Human-Derived Bifidobacterium dentium Modulates the Mammalian Serotonergic System and Gut–Brain Axis

Engevik, Melinda A.; Luck, Berkley; Visuthranukul, Chonnikant; Ihekweazu, Faith D.; Engevik, Amy C.; Shi, Zhongcheng; Danhof, Heather A.; Chang‐Graham, Alexandra L.; Hall, Anne; Endres, Bradley T.; Haidacher, Sigmund J.; Horvath, Thomas D.; Haag, Anthony M.; Devaraj, Sridevi; Garey, Kevin W.; Britton, Robert A.; Hyser, Joseph M.; Shroyer, Noah F.; Versalovic, James

doi:10.1016/j.jcmgh.2020.08.002

Cited by 93 publications

(83 citation statements)

References 151 publications

(226 reference statements)

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“…The gut–brain axis describes the bidirectional connection between the cognitive and emotional centers of the brain with that of the peripheral intestine through the vagus nerve of the central nervous system [ 68 ]. The Bifidobacterium , Lactobacillus plantarum , and Akkermansia muciniphila associated with gallotannin metabolism within the gut has been described by others to increase serotonin signaling [ 68 , 69 , 70 ]. Bacteroides thetaiotaomicron is attributed to the motility of the gastrointestinal tract [ 71 ].…”

Section: Interactions Between Mango Polyphenols and The Intestinal Microbiotamentioning

confidence: 99%

Mango (Mangifera indica L.) Polyphenols: Anti-Inflammatory Intestinal Microbial Health Benefits, and Associated Mechanisms of Actions

et al. 2021

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Mango is rich in polyphenols including gallotannins and gallic acid, among others. The bioavailability of mango polyphenols, especially polymeric gallotannins, is largely dependent on the intestinal microbiota, where the generation of absorbable metabolites depends on microbial enzymes. Mango polyphenols can favorably modulate bacteria associated with the production of bioactive gallotannin metabolites including Lactobacillus plantarum, resulting in intestinal health benefits. In several studies, the prebiotic effects of mango polyphenols and dietary fiber, their potential contribution to lower intestinal inflammation and promotion of intestinal integrity have been demonstrated. Additionally, polyphenols occurring in mango have some potential to interact with intestinal and less likely with hepatic enzymes or transporter systems. This review provides an overview of interactions of mango polyphenols with the intestinal microbiome, associated health benefits and underlying mechanisms.

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Section: Interactions Between Mango Polyphenols and The Intestinal Microbiotamentioning

confidence: 99%

Mango (Mangifera indica L.) Polyphenols: Anti-Inflammatory Intestinal Microbial Health Benefits, and Associated Mechanisms of Actions

et al. 2021

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“…Within the family Bifidobacteriaceae three species, Bifidobacterium dentium, Parascardovia denticolens, and Scardovia inopinata, are present as an abundant population in the oral cavity [49]. In a recent study [50], B. dentium was reported to successfully colonize the germ-free mice ileum and colon. However, the evaluation of the influence of prebiotics on its adherence was not the objective of the study.…”

Section: Discussionmentioning

confidence: 99%

Identification of Synbiotics Conducive to Probiotics Adherence to Intestinal Mucosa Using an In Vitro Caco-2 and HT29-MTX Cell Model

