Human amniotic fluid-based exposure levels of phthalates and bisphenol A mixture reduce INSL3/RXFP2 signaling

Suteau, Valentine; Briet, Claire; Lebeault, M.; Gourdin, L.; Henrion, Didier; Rodien, Patrice; Munier, Mathilde

doi:10.1016/j.envint.2020.105585

Cited by 28 publications

(11 citation statements)

References 87 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…. Previous studies Suteau et al, 2020) have indicated chemicals interaction and suggested an additive effect when applied in a mixture, which is why Kortenkamp and his colleague (Kortenkamp & Koch, 2020) have recommended, in their study, to reduce the Hazard Index for phthalate mixture exposure. Previous literature data (Akash et al, 2020;Castro-Correia et al, 2018) have reported the entanglement of BPA and PAEs in the development of DM as an environmental factor, based on its multifactorial pathogenesis as declared by Lloyd and his colleagues (Lloyd et al, 2021).…”

mentioning

confidence: 99%

Apoptotic susceptibility of pancreatic alpha cells to environmentally relevant dose levels of bisphenol‐A versus dibutyl phthalate is mediated by HSP60/caspase‐3 expression in male albino rats

Morsi

Mersal

Alsabih

et al. 2022

Cell Biology International

View full text Add to dashboard Cite

Unfortunately, humanity is exposed to mixed plasticizers such as bisphenol-A (BPA) and dibutyl phthalate (DBP) that are leached from the daily used plastic products.Previous studies have demonstrated their potential in pancreatic beta cell injury and diabetes induction. The study hypothesized that both compounds would affect the pancreatic alpha cells in albino rats when administered at environmentally relevant doses. Heat shock protein 60 (HSP60) and caspase-3 protein expression was also investigated as potential mechanisms. Thirty-six male Wistar albino rats were separated into four equal groups: control, BPA alone, DBP alone, and BPA + DBP combined groups. BPA and DBP were given in drinking water for 45 days in a dose of 4.5 and 0.8 µg/L, respectively. Fasting blood glucose, serum insulin, pancreatic tissue levels of malondialdehyde, and superoxide dismutase were measured. Pancreatic sections were subjected to hematoxylin & eosin (H & E) staining, glucagon, HSP60, and caspase-3 immunohistochemistry. Although all three experimental groups showed diffuse islet cell HSP60 immunoreactivity, rats exposed to BPA alone showed α-cell-only apoptosis, indicated by H & E changes and caspase-3 immunoreactivity, associated with reduced glucagon immunoreaction.However, rats exposed to DBP alone showed no changes in either α or β-cells. Both combined-exposed animals displayed α and β apoptotic changes associated with islet atrophy and reduced glucagon expression. In conclusion, the study suggested HSP60/caspase-3 interaction, caspase-3 activation, and initiation of apoptosis in αcell only for BPA-alone exposure group, meanwhile DBP alone did not progress to apoptosis. Interestingly, both α/β cell effect was observed in the mixed group implying synergetic/additive action of both chemicals when combined.

show abstract

mentioning

confidence: 99%

Apoptotic susceptibility of pancreatic alpha cells to environmentally relevant dose levels of bisphenol‐A versus dibutyl phthalate is mediated by HSP60/caspase‐3 expression in male albino rats

Morsi

Mersal

Alsabih

et al. 2022

Cell Biology International

View full text Add to dashboard Cite

show abstract

“…Only one study has very recently focused on the effect if EDCs on receptor signaling to INSL3: RXFP2. In a cellular model of HEK293 transiently expressing human RXFP2, individually, BPA, DEHP and DBP potentiate the cAMP response to INSL3 [43]. Because of their ubiquity, BPA, DEHP and DBP are present in many human biological fluids, as the amniotic liquid.…”

Section: Insl3 Receptor Rxfp2mentioning

confidence: 99%

G-Protein Coupled Hormone Receptors of the Hypothalamic-Pituitary-Gonadal Axis are Targets of Endocrine Disrupting Chemicals

