Human Fetal Antibody-Dependent Cellular Cytotoxicity to Herpes Simplex Virus–Infected Cells

Landers, Daniel V.; Smith, Janis P; Walker, Cheryl K.; Milam, Terry D.; Sanchez-Pescador, Lisa; Kohl, Steve

doi:10.1203/00006450-199403000-00001

Cited by 5 publications

(3 citation statements)

References 7 publications

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“…Several defects have been identified in NK cells of newborn infants, and these defects may make newborn infants particularly more susceptible to viral infections than adults (1,11,24,27,47,49,52). NK cell cytolytic function, as measured by the level of killing of K562 cells, is decreased about 50% in newborns compared to that in adults (25), and neonatal NK cells have a decreased ability to kill cells infected with herpesvirus (18,21). Newborn mononuclear cells (MNCs) have decreased antibody-dependent cellular cytotoxicity (ADCC) compared to the ADCC of MNCs from adults (11,12), and newborns have decreased numbers of NK cells with both CD16 and CD56, a phenotype associated with greater cytotoxicity (32).…”

mentioning

confidence: 99%

Enhancement of Antibody-Dependent Cellular Cytotoxicity of Neonatal Cells by Interleukin-2 (IL-2) and IL-12

Nguyen

Roberts

Ank

et al. 1998

Clin Diagn Lab Immunol

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Newborn infants are more susceptible to infections due in part to deficiencies in the cytotoxic functions of their lymphocytes. We investigated the ability of interleukin-2 (IL-2) and IL-12 to enhance the cytotoxicity of neonatal (cord blood) and adult mononuclear cells (MNCs) in both natural killer (NK) cell and antibody-dependent cellular cytotoxicity (ADCC) assays. The cytotoxic activity of cord blood MNCs was less than 50% that of adult MNCs in most assays prior to exposure to cytokines. Incubation with IL-2 (100 U/ml) or IL-12 (1 ng/ml) for 18 h increased the NK cell activity (using K562 target cells) of both cord blood and adult MNCs, and the combination of IL-2 and IL-12 increased cord blood cytotoxicity threefold, making the cytotoxicity of cord blood cells equivalent to that of adult cells treated with the same cytokines. In ADCC assays with chicken erythrocyte targets, the combination of IL-2 and IL-12 increased the cytotoxicities of both cord blood and adult MNCs, with greater enhancement again seen with cord blood cells. In assays with NK cell-resistant CEM cells coated with human immunodeficiency virus (HIV) gp120 antigen in the presence of hyperimmune anti-HIV immunoglobulin, ADCC of cord blood MNCs was about 50% that of adult MNCs; ADCC of cord blood MNCs increased two- to threefold with the addition of IL-2 and IL-12, whereas ADCC of adult MNCs did not increase. Incubation of cord blood cells, but not adult cells, with IL-2 or IL-12 for 1 week increased the percentage of CD16+/CD56+ cells two- to fivefold and enhanced ADCC activity. Thus, IL-2 and IL-12 greatly enhance both the NK cell and ADCC activities of neonatal MNCs and increase the number of NK cells in longer-term culture.

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mentioning

confidence: 99%

Enhancement of Antibody-Dependent Cellular Cytotoxicity of Neonatal Cells by Interleukin-2 (IL-2) and IL-12

Nguyen

Roberts

Ank

et al. 1998

Clin Diagn Lab Immunol

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“…Antibody-dependent cellular cytotoxicity (ADCC), mediated by NK cells, is lower in newborn mononuclear cells (MNC) than in adults (27,28,30). MNC from newborn infants have reduced capacity to produce cytokines, particularly interleukin-2 (IL-2) and gamma interferon (12,14,30,31), both of which are important in upregulating NK cytotoxicity, which further contributes to the newborn's susceptibility to infection (32,34,35).…”

mentioning

confidence: 99%

Interleukin-15 Enhances Cytotoxicity, Receptor Expression, and Expansion of Neonatal Natural Killer Cells in Long-Term Culture

Choi

Chhabra

Nguyen

et al. 2004

Clin Vaccine Immunol

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“…ADCC has extensively been studied in the context of HIV infections in which the interaction of anti-HIV Abs with NK cells offers protection and even predicts viral load and clinical outcome [71, 72]. ADCC against herpes simplex virus and HIV is decreased in neonates compared to adults possibly reflecting, in part, deficient interaction of matAbs and NK cells in the neonatal period [73–75]. …”

Section: The Effect Of Fc Gamma Receptors On Innate Immunitymentioning

confidence: 99%

Fc gamma receptors in respiratory syncytial virus infections: implications for innate immunity

et al. 2013

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SUMMARY Respiratory syncytial virus infections are a major burden in infants less than 3 months of age. Newborns and infants express a distinct immune system that is largely dependent on innate immunity and passive immunity from maternal antibodies. Antibodies can regulate immune responses against viruses through interaction with Fc gamma receptors leading to enhancement or neutralization of viral infections. The mechanisms underlying the immunomodulatory effect of Fc gamma receptors on viral infections have yet to be elucidated in infants. Herein, we will discuss current knowledge of the effects of antibodies and Fc gamma receptors on infant innate immunity to RSV. A better understanding of the pathogenesis of RSV infections in young infants may provide insight into novel therapeutic strategies like vaccination.

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Human Fetal Antibody-Dependent Cellular Cytotoxicity to Herpes Simplex Virus–Infected Cells

Cited by 5 publications

References 7 publications

Enhancement of Antibody-Dependent Cellular Cytotoxicity of Neonatal Cells by Interleukin-2 (IL-2) and IL-12

Enhancement of Antibody-Dependent Cellular Cytotoxicity of Neonatal Cells by Interleukin-2 (IL-2) and IL-12

Interleukin-15 Enhances Cytotoxicity, Receptor Expression, and Expansion of Neonatal Natural Killer Cells in Long-Term Culture

Fc gamma receptors in respiratory syncytial virus infections: implications for innate immunity

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