et al. 2021

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The ability of bacteria to adhere to the intestinal mucosa is a critical property necessary for the long-term colonization of the intestinal tract. This ability can be highly sensitive to the presence of prebiotics. However, limited data are available in this respect for beneficial bacteria such as probiotics or resident gut microbiota. We previously demonstrated that the presence of prebiotics may decrease adherence in several pre- and probiotic combinations. Thus, characterizing the interactions between numerous combinations involving different classes of pre- and probiotics can be crucial in identifying new synbiotics. Accordingly, here, we extend our prior analyses to evaluate the adhesion of five lactobacilli, six bifidobacteria, and one probiotic Escherichia coli strains, as commercial probiotics or promising probiotic candidates, together with the cariogenic Bifidobacterium dentium strain. As an in vitro intestinal mucosa model, Caco-2 and mucin-secreting HT29-MTX cells were co-cultured at 9:1 in the presence or absence of prebiotics. Commercial inulin-type fructooligosaccharide prebiotics Orafti® GR, Orafti® P95, and galactooligosaccharide-based prebiotic formula Vivinal®, including purified human milk oligosaccharides (HMOs) were added into the cultivation media as the sole sugar source (2.5% each). Adherence was tested using microtiter plates and was evaluated as the percentage of fluorescently labeled bacteria present in the wells after three washes. Consistent prebiotics-mediated enhanced adherence was observed only for the commercial probiotic strain E. coli O83. For the remaining strains, the presence of HMO or prebiotics Orafti® P95 or Orafti® GR decreased adherence, reaching statistical significance (p < 0.05) for three of out of eight (HMO) or five of out of 11 strains tested, respectively. Conversely, Vivinal® enhanced adhesion in six out of the 12 strains tested, and notably, it significantly attenuated the adherence of the cariogenic Bifidobacterium dentium Culture Collection of Dairy Microorganisms (CCDM) 318. To our knowledge, this represents the first report on the influence of commercial prebiotics and HMOs on the adhesion of the cariogenic Bifidobacterium sp. Vivinal® seems to be a promising prebiotic to be used in the formulation of synbiotics, supporting the adhesion of a wide range of probiotics, especially the strains B. bifidum BBV and BBM and the probiotic Escherichia coli O83.

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“…Tryptic digest samples were chromatographically separated on a Dionex Ultimate 3000 RSLC nano-system (Thermo Scientific) using an Acclaim PepmapTM C-18 capillary column (75 μm (ID) × 150 mm (L), Thermo Scientific) outfitted with an Acclaim PepmapTM C18 trap column (100 μm (ID) × 20 mm (L), Thermo Scientific). Chromatography was performed as previously described [ 22 ]. Elution gradients were prepared from an aqueous mobile phase (A) of H2O:ACN:FA (94.9:5:0.1 v/v/v ) and an organic mobile phase (B) of ACN:FA (99.9:0.1 v/v).…”

Section: Methodsmentioning

confidence: 99%

The metabolic profile of Bifidobacterium dentium reflects its status as a human gut commensal

et al. 2021

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Background Bifidobacteria are commensal microbes of the mammalian gastrointestinal tract. In this study, we aimed to identify the intestinal colonization mechanisms and key metabolic pathways implemented by Bifidobacterium dentium. Results B. dentium displayed acid resistance, with high viability over a pH range from 4 to 7; findings that correlated to the expression of Na+/H+ antiporters within the B. dentium genome. B. dentium was found to adhere to human MUC2+ mucus and harbor mucin-binding proteins. Using microbial phenotyping microarrays and fully-defined media, we demonstrated that in the absence of glucose, B. dentium could metabolize a variety of nutrient sources. Many of these nutrient sources were plant-based, suggesting that B. dentium can consume dietary substances. In contrast to other bifidobacteria, B. dentium was largely unable to grow on compounds found in human mucus; a finding that was supported by its glycosyl hydrolase (GH) profile. Of the proteins identified in B. dentium by proteomic analysis, a large cohort of proteins were associated with diverse metabolic pathways, indicating metabolic plasticity which supports colonization of the dynamic gastrointestinal environment. Conclusions Taken together, we conclude that B. dentium is well adapted for commensalism in the gastrointestinal tract.

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Human-Derived Bifidobacterium dentium Modulates the Mammalian Serotonergic System and Gut–Brain Axis

Cited by 93 publications

References 151 publications

Mango (Mangifera indica L.) Polyphenols: Anti-Inflammatory Intestinal Microbial Health Benefits, and Associated Mechanisms of Actions

Mango (Mangifera indica L.) Polyphenols: Anti-Inflammatory Intestinal Microbial Health Benefits, and Associated Mechanisms of Actions

Identification of Synbiotics Conducive to Probiotics Adherence to Intestinal Mucosa Using an In Vitro Caco-2 and HT29-MTX Cell Model

The metabolic profile of Bifidobacterium dentium reflects its status as a human gut commensal

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