Human eyelid adipose tissue-derived Schwann cells promote regeneration of a transected sciatic nerve

Abstract

Schwann cells (SCs) can promote the regeneration of injured peripheral nerves while the clinical application is limited by donor site complications and the inability to generate an ample amount of cells. In this study, we have isolated human eyelid adipose-derived Schwann cells (hE-SCs) from human eyelid adipose tissue and identified the cell phenotype and function. Using immunofluorescence and H & E staining, we detected subtle nerve fibers and SCs in human eyelid adipose tissue. Immunofluorescence staining indicated that hE-SCs expressed glial markers, such as S100, p75NTR GFAP, Sox10 and Krox20. To explore whether hE-SCs promote the regeneration of injured peripheral nerves in vivo, a Balb/c-nu mice model was used in the study, and mice were randomly assigned to five groups: Matrigel; hE-SCs/P0; hE-SCs/P2; hE-FLCs/P2; and Autograft. After 12 weeks, functional and histological assessments of the regenerated nerves showed that sciatic nerve defect was more effectively repaired in the hE-SCs/P2 group which achieved 66.1 ± 6.5% purity, than the other three groups and recovered to similar level to the Autograft group. These results indicated that hE-SCs can promote the regeneration of injured peripheral nerve and the abundant, easily accessible supply of adipose tissue might be a promising source of SCs for peripheral nerve repair.

Monocarboxylate transporter 1 in Schwann cells contributes to maintenance of sensory nerve myelination during aging

“…GLUTs in endothelial cells (Froehner, Davies, Baldwin, & Lienhard, 1988), perineurial cells (Gerhart & Drewes, 1990;Takebe et al, 2008), and SCs (Magnani et al, 1996) (Jessen & Mirsky, 2008). p75 NTR , which has been implicated in the regulation of gene expression, cell cycle transitions, and myelination by recruiting specific binding proteins, is a marker for immature SCs that is highly expressed primarily during development and after nerve injury (Cragnolini & Friedman, 2008;Wang et al, 2017). Though c-Jun is also upregulated by mitochondrial injury, the upregulation of p75 NTR confirms the presence of increased immature SCs in P0-MCT1 KO mice.…”

Monocarboxylate transporter 1 in Schwann cells is critical for maintenance of sensory nerve myelination during aging

“…c-Jun is a negative regulator of SC differentiation, myelination, and has been implicated in demyelinating neuropathies (Jessen & Mirsky, 2008). p75 NTR , which has been implicated in the regulation of gene expression, cell cycle transitions, and myelination by recruiting specific binding proteins, is a marker for immature SCs that is highly expressed primarily during development and after nerve injury (Cragnolini & Friedman, 2008;Wang et al, 2017). Though c-Jun is also upregulated by mitochondrial injury, the upregulation of p75 NTR confirms the presence of increased immature SCs in P0-MCT1 KO mice.…”

Comunicação exossomal na transdiferenciação de células-tronco em cocultivo com células neuronais

“…Um outro aspecto que deve ser considerado para caracterização das ADSC e outras fontes de células-tronco mesenquimais é a capacidade de diferenciação em linhagens celulares mesodérmica como osteoblastos (HANADA et al, 1997), condrócitos (TECHNAU et al, 2011) e adipócitos (DU et al, 2016GIMBLE;GUILAK, 2003;), assim como linhagens celulares ectodérmica como neurônios (ABDANIPOUR; TIRAIHI, 2012;DHANASEKARAN et al, 2013;FRANCO LAMBERT et al, 2009;HUANG et al, 2007;KURIHARA et al, 2014), células da glia (ZAVAN et al, 2010) e células de Schwann (LIU, F. et al, 2013;WANG et al, 2017;XU et al, 2008 A aplicabilidade terapêutica é um ponto importante a ser considerado quando se trabalha com células-tronco como terapia regenerativa. No caso das ADSC já foi demonstrado por Cooney et al (2016), De Lombardo et al (2009) e Delarosa e Lombardo (2010) sua capacidade de modulação e atuação no processo de regeneração em lesões de nervo periférico.…”

“…A principal consequência da lesões de nervo periférico (PNI -sigla em inglês para peripheral nerve injury) é a perda definitiva da função do membro ou local afetado, sendo que muitas vezes o uso de transplante de nervo do próprio paciente, assim como a utilização de biomateriais em conjunto ou não com células-tronco, se tornam uma alternativa na busca da regeneração e retorno da função (BUENO et al, 2017;COONEY et al, 2016;GÄRTNER et al, 2014;HEI et al, 2017;MARCONI et al, 2012;ZHANG, T. et al, 2014;WANG et al, 2010;WANG et al, 2017).…”

“…Adicionalmente, em concordância com os achados apresentados neste estudo, pesquisadores utilizaram outros tipos celulares, no caso, células de Schwann humana derivadas do tecido adiposo da pálpebra em lesões de nervo periféricos reparadas com tubo de silicone, e avaliaram a funcionalidade pelo peso do músculo Gastrocnêmio e histologia do nervo reparado após 12 semanas (WANG et al, 2017).…”