Suteau¹,

Rodien²,

Munier³

2021

Hot Topics in Endocrinology and Metabolism

Self Cite

View full text Add to dashboard Cite

Endocrine-disrupting chemicals have received significant concern, since they ubiquitously persist in the environment and are able to induce adverse effects on health, and more particularly on reproductive function. Most of the studies focused on nuclear hormone receptors as mediators of sex steroid hormones signaling. However, there are increasing evidences that peptides hormones of the Hypothalamo-Pituitary-Gonadal axis are targets of endocrine-disrupting chemicals (as Gonadotropin-Releasing Hormone, Follicle-Stimulating Hormone, Luteinizing Hormone…). The majority of these hormones act on G protein-coupled membrane receptors. This review summarizes the effects of endocrine-disrupting chemicals on homeostasis of peptides hormone of Hypothalamo-Pituitary-Gonadal axis and on their G protein-coupled membrane receptors signaling revealed by experimental, clinical, and epidemiological studies in human.

show abstract

“…Hence, people are simultaneously exposed to not merely phthalates, but also other EDCs [11], that is why the Hazard Index for phthalate mixture exposure has been recommended to be reduced [12]. Several studies [13,14] have indicated that these compounds can exert an additive effect when applied in a mixture.…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of Phthalate esters and Bisphenol-A residues in wastewater treatment plants outflow in Saudi Arabia: gas chromatography/mass spectrometry-based analytical study

Mersal

Morsi²,

Alakabawy³

et al. 2023

Egypt. J. Chem.

View full text Add to dashboard Cite

The aquatic system is unfortunately vulnerable to contamination by chemicals as bisphenol A (BPA) and phthalate esters (PAEs) that arise from plastic products frequently used. Water supply is limited in Saudi Arabia. Therefore, many wastewater-treatment plants (WTPs) have been established for wastewater recycling in industry and agricultural purposes. The current study updated and validated the concentrations of BPA and six PAEs (dibutyl phthalate (DBP), dimethyl phthalate (DMP), diethyl phthalate (DEP), butyl benzyl phthalate (BBP), bis (2ethylhexyl) phthalate (DEHP), and dioctyl phthalate (DOP)) in wastewater samples obtained from 5 wastewater treatment plants in 3 Saudi cities. Also, the study compared the concentrations of these chemicals in the secondary WTPs versus the tertiary ones. Chromatography/mass spectrometry was used for the extraction of the chemicals in water samples. All measurements were exposed to comparison and correlation statistical analysis. Both DEHP and DBP were found in all obtained treated wastewater samples. Meanwhile, DOP, BBP, DEP, DMP, and BPA were identified in the following percent of samples, 74.1%, 80.2%, 84%, 84.1%, and 98.01% respectively. The levels of DMP (p ˂ 0.05), DOP, (p ˂ 0.05) and BPA (p ˂ 0.05) were higher in tertiary-treated water sample than those secondary.

show abstract

Human amniotic fluid-based exposure levels of phthalates and bisphenol A mixture reduce INSL3/RXFP2 signaling

Cited by 28 publications

References 87 publications

Apoptotic susceptibility of pancreatic alpha cells to environmentally relevant dose levels of bisphenol‐A versus dibutyl phthalate is mediated by HSP60/caspase‐3 expression in male albino rats

Apoptotic susceptibility of pancreatic alpha cells to environmentally relevant dose levels of bisphenol‐A versus dibutyl phthalate is mediated by HSP60/caspase‐3 expression in male albino rats

G-Protein Coupled Hormone Receptors of the Hypothalamic-Pituitary-Gonadal Axis are Targets of Endocrine Disrupting Chemicals

Quantitative determination of Phthalate esters and Bisphenol-A residues in wastewater treatment plants outflow in Saudi Arabia: gas chromatography/mass spectrometry-based analytical study

Contact Info

Product

Resources

About