Human adipose tissue-derived mesenchymal stem cells in rheumatoid arthritis: Regulatory effects on peripheral blood mononuclear cells activation

Baharlou, Rasoul; Ahmadi-Vasmehjani, Abbas; Faraji, Fatemeh; Atashzar, Mohammad Reza; Khoubyari, Mahshid; Ahi, Salma; Erfanian, Saiedeh; Navabi, Shadi-Sadat

doi:10.1016/j.intimp.2017.03.016

Cited by 47 publications

(27 citation statements)

References 36 publications

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“…In injured sites, ADSCs were recruited and were migrated by increasing their expression of CXCR-4 molecules, which led to modulating the inflammatory phenotype of local immune cells to a healing anti-inflammatory one. Their presence was critical to drive the inflammatory profile of macrophages, T, B, and dendritic cells initiating, and thus the proliferation and remodeling phases of wound healing [51,52]. Their action was likely activated by TGF-β or GDF11 or both of them [53].…”

Section: Role Of the Microenvironment In Adscs Inductionmentioning

confidence: 99%

Hopes and Limits of Adipose-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells (MSCs) in Wound Healing

Mazini

Rochette

Admou

et al. 2020

IJMS

340

219

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Adipose tissue derived stem cells (ADSCs) are mesenchymal stem cells identified within subcutaneous tissue at the base of the hair follicle (dermal papilla cells), in the dermal sheets (dermal sheet cells), in interfollicular dermis, and in the hypodermis tissue. These cells are expected to play a major role in regulating skin regeneration and aging-associated morphologic disgraces and structural deficits. ADSCs are known to proliferate and differentiate into skin cells to repair damaged or dead cells, but also act by an autocrine and paracrine pathway to activate cell regeneration and the healing process. During wound healing, ADSCs have a great ability in migration to be recruited rapidly into wounded sites added to their differentiation towards dermal fibroblasts (DF), endothelial cells, and keratinocytes. Additionally, ADSCs and DFs are the major sources of the extracellular matrix (ECM) proteins involved in maintaining skin structure and function. Their interactions with skin cells are involved in regulating skin homeostasis and during healing. The evidence suggests that their secretomes ensure: (i) The change in macrophages inflammatory phenotype implicated in the inflammatory phase, (ii) the formation of new blood vessels, thus promoting angiogenesis by increasing endothelial cell differentiation and cell migration, and (iii) the formation of granulation tissues, skin cells, and ECM production, whereby proliferation and remodeling phases occur. These characteristics would be beneficial to therapeutic strategies in wound healing and skin aging and have driven more insights in many clinical investigations. Additionally, it was recently presented as the tool key in the new free-cell therapy in regenerative medicine. Nevertheless, ADSCs fulfill the general accepted criteria for cell-based therapies, but still need further investigations into their efficiency, taking into consideration the host-environment and patient-associated factors.

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Section: Role Of the Microenvironment In Adscs Inductionmentioning

confidence: 99%

Hopes and Limits of Adipose-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells (MSCs) in Wound Healing

Mazini

Rochette

Admou

et al. 2020

IJMS

340

219

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“…PBMCs and Ad-MSCs were obtained according to the previous report. 5 MSC-PBMC co-cultures were prepared using different stimulation regimens in DMEM+10% FBS+antibiotics. Four experimental groups were considered for both patients and healthy groups.…”

Section: Co-culture Of Pbmcs and Ad-mscsmentioning

confidence: 99%

“…4 Although RA is generally considered to depend on IFN-γ-producing Th1 cells, recent studies have indicated an important role for Th17 cells in RA development. 5 Natural Treg cells (Foxp3 + CD4 + CD25 + regulatory T-cell subset) with impaired regulatory function have been detected in RA patients' synovium, especially in the synovial fluid. 6 Th1, Th2, Th17, and Treg cells express T-box 21(Tbet), GATA-binding protein-3 (GATA3), retinoidrelated orphan receptor γt (ROR-γt) and forkhead box P3 (Foxp3), respectively.…”

