Human fallopian tube epithelial cells exhibit stemness features, self-renewal capacity, and Wnt-related organoid formation

Chang, Yu-Hsun; Chu, Tang-Yuan; Ding, Dah‐Ching

doi:10.1186/s12929-019-0602-1

Cited by 50 publications

(35 citation statements)

References 38 publications

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“…The existence of adult stem/progenitor cells (ASCs) has been reported in the oviduct of humans [ 73 ] and mice [ 74 ]. In both species, spherical oviduct organoids composed of fully differentiated ciliated and secretory OECs (with their apical side towards the lumen) have been successfully generated from sorted and Matrigel © -embedded ASCs [ 73 , 75 , 76 ]. Oviduct organoids can reproduce the in-vivo-like folds of the oviduct epithelium and can be maintained long-term (> 16 months) in culture [ 73 ].…”

Section: Three-dimensional Models Of the Oviduct For The Study Of Thementioning

confidence: 99%

“…Oviductal organoids in humans and mice have mostly been used as models to study the origin and development of epithelial ovarian cancers, as the fallopian tube has been implicated as a site of origin of aggressive cancers [ 73 , 75 , 76 ]. In farm animal species, new physiological in vitro 3D models of the oviductal epithelium are needed to understand better the action of systemic regulators (e.g., ovarian steroid hormones) and local cellular interactions with gametes and embryos during the periconception period.…”

Section: Three-dimensional Models Of the Oviduct For The Study Of Thementioning

confidence: 99%

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Progress and challenges in developing organoids in farm animal species for the study of reproduction and their applications to reproductive biotechnologies

Bourdon

Cadoret

Charpigny

et al. 2021

Vet Res

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Within the past decades, major progress has been accomplished in isolating germ/stem/pluripotent cells, in refining culture medium and conditions and in establishing 3-dimensional culture systems, towards developing organoids for organs involved in reproduction in mice and to some extent in humans. Haploid male germ cells were generated in vitro from primordial germ cells. So were oocytes, with additional support from ovarian cells and subsequent follicle culture. Going on with the female reproductive tract, spherical oviduct organoids were obtained from adult stem/progenitor cells. Multicellular endometrial structures mimicking functional uterine glands were derived from endometrial cells. Trophoblastic stem cells were induced to form 3-dimensional syncytial-like structures and exhibited invasive properties, a crucial point for placentation. Finally, considering the embryo itself, pluripotent embryonic cells together with additional extra-embryonic cells, could self-organize into a blastoid, and eventually into a post-implantation-like embryo. Most of these accomplishments have yet to be reached in farm animals, but much effort is devoted towards this goal. Here, we review the progress and discuss the specific challenges of developing organoids for the study of reproductive biology in these species. We consider the use of such organoids in basic research to delineate the physiological mechanisms involved at each step of the reproductive process, or to understand how they are altered by environmental factors relevant to animal breeding. We evaluate their potential in reproduction of animals with a high genetic value, from a breeding point of view or in the context of preserving local breeds with limited headcounts.

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Section: Three-dimensional Models Of the Oviduct For The Study Of Thementioning

confidence: 99%

Section: Three-dimensional Models Of the Oviduct For The Study Of Thementioning

confidence: 99%

Progress and challenges in developing organoids in farm animal species for the study of reproduction and their applications to reproductive biotechnologies

Bourdon

Cadoret

Charpigny

et al. 2021

Vet Res

View full text Add to dashboard Cite

show abstract

“…Conditioned medium obtained after 24 hours was analyzed for IL-6 levels using ELISA. Cell proliferation of IL-6 overexpressing cells was carried out by XTT, as previously described (Chang et al, 2020). Cells were grown in Roswell Park Memorial Institute medium (RPMI, SigmaAldrich) and supplemented with 1.5% non-essential amino acids, 0.75 % MEM vitamins and 1.5 % sodium bicarbonate.…”

Section: Methodsmentioning

confidence: 99%

Tumor-educated monocyte-dendritic progenitors promote a metastatic switch

K¹,

Kveler

et al. 2020

Preprint

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Myeloid skewing of hematopoietic cells is a prominent promoter of metastasis. However, the reservoir of these cells in the bone marrow (BM) compartment, their education, immune memory and their differentiation pattern from uncommitted hematopoietic stem cells (HSCs) have not been explored. Here we show that metastatic tumors dictate a unique differentiation pattern of uncommitted HSCs towards myeloid progeny. Single cell RNA-sequencing analysis integrated with proteomic screen of tumor secretome revealed that HSCs differentiate into monocyte-dendritic progenitors (MDP) in highly metastatic tumors but not in low-metastatic tumors which display HSC differentiation into granulocyte-monocyte progenitors (GMP). This effect is driven by IL-6/IL-6R axis which is highly active in metastatic tumors. Consequently, loss and gain of function of IL-6 in tumor cells resulted in decreased and increased metastasis and corresponding MDP levels, respectively. Consistently, MDPs but not GMPs obtained from highly metastatic tumors, adoptively transferred into mice bearing tumors resulted in increased metastasis. Our study reveals a unique tumor-BM crosstalk that dictates a specific long-lived education of HSCs towards myeloid differentiation, thereby promoting the enrichment of metastasis-associated immune cells in the tumor microenvironment.

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“…After deriving fallopian tube epithelial cells from the epithelial monolayer of the human fallopian tube epithelium, Chang et al observed a formation of spheroids in gelatin-coated cultures. Downstream analysis of these spheroids revealed expression of SSEA3 and SSEA4 [ 64 ]. Abundant SSEA4 expression was also noted in the epithelium of benign serous cystadenoma with a gradual decrease towards advanced tumor stage [ 65 ].…”

Section: Globo-series Gslsmentioning

confidence: 99%

Deciphering the Importance of Glycosphingolipids on Cellular and Molecular Mechanisms Associated with Epithelial-to-Mesenchymal Transition in Cancer

et al. 2021

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Every living cell is covered with a dense and complex layer of glycans on the cell surface, which have important functions in the interaction between cells and their environment. Glycosphingolipids (GSLs) are glycans linked to lipid molecules that together with sphingolipids, sterols, and proteins form plasma membrane lipid rafts that contribute to membrane integrity and provide specific recognition sites. GSLs are subdivided into three major series (globo-, ganglio-, and neolacto-series) and are synthesized in a non-template driven process by enzymes localized in the ER and Golgi apparatus. Altered glycosylation of lipids are known to be involved in tumor development and metastasis. Metastasis is frequently linked with reversible epithelial-to-mesenchymal transition (EMT), a process involved in tumor progression, and the formation of new distant metastatic sites (mesenchymal-to-epithelial transition or MET). On a single cell basis, cancer cells lose their epithelial features to gain mesenchymal characteristics via mechanisms influenced by the composition of the GSLs on the cell surface. Here, we summarize the literature on GSLs in the context of reversible and cancer-associated EMT and discuss how the modification of GSLs at the cell surface may promote this process.

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Human fallopian tube epithelial cells exhibit stemness features, self-renewal capacity, and Wnt-related organoid formation

Cited by 50 publications

References 38 publications

Progress and challenges in developing organoids in farm animal species for the study of reproduction and their applications to reproductive biotechnologies

Progress and challenges in developing organoids in farm animal species for the study of reproduction and their applications to reproductive biotechnologies

Tumor-educated monocyte-dendritic progenitors promote a metastatic switch

Deciphering the Importance of Glycosphingolipids on Cellular and Molecular Mechanisms Associated with Epithelial-to-Mesenchymal Transition in Cancer

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