“…The basal cell layers appear flattened. This agrees with other corneal construct studies done before [25–28] and also in corneal in-vivo .10.1080/19932820.2018.1500347-F0001Figure 1.Human Corneal cell construct.…”
Section: Resultssupporting
confidence: 89%
“…The cells were thawed and seeded and cultured in Medium F99, which is the amalgamation of Ham’s F12 and Medium F99 in the ratio of 1:1 and 5% FCS and 1% antibiotic/antimycotic solution was added to above media according to the instructions provided [25–28]. …”
Section: Methodsmentioning
confidence: 99%
“…A step by step in-vitro corneal cell construct was prepared from the HENC as described by Reichl et al and Kawazu et al [25,29]. 2 × 10 6 cells were seeded onto a 4.7 cm 2 surface area containing polycarbonate filter with pore size of 3.0 mm.…”
Section: Methodsmentioning
confidence: 99%
“…The drug permeation studies were done over the human corneal construct. The protocol was adapted from Reichl et al [25] with some necessary modifications. The donor drug formulation had NAC 2% and SLN NAC 2%.…”
Section: Methodsmentioning
confidence: 99%
“…Calculation of permeation coefficient K P was conceded as drug concentration from the rectilinear gradient of a permeation curve. Apart from this, drug permeation was also seen by staining the corneal constructs with H&E as per the protocol described by Reichl et al [25]. …”
The purpose of this study is to prepare and characterize solid lipid nanoparticles (SLN) of N-Acetyl Carnosine (NAC) to treat cataract since surgery necessitates equipments and professional help. Cataract is believed to be formed by the biochemical approach where the crystalline eye proteins lose solubility and forms high molecular weight masses. Added advantages of SLN of NAC (henceforth referred as SLN-NAC) in the study are reduced size, sustained release and better corneal penetration of drug. The method of preparation of SLN-NAC by Mill’s method is unique in itself.The size of the SLN-NAC was 75 ± 10 nm in the range of ideal for penetration. The in-vitro release study and the SLN-NAC formulations prepared with Mill’s method demonstrated sustained release up to 24 h following an initial burst after 1 h. The zeta potential of the prepared formulation was −22.1 ± 1 mV. Corneal permeation studies using goat corneas indicate that SLN-NAC penetration rate was higher than those from NAC eye drops. Corneal hydration studies indicated that the formulation caused no harm to the corneal cells.Therefore it may be concluded that SLN-NAC may revolutionize cataract treatment and reversal by improving drug permeation, reducing toxicity and no damage to corneal tissue.
“…The basal cell layers appear flattened. This agrees with other corneal construct studies done before [25–28] and also in corneal in-vivo .10.1080/19932820.2018.1500347-F0001Figure 1.Human Corneal cell construct.…”
Section: Resultssupporting
confidence: 89%
“…The cells were thawed and seeded and cultured in Medium F99, which is the amalgamation of Ham’s F12 and Medium F99 in the ratio of 1:1 and 5% FCS and 1% antibiotic/antimycotic solution was added to above media according to the instructions provided [25–28]. …”
Section: Methodsmentioning
confidence: 99%
“…A step by step in-vitro corneal cell construct was prepared from the HENC as described by Reichl et al and Kawazu et al [25,29]. 2 × 10 6 cells were seeded onto a 4.7 cm 2 surface area containing polycarbonate filter with pore size of 3.0 mm.…”
Section: Methodsmentioning
confidence: 99%
“…The drug permeation studies were done over the human corneal construct. The protocol was adapted from Reichl et al [25] with some necessary modifications. The donor drug formulation had NAC 2% and SLN NAC 2%.…”
Section: Methodsmentioning
confidence: 99%
“…Calculation of permeation coefficient K P was conceded as drug concentration from the rectilinear gradient of a permeation curve. Apart from this, drug permeation was also seen by staining the corneal constructs with H&E as per the protocol described by Reichl et al [25]. …”
The purpose of this study is to prepare and characterize solid lipid nanoparticles (SLN) of N-Acetyl Carnosine (NAC) to treat cataract since surgery necessitates equipments and professional help. Cataract is believed to be formed by the biochemical approach where the crystalline eye proteins lose solubility and forms high molecular weight masses. Added advantages of SLN of NAC (henceforth referred as SLN-NAC) in the study are reduced size, sustained release and better corneal penetration of drug. The method of preparation of SLN-NAC by Mill’s method is unique in itself.The size of the SLN-NAC was 75 ± 10 nm in the range of ideal for penetration. The in-vitro release study and the SLN-NAC formulations prepared with Mill’s method demonstrated sustained release up to 24 h following an initial burst after 1 h. The zeta potential of the prepared formulation was −22.1 ± 1 mV. Corneal permeation studies using goat corneas indicate that SLN-NAC penetration rate was higher than those from NAC eye drops. Corneal hydration studies indicated that the formulation caused no harm to the corneal cells.Therefore it may be concluded that SLN-NAC may revolutionize cataract treatment and reversal by improving drug permeation, reducing toxicity and no damage to corneal tissue.
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