Human bone marrow stromal cells are efficiently transduced by vesicular stomatitis virus-pseudotyped retrovectors without affecting subsequent osteoblastic differentiation

Liu, P; Kalajzić, Ivo; Stover, Mary Louise; Rowe, D.; Lichtler, Alex

doi:10.1016/s8756-3282(01)00590-7

Cited by 21 publications

(8 citation statements)

References 21 publications

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“…In addition, they are accessible and comparably easy to manipulate, making them natural candidates for orthopedic gene therapy studies [53]. In order to engineer the isolated stem cells several vector system have already been tested on adult mesenchymal stem cells and high transduction and expression rates have been achieved [89,108,119]. However, transforming of isolated mesenchymal stem cells is only one step on the way to its use for cell-based therapy.…”

Section: Stem Cell-based Gene Therapiesmentioning

confidence: 99%

Biological treatment strategies for disc degeneration: potentials and shortcomings

2006

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Recent advances in molecular biology, cell biology and material sciences have opened a new emerging field of techniques for the treatment of musculoskeletal disorders. These new treatment modalities aim for biological repair of the affected tissues by introducing cell-based tissue replacements, genetic modifications of resident cells or a combination thereof. So far, these techniques have been successfully applied to various tissues such as bone and cartilage. However, application of these treatment modalities to cure intervertebral disc degeneration is in its very early stages and mostly limited to experimental studies in vitro or in animal studies. We will discuss the potential and possible shortcomings of current approaches to biologically cure disc degeneration by gene therapy or tissue engineering. Despite the increasing number of studies examining the therapeutic potential of biological treatment strategies, a practicable solution to routinely cure disc degeneration might not be available in the near future. However, knowledge gained from these attempts might be applied in a foreseeable future to cure the low back pain that often accompanies disc degeneration and therefore be beneficial for the patient.

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Section: Stem Cell-based Gene Therapiesmentioning

confidence: 99%

Biological treatment strategies for disc degeneration: potentials and shortcomings

2006

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“…Adult MSCs were also effectively transduced with retroviral vectors pseudotyped with vesicular stomatitis virus (VSV). 65,66 Human bone-marrow-derived adult MSCs were found to be highly susceptible to infection by VSV-pseudotyped retrovirus, achieving transduction rates of greater than 81%. 66 Transduction with VSV did not alter the proliferation and differentiation potential of bone-marrow-derived adult MSCs, confirming the safety of these vectors.…”

Section: Vectors and Promotersmentioning

confidence: 99%

Stem cells as vehicles for orthopedic gene therapy

et al. 2004

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Adult stem cells reside in adult tissues and serve as the source for their specialized cells. In response to specific factors and signals, adult stem cells can differentiate and give rise to functional tissue specialized cells. Adult mesenchymal stem cells (MSCs) have the potential to differentiate into various mesenchymal lineages such as muscle, bone, cartilage, fat, tendon and ligaments. Adult MSCs can be relatively easily isolated from different tissues such as bone marrow, fat and muscle. Adult MSCs are also easy to manipulate and expand in vitro. It is these properties of adult MSCs that have made them the focus of cellmediated gene therapy for skeletal tissue regeneration. Adult MSCs engineered to express various factors not only deliver them in vivo, but also respond to these factors and differentiate into skeletal specialized cells. This allows them to actively participate in the tissue regeneration process. In this review, we examine the recent achievements and developments in stem-cell-based gene therapy approaches and their applications to bone, cartilage, tendon and ligament tissues that are the current focus of orthopedic medicine.

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“…Recently, the use of a Lentiviral vector encoding for the BMP-2 gene has been shown to be effective in expressing the transgene in rat MSCs leading to osteogenesis in vitro and in vivo [ 82,83 ] . MSCs were also effectively transduced with other vectors such as vesicular stomatitis virus (VSV) [84][85][86] . Human bone marrow-derived MSCs were found to be highly susceptible to VSV infection achieving high rates of transduction with more than 81% effi ciency [ 84 ] .…”

Section: Genetic Engineering Of Mscsmentioning

confidence: 99%

“…MSCs were also effectively transduced with other vectors such as vesicular stomatitis virus (VSV) [84][85][86] . Human bone marrow-derived MSCs were found to be highly susceptible to VSV infection achieving high rates of transduction with more than 81% effi ciency [ 84 ] . A safety aspect was also encountered as transduction with VSV did not alter the proliferation and differentiation potential of bone marrow-derived MSCs.…”

Section: Genetic Engineering Of Mscsmentioning

confidence: 99%