Human cancer cell lines: Experimental models for cancer cells in situ? For cancer stem cells?

Staveren, Wilma C.G. van; Solis, David; Hebrant, Aline; Detours, Vincent; Dumont, Jacques Emile; Maenhaut, Carine

doi:10.1016/j.bbcan.2008.12.004

Cited by 164 publications

(212 citation statements)

References 114 publications

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“…Whether cancer cell lines are reliable representatives of in vivo cancer biology is, however, still a matter of debate. Cancer cell lines are developed under selection pressure and, after many passages in vitro, may no longer be representative of the original primary culture from which the cell line was derived (21,24). In line with this, changes in gene expression were observed during the establishment of SCLC cell lines (25).…”

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confidence: 80%

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Array comparative genomic hybridization-based characterization of genetic alterations in pulmonary neuroendocrine tumors

Voortman

Lee

Killian

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

120

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The goal of this study was to characterize and classify pulmonary neuroendocrine tumors based on array comparative genomic hybridization (aCGH). Using aCGH, we performed karyotype analysis of 33 small cell lung cancer (SCLC) tumors, 13 SCLC cell lines, 19 bronchial carcinoids, and 9 gastrointestinal carcinoids. In contrast to the relatively conserved karyotypes of carcinoid tumors, the karyotypes of SCLC tumors and cell lines were highly aberrant. High copy number (CN) gains were detected in SCLC tumors and cell lines in cytogenetic bands encoding JAK2, FGFR1, and MYC family members. In some of those samples, the CN of these genes exceeded 100, suggesting that they could represent driver alterations and potential drug targets in subgroups of SCLC patients. In SCLC tumors, as well as bronchial carcinoids and carcinoids of gastrointestinal origin, recurrent CN alterations were observed in 203 genes, including the RB1 gene and 59 microRNAs of which 51 locate in the DLK1-DIO3 domain. These findings suggest the existence of partially shared CN alterations in these tumor types. In contrast, CN alterations of the TP53 gene and the MYC family members were predominantly observed in SCLC. Furthermore, we demonstrated that the aCGH profile of SCLC cell lines highly resembles that of clinical SCLC specimens. Finally, by analyzing potential drug targets, we provide a genomics-based rationale for targeting the AKT-mTOR and apoptosis pathways in SCLC.carcinoid | cell line | gene dosage | small cell lung carcinoma | therapy

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confidence: 80%

“…Many lung-cancer cell lines have been developed and extensively used as research tools to study cancer biology and to aid in drug development (21)(22)(23). Whether cancer cell lines are reliable representatives of in vivo cancer biology is, however, still a matter of debate.…”

mentioning

confidence: 99%

Array comparative genomic hybridization-based characterization of genetic alterations in pulmonary neuroendocrine tumors

Voortman

Lee

Killian

et al. 2010

Proc. Natl. Acad. Sci. U.S.A.

120

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“…Although tumor cell line models, as the NCI60 and other panels, can provide important information on mechanisms of action of anticancer drugs, the correlation between the activity in cell lines and in patients with the corresponding tumor types is not well documented and screening for cancer activity using PCPTC could potentially be an alternative [3,28]. In vitro response analysis of PCPTCs reports accurate diagnosis specific activity of cancer drugs [7] and predict clinical response for individual patients [6].…”

Section: Discussionmentioning

confidence: 99%

Phenotype-based drug screening in primary ovarian carcinoma cultures identifies intracellular iron depletion as a promising strategy for cancer treatment

Gullbo

Fryknäs

Rickardson

et al. 2011

Biochemical Pharmacology

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This is a PDF file of an unedited manuscript that has been accepted for publication. As a service to our customers we are providing this early version of the manuscript. The manuscript will undergo copyediting, typesetting, and review of the resulting proof before it is published in its final form. Please note that during the production process errors may be discovered which could affect the content, and all legal disclaimers that apply to the journal pertain. The results indicate the feasibility of using PCPTC for cancer drug screening and that intracellular iron depletion could be a potentially important strategy for cancer therapy.

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“…Furthermore, when infected with C. albicans, hBD2 was markedly up-regulated in the FTOM and EpiOral™ models in a similar manner to that described for the normal oral mucosa [28,29], whereas the RHOE model showed only very minor increases in expression. The disparities in the expression of these immune molecules between models are likely to be due to genetic differences between normal and tumor cells where gene expression of cytokines, pattern recognition receptors, anti-microbial peptides and cell signaling molecules are often dysregulated [37,38].…”

Section: Discussionmentioning

confidence: 99%

Evaluation of tissue engineered models of the oral mucosa to investigate oral candidiasis

Yadev

Murdoch

Saville

et al. 2011

Microbial Pathogenesis

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Candida albicans is a commensal organism that can be isolated from the majority of healthy individuals. However, in certain susceptible individuals C.albicans can become pathogenic leading to the mucocutaneous infection; oral candidiasis. Murine models and in vitro monolayer cultures have generated some data on the likely virulence and host factors that contribute to oral candidiasis but these models have limitations. Recently, tissue engineered oral mucosal models have been developed to mimic the normal oral mucosa but little information is available on their true representation. In this study we assessed the histological features of three different tissue engineered oral mucosal models compared to the normal oral mucosa and analysed both cell damage and cytokine release following infection with C.albicans. Models comprised of normal oral keratinocytes and a fibroblast-containing matrix displayed more similar immunohistological and proliferation characteristics to normal mucosa compared to models composed of an oral carcinoma cell line. Although all models were invaded and damaged by C.albicabs in a similar manner, the cytokine response was much more pronounced in models containing normal keratinocytes. These data suggest that models based on normal keratinocytes atop a fibroblast-containing connective tissue will significantly aid in dissecting the molecular pathogenesis of oral candidiasis.

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Human cancer cell lines: Experimental models for cancer cells in situ? For cancer stem cells?

Cited by 164 publications

References 114 publications

Array comparative genomic hybridization-based characterization of genetic alterations in pulmonary neuroendocrine tumors

Array comparative genomic hybridization-based characterization of genetic alterations in pulmonary neuroendocrine tumors

Phenotype-based drug screening in primary ovarian carcinoma cultures identifies intracellular iron depletion as a promising strategy for cancer treatment

Evaluation of tissue engineered models of the oral mucosa to investigate oral candidiasis

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