Human Amniotic Membrane Extracts have Anti-Inflammatory Effects on Damaged Human Corneal Epithelial Cells <i>In Vitro</i>

Abstract

The human amniotic membrane has therapeutic potential for several diseases such as cardiac ischemia, liver fibrosis, and ocular surface disorders. In the treatment of ocular surface disorders, human amniotic membrane transplantation promotes epithelial wound healing and suppresses inflammation. The objective of this study was to determine whether human amniotic membrane extracts (HAE) help damaged corneal epithelial cells recover from an inflammatory response. Human corneal epithelial cells (hCEC) which were induced inflammation were treated with human amniotic membrane extracts, and then the levels of inflammatory cytokines were measured. Human amniotic membrane extracts had an anti-inflammatory effect on damaged human corneal epithelial cells. More importantly, homogenized human amniotic membrane extracts of less than 3 kDa had a greater capacity for reducing inflammation and secretion of interleukin-6 (IL-6) and interleukin-8 (IL-8). Thus, these results indicate that the use of human amniotic membrane extracts is a promising treatment for ocular surface disorders accompanied by inflammation.

3D-Printed membrane as an alternative to amniotic membrane for ocular surface/conjunctival defect reconstruction: An in vitro & in vivo study

“…The anti-inflammatory mechanism of AM is not fully understood yet. In-vitro studies suggest that AM can inhibit expression of pro-inflammatory cytokines such as interleukin (IL) 1a, IL-1b [69] and IL-8 [70]. It contains immunomodulatory substrates, such as heavy chain (HC)-hyaluronan (HA)/pentraxin 3 (PTX3) in its matrix [21].…”

“…The remnant has been reported to remain 3-10 weeks after cryopreserved AM transplantation in pemphigoid patient's eyes [13] and 9 weeks after transplantation of dry and cryopreserved AM in rabbit eyes [28,29]. On the other hand, the ability to induce apoptosis in inflammatory cells and subsequently reduce inflammation, or other anti-inflammatory mechanisms of AM [20,70,72,73], may compensate for certain types of inflammation to certain degrees. However, it becomes less effective when the inflammation is severe [1,19,21,26].…”

Effect of Lead on Human Middle Ear Epithelial Cells

“…AME, an extract of the human amniotic membrane, has also been shown to have anti-inflammatory effects. From the experiment using human corneal epithelial cells, homogenized human AME less than 3 kDa had a greater capacity to decrease the inflammation and secretion of IL-6 and IL-8 [35]. Human AME has been proven to contain human neutrophil peptides, lysozyme, LL-37 (C-terminal part of the only human cathelicidin), bactericidal/permeability-increasing protein, calprotectin, and ubiquitin, and to show anti-inflammatory effects [36, 37].…”

A Review on Modifications of Amniotic Membrane for Biomedical Applications

“… Lee et al (2016) conducted a study to prove the anti-inflammatory effect of AME on human corneal epithelial cells (hCECs) and showed that it was a more efficient anti-inflammatory agent than negative control without inducing apoptosis. Besides, they attempted to identify which part of AME is responsible for this effect.…”