Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine

Tomar, Geetanjali B.; Srivastava, Rupesh K.; Gupta, Navita; Barhanpurkar, Amruta P.; Pote, Satish T.; Jhaveri, Hiral M.; Mishra, Gyan C.; Wani, Mohan R.

doi:10.1016/j.bbrc.2010.01.126

Cited by 307 publications

(336 citation statements)

References 25 publications

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“…To assess the clonogenic potential of ASCs, a CFU-fibroblast (CFU-F) assay was performed as described previously (21). Briefly, 1 3 10 3 cells were cultured in a 60-mm petri dish for 7-10 d followed by formalin fixation and staining with hematoxylin.…”

Section: Cfu-fibroblast Assaymentioning

confidence: 99%

“…In vitro osteogenic, adipogenic, and chondrogenic differentiation of ASCs Induction of osteoblast, adipocyte, and chondrocyte differentiation was performed as described previously (21). For osteoblast differentiation, monolayer cultures of mouse ASCs (2-5 3 10 3 cells/cm 2 ) were incubated in a-MEM containing 10% FCS, 10 mM b-glycerophosphate, 50 mg/ml ascorbic acid, and 100 nM dexamethasone for 21-25 d. Cultures were half fed every third day and assessed for matrix mineralization by Alizarin red S staining.…”

Section: Cfu-fibroblast Assaymentioning

confidence: 99%

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Adipose-Derived Mesenchymal Stem Cells Prevent Systemic Bone Loss in Collagen-Induced Arthritis

Garimella

Kour

Piprode

et al. 2015

The Journal of Immunology

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Rheumatoid arthritis (RA) is an autoimmune disease characterized by chronic inflammatory synovitis leading to joint destruction and systemic bone loss. The inflammation-induced bone loss is mediated by increased osteoclast formation and function. Current antirheumatic therapies primarily target suppression of inflammatory cascade with limited or no success in controlling progression of bone destruction. Mesenchymal stem cells (MSCs) by virtue of their tissue repair and immunomodulatory properties have shown promising results in various autoimmune and degenerative diseases. However, the role of MSCs in prevention of bone destruction in RA is not yet understood. In this study, we investigated the effect of adipose-derived MSCs (ASCs) on in vitro formation of bone-resorbing osteoclasts and pathological bone loss in the mouse collagen-induced arthritis (CIA) model of RA. We observed that ASCs significantly inhibited receptor activator of NF-κB ligand (RANKL)–induced osteoclastogenesis in both a contact-dependent and -independent manner. Additionally, ASCs inhibited RANKL-induced osteoclastogenesis in the presence of proinflammatory cytokines such as TNF-α, IL-17, and IL-1β. Furthermore, treatment with ASCs at the onset of CIA significantly reduced clinical symptoms and joint pathology. Interestingly, ASCs protected periarticular and systemic bone loss in CIA mice by maintaining trabecular bone structure. We further observed that treatment with ASCs reduced osteoclast precursors in bone marrow, resulting in decreased osteoclastogenesis. Moreover, ASCs suppressed autoimmune T cell responses and increased the percentages of peripheral regulatory T and B cells. Thus, we provide strong evidence that ASCs ameliorate inflammation-induced systemic bone loss in CIA mice by reducing osteoclast precursors and promoting immune tolerance.

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Section: Cfu-fibroblast Assaymentioning

confidence: 99%

Section: Cfu-fibroblast Assaymentioning

confidence: 99%

Adipose-Derived Mesenchymal Stem Cells Prevent Systemic Bone Loss in Collagen-Induced Arthritis

Garimella

Kour

Piprode

et al. 2015

The Journal of Immunology

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“…These results agree with other studies devoted to gingival MMSC that reported similar phenotype characteristics, differentiation potential and stable genomic behavior. 19,39,40 Also, we determined high proliferative capacity of AMC which remained constant from primary to long-term cultures. It should be noted that the population doubling time for bone marrowderived MMSC reaches only 55 § 3 hours under similar cultivation conditions.…”

Section: Discussionmentioning

confidence: 99%

“…26,27 Moreover, the amount of MMSC able to multipotent differentiation is higher in gingival mucosa compared to other tissues. 28,29,30 In primary cultures of gingival fibroblasts up to 90% of cells possess "stemness" markers and 40-70% of cells express pluripotency genes such as Oct4, Sox2, Nanog 28 and active telomerase. 29 It is well known that gingiva can be visually divided into several zones: free gingiva, attached gingiva, alveolar mucosa etc.…”

Section: Introductionmentioning

confidence: 99%

Myogenic potential of human alveolar mucosa derived cells

et al. 2017

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Difficulties related to the obtainment of stem/progenitor cells from skeletal muscle tissue make the search for new sources of myogenic cells highly relevant. Alveolar mucosa might be considered as a perspective candidate due to availability and high proliferative capacity of its cells. Human alveolar mucosa cells (AMC) were obtained from gingival biopsy samples collected from 10 healthy donors and cultured up to 10 passages. AMC matched the generally accepted multipotent mesenchymal stromal cells criteria and possess population doubling time, caryotype and immunophenotype stability during long-term cultivation. The single myogenic induction of primary cell cultures resulted in differentiation of AMC into multinucleated myotubes. The myogenic differentiation was associated with expression of skeletal muscle markers: skeletal myosin, skeletal actin, myogenin and MyoD1. Efficiency of myogenic differentiation in AMC cultures was similar to that in skeletal muscle cells. Furthermore, some of differentiated myotubes exhibited contractions in vitro. Our data confirms the sufficiently high myogenic potential and proliferative capacity of AMC and their ability to maintain in vitro proliferation-competent myogenic precursor cells regardless of the passage number.

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“…Compared to BM-MSCs, these cells have a further advantage to be easier to obtain, using less invasive procedures, without risks to the donor, and ethically free. Therefore, they represent a new niche of stem cells for regenerative medicine (Trubiani et al 2008;Tomar et al 2010). Many studies have reported the in vitro formation of neuronal lineage cells from PDLSCs (Kadar et al 2009;Osathanon et al 2013).…”

Section: Dental Tissues-derived Mscsmentioning

confidence: 99%

The transplantation of mesenchymal stem cells derived from unconventional sources: an innovative approach to multiple sclerosis therapy

Giacoppo

Bramantı

Mazzon

2017

Arch. Immunol. Ther. Exp.

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Human gingiva-derived mesenchymal stem cells are superior to bone marrow-derived mesenchymal stem cells for cell therapy in regenerative medicine

Cited by 307 publications

References 25 publications

Adipose-Derived Mesenchymal Stem Cells Prevent Systemic Bone Loss in Collagen-Induced Arthritis

Adipose-Derived Mesenchymal Stem Cells Prevent Systemic Bone Loss in Collagen-Induced Arthritis

Myogenic potential of human alveolar mucosa derived cells

The transplantation of mesenchymal stem cells derived from unconventional sources: an innovative approach to multiple sclerosis therapy

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