Human Cytomegalovirus Long Non-coding RNA1.2 Suppresses Extracellular Release of the Pro-inflammatory Cytokine IL-6 by Blocking NF-κB Activation

Lau, Betty; Kerr, Karen; Gu, Quan; Nightingale, Katie; Antrobus, Robin; Suárez, Nicolás M.; Stanton, Richard J.; Wang, Edward Chung Yern; Weekes, Michael P.; Davison, Andrew J.

doi:10.3389/fcimb.2020.00361

Cited by 13 publications

(13 citation statements)

References 67 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Although annotated as a long RNA lacking a canonical ORF, RNA1.2 ribosome occupancy has been demonstrated and unique peptides encoded by RNA1.2 detected ( Stern-Ginossar et al, 2012 ). Reduced RNA1.2 expression reportedly results in NF-kB activation and could impact virus replication by regulating host genes ( Lau et al, 2020 ). Finally, eight transcripts encoded by TB40/E, but not present in the AD169 laboratory strain, were regulated by eIF3d.…”

Section: Resultsmentioning

confidence: 99%

An eIF3d-dependent switch regulates HCMV replication by remodeling the infected cell translation landscape to mimic chronic ER stress

et al. 2022

View full text Add to dashboard Cite

Section: Resultsmentioning

confidence: 99%

An eIF3d-dependent switch regulates HCMV replication by remodeling the infected cell translation landscape to mimic chronic ER stress

et al. 2022

View full text Add to dashboard Cite

“…However, miRNAs have specific motifs, none of which are predicted or have been detected experimentally within proximity of the m15 locus [ 31 , 42 , 43 , 44 ]. Recently, functional long non-coding RNAs (lncRNAs) have been described in all herpesvirus families [ 45 , 46 , 47 ]. Four major lncRNAs, representing more than half of the polyadenylated transcripts detected, have been described in HCMV, none of which overlap protein-coding genes [ 48 , 49 ].…”

Section: Discussionmentioning

confidence: 99%

The m15 Locus of Murine Cytomegalovirus Modulates Natural Killer Cell Responses to Promote Dissemination to the Salivary Glands and Viral Shedding

et al. 2021

View full text Add to dashboard Cite

As the largest herpesviruses, the 230 kb genomes of cytomegaloviruses (CMVs) have increased our understanding of host immunity and viral escape mechanisms, although many of the annotated genes remain as yet uncharacterised. Here we identify the m15 locus of murine CMV (MCMV) as a viral modulator of natural killer (NK) cell immunity. We show that, rather than discrete transcripts from the m14, m15 and m16 genes as annotated, there are five 3′-coterminal transcripts expressed over this region, all utilising a consensus polyA tail at the end of the m16 gene. Functional inactivation of any one of these genes had no measurable impact on viral replication. However, disruption of all five transcripts led to significantly attenuated dissemination to, and replication in, the salivary glands of multiple strains of mice, but normal growth during acute infection. Disruption of the m15 locus was associated with heightened NK cell responses, including enhanced proliferation and IFNγ production. Depletion of NK cells, but not T cells, rescued salivary gland replication and viral shedding. These data demonstrate the identification of multiple transcripts expressed by a single locus which modulate, perhaps in a concerted fashion, the function of anti-viral NK cells.

show abstract

“…Depletion of this lncRNA decreases CMVV DNA replication and its growth. Notably, CRISPR-Cas9-mediated targeting the RNA4.9 promoter results in the reduction of viral ssDBP levels implying the relation between ssDBP levels and oriLyt activity [ 30 ].…”

Section: Ncrnas and CMV Infectionmentioning

confidence: 99%

The Emerging Role of Non-Coding RNAs in the Regulation of Virus Replication and Resultant Cellular Pathologies

Ghafouri‐Fard

Hussen

Jamal

et al. 2022

IJMS

View full text Add to dashboard Cite

Non-coding RNAs, particularly lncRNAs and miRNAs, have recently been shown to regulate different steps in viral infections and induction of immune responses against viruses. Expressions of several host and viral lncRNAs have been found to be altered during viral infection. These lncRNAs can exert antiviral function via inhibition of viral infection or stimulation of antiviral immune response. Some other lncRNAs can promote viral replication or suppress antiviral responses. The current review summarizes the interaction between ncRNAs and herpes simplex virus, cytomegalovirus, and Epstein–Barr infections. The data presented in this review helps identify viral-related regulators and proposes novel strategies for the prevention and treatment of viral infection.

show abstract

Human Cytomegalovirus Long Non-coding RNA1.2 Suppresses Extracellular Release of the Pro-inflammatory Cytokine IL-6 by Blocking NF-κB Activation

Abstract: and Davison AJ (2020) Human Cytomegalovirus Long Non-coding RNA1.2 Suppresses Extracellular Release of the Pro-inflammatory Cytokine IL-6 by Blocking NF-κB Activation.

Cited by 13 publications

References 67 publications

An eIF3d-dependent switch regulates HCMV replication by remodeling the infected cell translation landscape to mimic chronic ER stress

An eIF3d-dependent switch regulates HCMV replication by remodeling the infected cell translation landscape to mimic chronic ER stress

The m15 Locus of Murine Cytomegalovirus Modulates Natural Killer Cell Responses to Promote Dissemination to the Salivary Glands and Viral Shedding

The Emerging Role of Non-Coding RNAs in the Regulation of Virus Replication and Resultant Cellular Pathologies

Contact Info

Product

Resources

About