1998
DOI: 10.1016/s0197-4580(98)00076-1
|View full text |Cite
|
Sign up to set email alerts
|

Human apolipoprotein E allele-specific brain expressing transgenic mice

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
3
1
1

Citation Types

4
12
0

Year Published

2000
2000
2010
2010

Publication Types

Select...
7
1

Relationship

0
8

Authors

Journals

citations
Cited by 32 publications
(16 citation statements)
references
References 42 publications
4
12
0
Order By: Relevance
“…6 Previous in vivo studies in independent GFAP-apoE transgenic lines reported no obvious neuropathological alterations in GFAP-E4 mice at baseline, 51,52 consistent with our observations, although in one of these studies 52 GFAP-E4 mice showed a working memory impairment. Another study reported no protective effects of apoE3 or apoE4 in neonatal GFAP-apoE mice after hypoxic-ischemic insults.…”
Section: Discussionsupporting
confidence: 89%
“…6 Previous in vivo studies in independent GFAP-apoE transgenic lines reported no obvious neuropathological alterations in GFAP-E4 mice at baseline, 51,52 consistent with our observations, although in one of these studies 52 GFAP-E4 mice showed a working memory impairment. Another study reported no protective effects of apoE3 or apoE4 in neonatal GFAP-apoE mice after hypoxic-ischemic insults.…”
Section: Discussionsupporting
confidence: 89%
“…43 However, in comparison with strong viral promoters, for example the CMV promoter, it is still weak, as demonstrated in the current study and also several other studies. 14,15 This inherent weakness in driving gene expression might affect the efficacy of certain gene therapy applications in the CNS that require high-level expression of therapeutic genes.…”
Section: Level and Duration Of Transgene Expression In Astrocytessupporting
confidence: 72%
“…Surprisingly, these promoters were not expressed at all in transgenic mice. A possible explanation for the repressed expression of the enhanced gfa2 promoters is that a significant longterm increase in the already robust activity of the gfa2 promoter is toxic (Smith et al, 1998). In support of this hypothesis we have found that when U251 cells are stably transfected with pGfa2(ABD) 3 nLac or the highly active pSVbGal-positive control, the initial high levels of activity are lost with time in culture.…”
Section: Discussionsupporting
confidence: 60%
“…In contrast, about 50% of mice carrying a gfa2-nLac transgene express at substantial levels. A possible explanation for the negative results with the enhanced gfa2 promoters in transgenic mice is that a significant increase in the already robust activity of the gfa2 promoter for an extended time is toxic (Smith et al, 1998). To test this hypothesis, we stably transfected U251 cells with the standard pGfa2nLac plasmid, the enhanced gfa2 plasmid pGfa2(ABD) 3 nLac, or the highly active pSVbGal as a positive control.…”
Section: Expression Of Enhanced Gfa2 Promoters In Transgenic Mice Andmentioning
confidence: 99%