Human Adipose-Derived and Amniotic Fluid-Derived Stem Cells: A Preliminary In Vitro Study Comparing Myogenic Differentiation Capability

Bajek, Anna; Olkowska, Joanna; Walentowicz-Sadlecka, Małgorzata; Sadłecki, Paweł; Grabiec, Marek; Porowińska, Dorota; Drewa, Tomasz; Roszkowski, Krzysztof

doi:10.12659/msm.905826

Cited by 15 publications

(5 citation statements)

References 57 publications

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“…Similarly, a comparative study revealed that adMSC expressed significantly more cardiomyocyte specific biomarkers as DFSCs following cardiac programming with cytokine supplemented culture medium [11]. The impact of MSC origin on programming capability was also shown for non-cardiac cell lineages like hepatocytes and smooth muscle cells [59,62].…”

Section: Discussionmentioning

confidence: 89%

RNA-Based Strategies for Cardiac Reprogramming of Human Mesenchymal Stromal Cells

Mueller

Wolfien

Ekat

et al. 2020

Cells

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Multipotent adult mesenchymal stromal cells (MSCs) could represent an elegant source for the generation of patient-specific cardiomyocytes needed for regenerative medicine, cardiovascular research, and pharmacological studies. However, the differentiation of adult MSC into a cardiac lineage is challenging compared to embryonic stem cells or induced pluripotent stem cells. Here we used non-integrative methods, including microRNA and mRNA, for cardiac reprogramming of adult MSC derived from bone marrow, dental follicle, and adipose tissue. We found that MSC derived from adipose tissue can partly be reprogrammed into the cardiac lineage by transient overexpression of GATA4, TBX5, MEF2C, and MESP1, while cells isolated from bone marrow, and dental follicle exhibit only weak reprogramming efficiency. qRT-PCR and transcriptomic analysis revealed activation of a cardiac-specific gene program and up-regulation of genes known to promote cardiac development. Although we did not observe the formation of fully mature cardiomyocytes, our data suggests that adult MSC have the capability to acquire a cardiac-like phenotype when treated with mRNA coding for transcription factors that regulate heart development. Yet, further optimization of the reprogramming process is mandatory to increase the reprogramming efficiency.

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Section: Discussionmentioning

confidence: 89%

RNA-Based Strategies for Cardiac Reprogramming of Human Mesenchymal Stromal Cells

Mueller

Wolfien

Ekat

et al. 2020

Cells

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“…Another cell population used in skeletal muscle TE is mesenchymal stem cells (MSCs). MSCs are multipotent progenitor cells that are found in the stroma of various tissue types (e.g., umbilical cord, adipose tissue) and can differentiate into multiple cell lineages of the mesodermal lineage, including adipocytes, osteoblasts, chondrocytes, or myocytes [33][34][35]. The most commonly used tissues for the isolation of MSCs are bone marrow (BMSCs) and adipose tissue (ADSCs).…”

Section: Cellular Aspects Of Skeletal Muscle Tementioning

confidence: 99%

Three-Dimensional Bioprinting in Soft Tissue Engineering for Plastic and Reconstructive Surgery

Bülow,

Schäfer,

Beier

2023

Bioengineering

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Skeletal muscle tissue engineering (TE) and adipose tissue engineering have undergone significant progress in recent years. This review focuses on the key findings in these areas, particularly highlighting the integration of 3D bioprinting techniques to overcome challenges and enhance tissue regeneration. In skeletal muscle TE, 3D bioprinting enables the precise replication of muscle architecture. This addresses the need for the parallel alignment of cells and proper innervation. Satellite cells (SCs) and mesenchymal stem cells (MSCs) have been utilized, along with co-cultivation strategies for vascularization and innervation. Therefore, various printing methods and materials, including decellularized extracellular matrix (dECM), have been explored. Similarly, in adipose tissue engineering, 3D bioprinting has been employed to overcome the challenge of vascularization; addressing this challenge is vital for graft survival. Decellularized adipose tissue and biomimetic scaffolds have been used as biological inks, along with adipose-derived stem cells (ADSCs), to enhance graft survival. The integration of dECM and alginate bioinks has demonstrated improved adipocyte maturation and differentiation. These findings highlight the potential of 3D bioprinting techniques in skeletal muscle and adipose tissue engineering. By integrating specific cell types, biomaterials, and printing methods, significant progress has been made in tissue regeneration. However, challenges such as fabricating larger constructs, translating findings to human models, and obtaining regulatory approvals for cellular therapies remain to be addressed. Nonetheless, these advancements underscore the transformative impact of 3D bioprinting in tissue engineering research and its potential for future clinical applications.

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“…Moreover, these cells exhibited properties of MSCs, such as multilineage differentiation potential towards fibroblasts, adipocytes, osteocytes [ 265 ], and chondrogenic lineage [ 266 ]. Ovine mesenchymal amniocytes were shown to give rise to smooth and skeletal muscle cells after the treatment with promyogenic medium, which resulted in expression of transgelin, calponin, and α-actin [ 267 ].…”

Section: Signaling Pathways Involved In Fetal-derived Msc Development and Differentiationmentioning

confidence: 99%

Mesenchymal Stem/Stromal Cells Derived from Human and Animal Perinatal Tissues—Origins, Characteristics, Signaling Pathways, and Clinical Trials

Kulus

Sibiak

Stefańska

et al. 2021

Cells

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Mesenchymal stem/stromal cells (MSCs) are currently one of the most extensively researched fields due to their promising opportunity for use in regenerative medicine. There are many sources of MSCs, of which cells of perinatal origin appear to be an invaluable pool. Compared to embryonic stem cells, they are devoid of ethical conflicts because they are derived from tissues surrounding the fetus and can be safely recovered from medical waste after delivery. Additionally, perinatal MSCs exhibit better self-renewal and differentiation properties than those derived from adult tissues. It is important to consider the anatomy of perinatal tissues and the general description of MSCs, including their isolation, differentiation, and characterization of different types of perinatal MSCs from both animals and humans (placenta, umbilical cord, amniotic fluid). Ultimately, signaling pathways are essential to consider regarding the clinical applications of MSCs. It is important to consider the origin of these cells, referring to the anatomical structure of the organs of origin, when describing the general and specific characteristics of the different types of MSCs as well as the pathways involved in differentiation.

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Human Adipose-Derived and Amniotic Fluid-Derived Stem Cells: A Preliminary In Vitro Study Comparing Myogenic Differentiation Capability

Cited by 15 publications

References 57 publications

RNA-Based Strategies for Cardiac Reprogramming of Human Mesenchymal Stromal Cells

RNA-Based Strategies for Cardiac Reprogramming of Human Mesenchymal Stromal Cells

Three-Dimensional Bioprinting in Soft Tissue Engineering for Plastic and Reconstructive Surgery

Mesenchymal Stem/Stromal Cells Derived from Human and Animal Perinatal Tissues—Origins, Characteristics, Signaling Pathways, and Clinical Trials

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