Human Endometrial Stem Cells May  Differentiate into Schwann Cells in  Fibrin Gel as 3D Culture

Bayat, Noushin; Ebrahimi‐Barough, Somayeh; Ardakan, Mohammad Mehdi Mokhtari; Ai, Jafar

doi:10.4236/nm.2015.64024

Cited by 3 publications

(2 citation statements)

References 32 publications

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“…Synthetic materials have several limitations like biocompatibility concerns, the inability to support cell attachment, and undesirable degradation rate [12,[15][16][17]. In contrast, fibrin gels as biopolymeric materials provides numerous advantages over synthetic materials such as excellent biocompatibility, nontoxic degradation products, promotion of cell attachment, controllable degradation rate which matches those of retinal tissue regeneration, and degradation in a controllable manner [18][19][20]. Moreover, the morphology, mechanical properties, and stability of fibrin hydrogel could be tuned by controlling the precursor concentration and ionic strength [21,22].…”

Section: Introductionmentioning

confidence: 99%

Fibrin gel as a scaffold for photoreceptor cells differentiation from conjunctiva mesenchymal stem cells in retina tissue engineering

Soleimannejad

Ebrahimi‐Barough

Soleimani

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

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Stem cell-based therapies are attraction approaches for regenerative medicine for treating retinal diseases. One of the limitations in cell therapy is cell death following post-injection whit preventing functional integration with retinal tissue. Fibrin gel, a bio-polymeric material with excellent biocompatibility, provides numerous advantages as a tissue engineering scaffold and a stem cell carrier. Therefore, current research is focusing on developing fibrin hydrogel scaffolds to protect stem cells during delivery and to stimulate endogenous regeneration through interactions of transplanted stem cells and retinal tissue. In this study fibrin gel was used as hydrogel scaffold for immobilization of cells. The structural characteristics of fibrin gel scaffold were examined with SEM. Rheological properties of fibrin gel were measured by rheometer and biodegradation rate of fibrin were assayed for 2 weeks. After isolation of stem cells CJMSCs, the cells were differentiated into photoreceptor-like cells by exposing with taurin for 14 days in tissue culture plate (TCP group) and fibrin hydrogel (3 D group). The attachment of cells was analyzed with SEM and MTT. The expression of rhodopsin, PKC, CRX, recoverin, peripherin, nestin and RPE65 as photoreceptor-like cell markers was evaluated by immunocytochemistry and quantitative real-time PCR (RT-PCR) in TCP and 3 D groups. The results of SEM analysis showed CJMSCs were well attached in fibrin gels and there were good integrity between cells and scaffold. The elastic modulus and constant degradation of the gel contributes to the growth and proliferation of cells. There was no toxicity effect of fibrin hydrogel on cells and the viability of cultured cells was higher in 3 D fibrin gels in comparison with TCP groups. After 2 weeks, the expression of rhodopsin, PKC, CRX, peripherin, recoverin, nestin and RPE65 as special markers of photoreceptor cells were detected by Real time PCR and immunofluorescence that these expressions in 3 D groups were higher than TCP groups. In conclusion, our findings showed that application of readily available sources of adult stem cells like human conjunctiva stem cells encapsulated in fibrin gel could be interesting strategy to enhance photoreceptor progenitor cell numbers for repair and regeneration of retina disease such as photoreceptor injury.

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Section: Introductionmentioning

confidence: 99%

Fibrin gel as a scaffold for photoreceptor cells differentiation from conjunctiva mesenchymal stem cells in retina tissue engineering

Soleimannejad

Ebrahimi‐Barough

Soleimani

et al. 2017

Artificial Cells, Nanomedicine, and Biotechnology

Self Cite

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“…hEnSCs can be differentiated into Schwann cells (SCs) in both 2D and 3D cultures. These differentiated cells in fibrin gel could present new opportunities for tissue engineering approaches and subsequent treatment of neurodegenerative disorders (89). hEnSCs can differentiate into oligodendrocyte progenitors with characteristic oligodendroglial precursor cells (OPCs) morphology, and express markers such as PDGFRα, Sox10, A2B5, Olig2, and O4 (90).…”

Section: Endometrial Stem Cellsmentioning

confidence: 99%

Stem Cell Therapy: A Promising Therapeutic Approach for Multiple Sclerosis

Pourabdolhossein

Hamidabadi

Bojnordi

et al. 2017

Multiple Sclerosis: Perspectives in Treatment and Pathogenesis

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Multiple sclerosis (MS) is an inflammatory disease of the central nervous system which is accompanied by demyelination of the nerves, axonal loss, and disability. Currently, no definitive treatment is recognized for MS. Stem-cell therapy for MS has shown promising results and has attracted attention as an alternative therapeutic option. Various stem cell sources such as mesenchymal, embryonic, and neural have been identified. This chapter gives an overview of the advances made in our understanding of these stem cells under two broad categories: exogenous and endogenous. Stem-cell therapy in MS and the substantial literature regarding their

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Retina tissue engineering by conjunctiva mesenchymal stem cells encapsulated in fibrin gel: Hypotheses on novel approach to retinal diseases treatment

et al. 2017

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Human Endometrial Stem Cells May Differentiate into Schwann Cells in Fibrin Gel as 3D Culture

Cited by 3 publications

References 32 publications

Fibrin gel as a scaffold for photoreceptor cells differentiation from conjunctiva mesenchymal stem cells in retina tissue engineering

Fibrin gel as a scaffold for photoreceptor cells differentiation from conjunctiva mesenchymal stem cells in retina tissue engineering

Stem Cell Therapy: A Promising Therapeutic Approach for Multiple Sclerosis

Retina tissue engineering by conjunctiva mesenchymal stem cells encapsulated in fibrin gel: Hypotheses on novel approach to retinal diseases treatment

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