Human Aorta Cells for Isolation and Propagation of Rhinoviruses.

Isolation from Man of "Avian Infectious Bronchitis Virus-like" Viruses (Coronaviruses) similar to 229E Virus, with Some Epidemiological Observations

“…In the 1965-1967 study of NIH employees, 0.85% NaCI was used as nasal wash fluid, and this fluid was immediately diluted approximately 'h in veal infusion broth with 0.5% bovine albumin. These specimens were inoculated within I hr of collection into 2 roller tubes each of Hep-2, MK, WI26 and/or WI38, human aorta (AT-39), and HEK cultures [15]. The remaining nasal fluid was stored at -60 C. Tissue cultures were obtained from commercial sources, maintained as previously described, incubated at 33-34 C on drums rotating at 12 revolutions per hour, and observed for cytopathic effect (CPE) twice weekly [16].…”

Cultivation of "difficult" viruses from patients with common colds.

“…Our rate of isolating rhinoviruses in tissue culture is lower than that in an earlier study from this laboratory (Kendall et al, 1962) and elsewhere (Hamre et al, 1966), but the numbers are rather small and so the difference is not statistically significant. Phillips et al (1965) recovered rhinoviruses from about a quarter to a half of three series of students with colds by the use of a number of different strains of cells. Some of our specimens containing rhinovirusesfor example, H.G.P.…”