Human Bocavirus genotypes 1 and 2 detected in younger Amazonian children with acute gastroenteritis or respiratory infections, respectively

Leitão, Gabriel Azevedo Alves; Olivares, Alberto Ignácio Olivares; Pimenta, Yan Cardoso; Delgado, Isabella Fernandes; Miagostovich, Marize Pereira; Leite, José Paulo Gagliardi; Moraes, Marcia Terezinha Baroni de

doi:10.1016/j.ijid.2020.03.046

Cited by 13 publications

(19 citation statements)

References 26 publications

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“…Our results corroborate with data from few studies conducted in Brazil that have investigated HBoV in stool samples from AGE cases. Recently, two studies with hospitalized children presenting AGE symptoms from Northern Brazil reported HBoV detection rates of 24% [21] and 12.2% [29]. Albuquerque et al [30] found HBoV in 2% of stool samples (n = 705) from inpatient and outpatient children with AGE symptoms.…”

Section: Discussionmentioning

confidence: 99%

“…In Southeastern Brazil, Santos et al [ 62 ] firstly demonstrated the circulation of HBoV-2 (20.8%) and HBoV-3 (0.6%) in stool samples from patients with AGE. More recently, in Northern Brazil, two studies have reported the circulation of HBoV-1, 2 and 3 in stool samples from children hospitalized for AGE, and both found a predominance of genotype 1 (94.8% and 75%) [ 21 , 29 ]. In line with our findings, studies performed elsewhere have predominantly detected genotypes 1–3 in AGE cases [ 13 , 63 , 64 , 65 ], and HBoV-4 has been rarely detected, or detected in lower prevalence compared to other genotypes [ 12 , 39 , 52 , 60 , 63 , 66 ].…”

Section: Discussionmentioning

confidence: 99%

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Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections

et al. 2020

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Human bocavirus (HBoV) is an emerging virus and has been detected worldwide, especially in pediatric patients with respiratory and gastrointestinal infection. In this study, we describe HBoV prevalence, genotypes circulation and DNA shedding, in stool samples from children up to two years of age in Brazil. During 2016 and 2017, 886 acute gastroenteritis (AGE) stool samples from ten Brazilian states were analyzed by TaqMan®-based qPCR, to detect and quantify HBoV. Positive samples were genotyped by sequencing the VP1/2 overlap region, followed by phylogenetic analysis and co-infections were accessed by screening other gastroenteric viruses. HBoV was detected in 12.4% (n = 110) of samples, with viral load ranging from 1.6 × 102 to 1.2 × 109 genome copies per gram of stool. From these, co-infections were found in 79.1%, and a statistically lower HBoV viral load was found compared to viral loads of rotavirus, norovirus and adenovirus in double infected patients (p < 0.05). No significant differences were found between HBoV viral load in single or co-infections, age groups or genotypes. Phylogenetic analysis identified the circulation of HBoV-1 in 38%, HBoV-2 in 40% and HBoV-3 in 22%. Continuous HBoV monitoring is needed to clarify its role in diarrhea disease, especially in the absence of classic gastroenteric viruses.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Human Bocavirus in Brazil: Molecular Epidemiology, Viral Load and Co-Infections

et al. 2020

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“…For norovirus detection, a duplex (GI/GII) RT-qPCR was performed (Kageyama et al, 2003). For HAdV, a quantitative polymerase chain reaction (qPCR) assay was carried out according to that recently presented (Leitão et al, 2020). All the reactions were conducted on the ABI Prism 7500 Sequence Detection System (Applied Biosystems, California USA) using SuperScript TM III Platinum TM One-Step qRT-PCR (Invitrogen, Carlsbad, California, USA), according to the manufacturer's recommendations, in a total volume of 20 mL and with optimized thermal cycling conditions as described.…”

Section: Virus Detectionmentioning

confidence: 99%

Epidemiology of enteric virus infections in children living in the Amazon region

Olivares

Leitão

Pimenta

et al. 2021

International Journal of Infectious Diseases

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To verify the frequency of viruses causing acute gastroenteritis (AGE) in association with the histo-blood group antigen (HBGA) and Rotarix TM vaccination coverage in children from the Amazon region. Design: Fecal and saliva samples were collected from children with AGE (n = 485) and acute respiratory infection (ARI) (n = 249) clinical symptoms. Rotavirus A (RVA), norovirus, human adenovirus (HAdV), and sapovirus (SaV) were verified in feces by molecular detection. Saliva samples were used for HBGA phenotyping/FUT3 genotyping. Blood group types, clinical aspects and Rotarix TM RVA vaccination data were recorded. Results: Norovirus remained the most prevalently detected cause of AGE (38%, 184/485 and ARI 21.3%, 53/ 249). High HAdV frequencies were observed in AGE children (28.6%, 139/485) and ARI children (37.3%, 93/ 249). RVA was the third most prevalent virus causing AGE (22.7%, 110/485 and ARI 19.3%, 48/249) and a low RV1 coverage (61%, 448/734) was verified. The SaV frequencies were lower (7.2%, 35/485 for AGE and 6.8%, 17/249 for ARI). Secretor children were HBGA susceptible to HAdV infection (OR 1.5, 95% CI 1.0-2.3; P = 0.04) but not to RVA, norovirus or SaV infection. Conclusions: Norovirus could be considered the main etiological agent of AGE. No association was verified for HBGA susceptibility to RVA, norovirus and SaV. Secretor children showed a slight susceptibility to HAdV infection and the Le (a-b-) heterogeneous SNPs on the FUT3 gene.

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“…Previously, no HAstVMLB2 had been detected in Brazil. One hundred and seventy fecal samples, negative for rotavirus A (RVA), GI and GII noroviruses, human adenovirus (HAdV), sapovirus (SaV) (unpublished results) and human bocavirus (HBoV) by quantitative RT-PCR or PCR 5 were selected for this study. These samples were collected from October 2016 to October 2017 from children (≤5 years old) receiving care at “Hospital da Criança de Santo Antonio” (HCSA) in the city of Boa Vista, state of Roraima (RR), Northern Brazil.…”

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confidence: 99%

“…Complementary study design information as well as collecting, processing and total RNA isolation have been reported previously. 5 , 6 , 7 Reverse transcription polymerase chain reaction (RT-PCR) sample screening was carried out initially using an usual and “consensus” protocol with the SF0073/SF0076 (respectively RdRp nucleotide position 3638-3647 and 4042-4024) primers for detection of HAstV1–8, HAstVMLB1−3 and HAstVVA1−3. 8 The segment obtained using these primers has a size of 405 bp from the ORF1b (RNA polymerase) region of the AstV genome.…”

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confidence: 99%