Human embryonic kidney (HEK-293) cell line: An alternative for rabies virus diagnosis and research

Zamudio, Raphaela Mello; Garcia, Jaíne Gonçalves; Barboza, Camila Mosca; Rodrigues, A. C.; Arruda, Tamires Santos de; Francisco, Ana Lee Aparecida; Fahl, Willian de Oliveira; Castilho, Juliana Galera; Silva, Maria Cristina Carlan da; Batista, Helena Beatriz de Carvalho Ruthner

doi:10.1016/j.jviromet.2021.114195

Cited by 1 publication

(1 citation statement)

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“…Murine neuroblastoma (N2A) cells used in this study were kindly provided by the Australian Animal Health Laboratory (AAHL, Gelong, Australia). This experiment was performed according to a previously published method [ 17 ] with minor modifications. The cells were first grown in a tissue culture flask (Iwaki, Japan) using complete DMEM (CDMEM, Thermo Fisher Scientific, Massachusetts, USA) containing 0.1 mL of heat-inactivated fetal calf serum, 2 g/mL of gentamicin, and 0.3 g/mL of amphotericin B to obtain approximately 6.0 × 10 5 cells/mL.…”

Section: Methodsmentioning

confidence: 99%

Epidemiological and viral studies of rabies in Bali, Indonesia

Tenaya,

Suartha,

Suarsana

et al. 2023

Vet World

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Background and Aim: Rabies has been endemic in Bali since 2009, and cases has recently increased. Unfortunately, there is a lack of available vaccines, which hinders the eradication program. This study aimed to investigate the epidemiological and virological aspects of rabies infection in Bali. Materials and Methods: A total of 24 brain samples were collected from rabid dogs in all districts of Bali. The samples were tested using the direct fluorescent antibody (DFA) test and polymerase chain reaction (PCR) to confirm the presence of rabies virus in the samples. Samples with the highest virus content were propagated in vivo and then inoculated into BALB/c mice. The brains of dead mice were used to prepare an inoculate cultured in murine neuroblastoma cells. Supernatant-positive viruses representing each district were then reinoculated into eight groups of five BALB/c mice. A brain sample from each dead mouse was tested using DFA and PCR and detected under a fluorescence microscope. Results: All rabies virus-positive samples collected from rabid dogs in all districts of Bali were positive. Rabies virus was detected by DFA test and PCR and was consistently confirmed in the in vivo and in vitro studies. BALB/c mice inoculated with the highest viral dilution (105 cells/mL) of culture supernatant showed typical signs of rabies, indicating that the virus could be properly investigated. Conclusion: This study demonstrated a wide epidemiological distribution of rabies in Bali. The obtained virus can be adapted for in vitro and in vivo studies and can be used to develop a homologous vaccine. Keywords: Bali, dogs, polymerase chain reaction, rabies virus.

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Section: Methodsmentioning

confidence: 99%