receptor-g (PPAR-g) and fatty acid synthase. Conclusion: These data suggest that LIGHT may have a role in mediating chronic inflammation and alterations of lipid metabolism in obese subjects.

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“…However, HVEM gene expression was not only present in isolated adipocytes, but was also found to increase during the differentiation process of preadipocytes as they became mature adipocytes of human primary cultures. Fradette et al (12) demonstrated using flow cytometry and indirect immunocytochemistry in their research into HSV‐mediated transduction that HVEM was present on the surface of both human preadipocytes and mature adipocytes.…”

Section: Discussionmentioning

confidence: 99%

Characterization of Herpes Virus Entry Mediator as a Factor Linked to Obesity

Bassols

Moreno

Ortega

et al. 2010

Obesity

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Herpes virus entry mediator (HVEM) is a member of the tumor necrosis factor (TNF) receptor superfamily (TNFRSF14), which serves as a receptor for herpes viruses and cytokines such as lymphotoxin‐α (LT‐α) and LIGHT (lymphotoxin‐like inducible protein that competes with glycoprotein D for herpes virus entry on T cells). We aimed to explore the associations of HVEM with human obesity. HVEM gene expression and protein levels were studied in total adipose tissue and in their fractions (isolated adipocytes and stromovascular cells (SVCs)) obtained from 81 subjects during elective surgical procedures. HVEM −241GA and −14AG gene polymorphisms were also studied and associated with obesity measures in 840 subjects. Visceral adipose tissue had significantly higher expression of HVEM than subcutaneous adipose tissue (P < 0.0001). Obese patients had significantly higher subcutaneous HVEM gene expression (P = 0.03) and protein levels (P = 0.01) than lean subjects. HVEM gene expression and protein levels were found in both isolated adipocytes and SVCs. These findings were confirmed in primary cultures from human preadipocytes, in which a significant increase in HVEM was observed during the differentiation process. HVEM −241GA and −14AG gene polymorphisms were associated with obesity, diastolic pressure, several inflammatory parameters (C‐reactive protein and interleukin 18 (IL‐18)), and circulating LIGHT concentrations. A sample of men with the G241A gene polymorphism also showed an increased serum titer of IgG antiherpes virus 1. These results provide evidences of an existing relationship between HVEM and obesity, which suggest that this TNF superfamily receptor could be involved in the pathogenesis of obesity and inflammation‐related activity.

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HSV vector-mediated transduction and GDNF secretion from adipose cells

Cited by 12 publications

References 39 publications

Identification of genomic regions associated with feed efficiency in Nelore cattle

Identification of genomic regions associated with feed efficiency in Nelore cattle

LIGHT is associated with hypertriglyceridemia in obese subjects and increased cytokine secretion from cultured human adipocytes

Characterization of Herpes Virus Entry Mediator as a Factor Linked to Obesity

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