Human Adipose Stem Cells in Collagen on Angiogenesis Process of Burn Healing in Rat Model: Its Number of Blood Vessels

Abstract

Angiogenesis in the burn wound healing process is important. The application stem cell therapy in burn wound problem is expected to increase formation of new blood vessel so that the distribution of oxygen and nutrients working well. The purpose of this study was to known effect of human Adipose Derived Stem Cell (hADSC) in collagen gel on the average number of blood vessels based on time dependent. This study used 25 male rats Sprague Dawley who had received three internal wounds with different treatments. The treatments used hADSC in collagen gel, collagen gel only and without treatment. The rats were divided into 5 groups based on the duration of observation day 0, 7, 14, 21 and 28. Rat without burn wound were used as control. Observation blood vessels number using hematoxylin-eosin staining and calculated of average number blood vessels from five filed in five mice. Statistical analysis used ANOVA one way with significance value p<0.05. The average number blood vessels increase significantly until day 21 and decreased on day 28, both in untreated burn wound, collagen gel and hADSC in collagen gel. The treatment with hADSC in collagen gel was not significantly different from the collagen gel group or without treatment at day 7, 14 and 21. Even though the average of blood vessels of treatment with hADSC in collagen gel was not significantly different but our research had showed that treatment with hADSC in collagen gel was higher number of blood vessels than another group. Decrease of blood vessel number at 28 days indicated start of near homeostasis state and treatment hADSC in collagen gel has significantly decreased than another group. Our research showed that angiogenesis was time dependent and hADSC in collagen gel have not a role in increase of number blood vessels but we have indicated that hADSC might be have a role in qualitative improvement of angiogenesis.

Emulgel based on fish skin collagen‐microalgae‐silver increased neovascularization and re‐epithelialization of full thickness burn in rats

“…Moreover, previous studies have reported that colloidal solutions of silver nanoparticles and silver sulfadiazine cream already were related to increase the neovascularization and re‐epithelization of burns in rats 68 . Finally, the restoration of the vascular network allows the transport of oxygen, nutrients, and immune cells to the injured area, which are essential events for the progression of the healing process 69 …”

“…68 Finally, the restoration of the vascular network allows the transport of oxygen, nutrients, and immune cells to the injured area, which are essential events for the progression of the healing process. 69 The emulgels' similar biological behavior could be attributed to the components of COL, GEL, and sunflower oil. Sunflower oil has been reported to promote wound healing by stimulating the processes of neovascularization, fibroblast migration, and proliferation.…”

Application of Human Adipose Stem Cell (hADSC) in Collagen Gel on Rat Deep Dermal Burn

“…To develop deep dermal burns, rats were anesthesized and shaved in dorsal section. An iron plate of 1.2 cm in diameter with a temperature of 250C was pressed on shaved skin for 15 seconds (Sari et al, 2018;Yunaini et al, 2019). Rats were saccrificed on day-7, -14, -21 and -28 and burn tissues were taken for histological examinations.…”

“…The cultures were observed every day. Medium change was done every 2-3 days and harvested was done after 80% confluent Yunaini et al, 2019). (Fig.…”

“…Tissue samples were sectioned with microtome and were stained with Masson's Trichrome to assess collagen density. 1st Wound without treatment, 2nd wound was given collagen gel and 3th wound was given human ADSCs in collagen gel (Sari et al, 2018;Yunaini et al, 2019) Masson's Trichrome Procedure Deparafinization was done by soaking the slides in xylol 1, xylol II, xylol III for 10 minutes. The process was continued with rehydration, in Alcohol 100%, alcohol 95%, alcohol 70%, finally in distilled water.…”