Section: Introductionmentioning

confidence: 99%

Immunomodulatory Effects of Human Adipose Tissue-derived Mesenchymal Stem Cells on T Cell Subsets in Patients with Rheumatoid Arthritis

Baharlou

Rashidi

Ahmadi-Vasmehjani

et al. 2019

IJAAI

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Adipose-derived mesenchymal stem cells (Ad-MSCs) have been reported to suppress the effector T cell responses and have beneficial effects on various immune disorders, like rheumatoid arthritis (RA). This study was designed to investigate the effects of co-cultured Ad-MSCs on peripheral blood mononuclear cells (PBMCs) of RA patients and healthy individuals, through assessing transcription factors of T cell subsets. PBMCs from RA patients and healthy donors were co-cultured with Ad-MSCs with or without Phytohaemagglutinin (PHA). The quantitative real-time polymerase chain reaction (qRT-PCR) was used to measure the expression of T-box 21 (T-bet), GATA-binding protein-3 (GATA3), retinoid-related orphan receptor γt (ROR-γt) and forkhead box P3 (Foxp3). Based on the results, Ad-MSCs greatly upregulated Th2 and Treg cell transcription factors, i.e., GATA3 and Foxp3 (p<0.05), and downregulated Th1 and Th17 transcription factors, i.e., T-bet and RORγt (p<0.05). These results demonstrate that Ad-MSCs can result in an immunosuppressive environment through inhibition of pro-inflammatory T cells and induction of T cells with a regulatory phenotype. Therefore, they might have important clinical implications for inflammatory and autoimmune diseases such as RA.

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“…In terms of effects on the inflammatory processes, it is well known that ASCs may influence the functional characteristics and cytokine profile of T-, B-, and dendritic cells [54,55,56]. Notably, ASCs have also been shown to be able to induce a conversion of the macrophage phenotype from the pro-inflammatory M1 associated with chronic wounds to the anti-inflammatory and wound healing M2 phenotype [57,58].…”

Section: Using Adipose Stem Cells To Treat Chronic Woundsmentioning

confidence: 99%

Implications of Extracellular Matrix Production by Adipose Tissue-Derived Stem Cells for Development of Wound Healing Therapies

Hyldig

Porsborg

Pennisi

et al. 2017

IJMS

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The synthesis and deposition of extracellular matrix (ECM) plays an important role in the healing of acute and chronic wounds. Consequently, the use of ECM as treatment for chronic wounds has been of special interest—both in terms of inducing ECM production by resident cells and applying ex vivo produced ECM. For these purposes, using adipose tissue-derived stem cells (ASCs) could be of use. ASCs are recognized to promote wound healing of otherwise chronic wounds, possibly through the reduction of inflammation, induction of angiogenesis, and promotion of fibroblast and keratinocyte growth. However, little is known regarding the importance of ASC-produced ECM for wound healing. In this review, we describe the importance of ECM for wound healing, and how ECM production by ASCs may be exploited in developing new therapies for the treatment of chronic wounds.

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Human adipose tissue-derived mesenchymal stem cells in rheumatoid arthritis: Regulatory effects on peripheral blood mononuclear cells activation

Abstract: This study demonstrates that human Ad-MSCs act as key regulators of immune tolerance by inhibiting the inflammation. Therefore, they can be attractive candidates for immunomodulatory cell-based therapy in RA.

Cited by 47 publications

References 36 publications

Hopes and Limits of Adipose-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells (MSCs) in Wound Healing

Hopes and Limits of Adipose-Derived Stem Cells (ADSCs) and Mesenchymal Stem Cells (MSCs) in Wound Healing

Immunomodulatory Effects of Human Adipose Tissue-derived Mesenchymal Stem Cells on T Cell Subsets in Patients with Rheumatoid Arthritis

Implications of Extracellular Matrix Production by Adipose Tissue-Derived Stem Cells for Development of Wound Healing Therapies

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