Human bone marrow colony growth in agar‐gel

Pike, Beverley L.; Robinson, William A.

doi:10.1002/jcp.1040760111

Cited by 1,243 publications

(298 citation statements)

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“…Marrow culture technique.-As has been reported previously (Chiyoda et al, 1975), the culture method used was a modificaticn of that described by Pike and Robinson (1970 (Ichikawa, 1969).…”

Section: Methodsmentioning

confidence: 99%

Influence of leukaemic cells on the colony formation of human bone marrow cells in vitro II. Suppressive effects of leukaemic cell extracts

Chiyoda¹,

Mizoguchi²,

Asano³

et al. 1976

Br J Cancer

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The influence of leukaemic cells on the colony formation of human bone marrow cells was studied in vitro as an extension of our previous work (Chiyoda et al., 1975). An extract of leukaemic bone marrow cells significantly suppressed colony forming ability of the normal bone marrow cells, whereas an extract of normal bone marrow cells did not suppress it except in two cases. The suppressive effect of normal bone marrow cells, however, was obviously less intense than that of leukaemic cells. This suppressive effect was dose dependent and was fairly stable to heat treatment. These results suggest that leukaemic bone marrow cells contain factor(s) which suppress normal colony formation.

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“…Marrow culture technique.-As has been reported previously (Chiyoda et al, 1975), the culture method used was a modificaticn of that described by Pike and Robinson (1970 (Ichikawa, 1969).…”

Section: Methodsmentioning

confidence: 99%

Influence of leukaemic cells on the colony formation of human bone marrow cells in vitro II. Suppressive effects of leukaemic cell extracts

Chiyoda¹,

Mizoguchi²,

Asano³

et al. 1976

Br J Cancer

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“…After 14 days of incubation at 37°C in a fully humidified 5% CO 2 atmosphere, colonies of CFU-GM cells were evaluated and scored following standard methods. 19 For CD34 analysis, the cells were incubated for 30 min at 4°C with fluorescein isothiocyanate-conjugated monoclonal antibody (anti-CD34) (Immunotech, Marseille, France). After incubation cell populations were analyzed by flow cytometry as previously described.…”

Section: Pbpc Collection Cryopreservation and Stem Cell Assaysmentioning

confidence: 99%

Factors affecting both peripheral blood progenitor cell mobilization and hematopoietic recovery following autologous blood progenitor cell transplantation in multiple myeloma patients: a monocentric study

et al. 1998

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The aim of the study was to analyze the factors influencing peripheral blood progenitor cell (PBPC) collection after high-dose cyclophosphamide (HDCYC) (7 g/m 2 ) and hematopoietic recovery after autologous transplantation of HDCYC-mobilized PBPC (ABPCT) in 116 patients with aggressive multiple myeloma (MM). Following HDCYC 74 patients received hematopoietic growth factors (HGF), either G-CSF (n = 19) or GM-CSF (n = 55). All the patients were subsequently planned to undergo ABPCT. PBPC collection was possible for 106 patients. The most important prognostic factor for collection of more than 25 × 10 4 CFU-GM cells/kg and 2 × 10 6 CD34 + cells/kg was the use of HGF (P = 0.002 and 0.009, respectively). Previous use of an alkylating agent, response to treatment before HDCYC, and interval between diagnosis and HDCYC were also significant factors (P = 0.004, 0.025 and 0.001, respectively). The number of CFU-GM cells infused was the most important parameter for rapid and complete hematological recovery after ABPCT (P Ͻ 0.0001). Thus the use of HGF post-HDCYC is the major factor which, associated with reduced time between diagnosis and HDCYC and the use of an alkylating agent, could increase the numbers of hematopoietic progenitors collected, and subsequently improve hematopoietic recovery following ABPCT in MM patients.

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“…With the discovery of growth stimulating factors such as CSF (colony stimulating factor) and the introduction of a semisolid agar culture system, actual growth and differentiation of mouse (Pluznik and Sachs, 1965;Bradley and Metcalf, 1966) and human (Pike and Robinson, 1970) bone marrow cells were obtained.…”

mentioning

confidence: 99%

Diagnostic and prognostic significance of peripheral blood cultural characteristics in adult acute leukaemia

Balkwill¹,

Oliver²

1976

Br J Cancer

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Summary.-A simple liquid culture technique has been used to study peripheral blood from patients with acute myelogenous leukaemia. Evidence is presented that cells from morphologically identical types of leukaemia have differing capacity for " differentiation " from free floating blast cells into plastic-adherent, phagocytic, trypsin-resistant macrophage-like cells with Fc and C 3 receptors. Preliminary analysis suggests that patients whose cells have the greatest capacity for " differentiation " have a better chance of achieving complete remission.

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Human bone marrow colony growth in agar‐gel

Cited by 1,243 publications

References 9 publications

Influence of leukaemic cells on the colony formation of human bone marrow cells in vitro II. Suppressive effects of leukaemic cell extracts

Influence of leukaemic cells on the colony formation of human bone marrow cells in vitro II. Suppressive effects of leukaemic cell extracts

Factors affecting both peripheral blood progenitor cell mobilization and hematopoietic recovery following autologous blood progenitor cell transplantation in multiple myeloma patients: a monocentric study

Diagnostic and prognostic significance of peripheral blood cultural characteristics in adult acute leukaemia

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