Human delta-aminolevulinate dehydratase: nucleotide sequence of a full-length cDNA clone.

Wetmur, James G.; Bishop, David F.; Cantelmo, Christopher; Desnick, Robert J.

doi:10.1073/pnas.83.20.7703

Cited by 117 publications

(93 citation statements)

References 41 publications

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“…ALA has neurotoxic activity and may contribute to lead-induced toxicity to the brain (36 Petrucci and colleagues (38). Later, the cDNA was cloned and the gene sequenced by Wetmur and coworkers (39,40). The ALAD2 allele is the least common form, occurring in 20% of the Caucasian population and more rarely in populations of African and Asian descent (24,38,41).…”

Section: -Aminolevulinic Acid Dehydratase Genementioning

confidence: 99%

Genetic Susceptibility to Lead Poisoning

Onalaja¹,

Cláudio²

2000

Environmental Health Perspectives

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Section: -Aminolevulinic Acid Dehydratase Genementioning

confidence: 99%

Genetic Susceptibility to Lead Poisoning

Onalaja¹,

Cláudio²

2000

Environmental Health Perspectives

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“…Positions with identical amino acid residues in all five polypeptides are marked by asterisks. The upper box indicates the tentative zinc binding region (57), and the lower box indicates the sequence containing the lysine residue in the active site (18). The sequences are from references 11, 31, 40, 57 and this work.…”

Section: 1mentioning

confidence: 99%

“…5). Human and bovine liver PBG synthase are homooctamers, with subunits of 35 kDa (54,57). In Spinacia oleracea, the enzyme is a homohexamer of 50-kDa subunits (33).…”

Section: 1mentioning

confidence: 99%

“…6), and Gibbs and Jordan (18) have proposed that this zinc region plays a structural rather than a catalytical role. Wetmur et al (57) found that the zinc binding region of human PBG synthase shows similarities to the consensus sequence of the zinc-chelating domain in zinc fingers (10,30) and suggested Cys-119, Cys-122, His-129, and Cys-132 as zinc ligands, but they also considered Cys-124 and His-131 possible ligands. The Cys corresponding to Cys-119 in human PBG synthase is not conserved in E. coli and B. subtilis.…”

Section: 1mentioning

confidence: 99%

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The Bacillus subtilis hemAXCDBL gene cluster, which encodes enzymes of the biosynthetic pathway from glutamate to uroporphyrinogen III

et al. 1991

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We have recently reported (M. Petricek, L. Rutberg, I. Schröder, and L. Hederstedt, J. Bacteriol. 172: 2250-2258, 1990) the cloning and sequence of a Bacillus subtilis chromosomal DNA fragment containing hemA proposed to encode the NAD(P)H-dependent glutamyl-tRNA reductase of the C5 pathway for 5-aminolevulinic acid (ALA) synthesis, hemX encoding a hydrophobic protein of unknown function, and hemC encoding hydroxymethylbilane synthase. In the present communication, we report the sequences and identities of three additional hem genes located immediately downstreatm of hemC, namely, hemD encoding uroporphyrinogen III synthase, hemB encoding porphobilinogen synthase, and hemL encoding glutamate-1-semialdehyde 2,1-aminotransferase. The six genes are proposed to constitute a hem operon encoding enzymes required for the synthesis of uroporphyrinogen III from glutamyl-tRNA. hemA, hemB, hemC, and hemD have all been shown to be essential for heme synthesis. However, deletion of an internal 427-bp fragment of hemL did not create a growth requirement for ALA or heme, indicating that formation of ALA from glutamate-1-semialdehyde can occur spontaneously in vivo or that this reaction may also be catalyzed by other enzymes. An analysis of B. subtilis carrying integrated plasmids or deletions-substitutions in or downstream of hemL indicates that no further genes in heme synthesis are part of the proposed hem operon.

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“…Na via de síntese do Heme, a atividade catalítica da ALAD é a condensação assimétrica de duas moléculas de ácido delta aminolevulínico com a formação de uma molécula de porfibilinogênio, primeiro precursor do Heme (WETMUR et al, 1986) A atividade da ALAD, em eritrócitos humanos, tem sido utilizada como uma indicação altamente específica e sensível às exposições ao chumbo, uma vez que a enzima tem alta afinidade in vivo às baixas concentrações de chumbo no sangue. Essa inibição ocorre devido à substituição de Zn+2 por Pb+2 na ligação aos radicais SH do sítio de ligação ao cofator metálico (HAEGER-ARONSEN et al, 1971;GOERING, 1993).…”

Section: áCido Delta-aminolevulínico Desidratase (Alad)unclassified

Biomarkers as tools in quality assessment of fish contaminated with trace metals

Coimbra¹,

Santos²,

Saraiva³

et al. 2013

Boletim Do Observatório Ambiental Alberto Ribeiro Lamego

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ResumoMetais traço que apresentem toxicidade aos vertebrados são alvo de preocupação por parte dos programas de monitoramento ambiental em função do impacto causado por ação da atividade antrópica. A distribuição destes elementos se diferencia nas matrizes: água, sedimento e biota. Metais como Pb, Cd, Hg, As e Cr são relacionados a importantes danos ambientais, podendo contaminar o homem principalmente por consumo de pescado. Alguns biomarcadores podem ser aplicados na abordagem de impacto e avaliação de riscos para populações de espécies suscetíveis à exposição e efeito dos metais traço tóxicos. A acetilcolinesterase, a metalotioneína, o ácido delta aminolevulínico desidratase e o ensaio de micronúcleo podem ser utilizados em conjunto como biomarcadores quando organismos aquáticos são expostos ou afetados por metais tóxicos. A resolução 357/2005 do CONAMA no Ministério do Meio Ambiente e a Portaria 2914/2011 do Ministério da Saúde estabelecem limites máximos desses metais tóxicos em água superficial e água de consumo humano respectivamente. Entretanto, ainda não se exige a determinação das espécies químicas, relevante à biodisponibilidade produzida nas diferentes matrizes, nem sobre os efeitos subletais, avaliados pelos biomarcadores, destes metais nos organismos aquáticos. A proposta desta revisão é apresentar os biomarcadores (bioquímicos e genéticos) como ferramentas auxiliares na avaliação da qualidade de pescado.Palavras-chave: Metais traço. Biomarcadores. Peixe.

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Human delta-aminolevulinate dehydratase: nucleotide sequence of a full-length cDNA clone.

Cited by 117 publications

References 41 publications

Genetic Susceptibility to Lead Poisoning

Genetic Susceptibility to Lead Poisoning

The Bacillus subtilis hemAXCDBL gene cluster, which encodes enzymes of the biosynthetic pathway from glutamate to uroporphyrinogen III

Biomarkers as tools in quality assessment of fish contaminated with trace metals

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