Human growth hormone but not ovine or bovine growth hormones exhibits galactopoietic prolactin-like activity in organ culture from bovine lactating mammary gland

Gertler, Arieh; Maoz, Arieh

doi:10.1016/0303-7207(83)90165-x

Cited by 79 publications

(36 citation statements)

References 20 publications

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“…These factors show the same competition pattern, are recognized by the same antiserum NIS93, and comigrate with MGF. Furthermore, hGH is known to be a ligand for PRL-R and to induce lactation in the rabbit, whereas bGH shows only a low affinity for rabbit PRL-R with little lactogenic activity (54,55). Thus, we suggest that hGH, and to a much lesser degree bGH, interact with the rabbit PRL-R to activate MGF.…”

Section: Figmentioning

confidence: 77%

“…Human GH and oPRL bind the rabbit PRL-R with high affinity and exhibit a potent lactogenic activity in these cells. However, bovine GH is considered as totally devoid of PRL-like activity in explants from bovine mammary gland and has a very low affinity for recombinant rabbit PRL-R (54,55). 3 Cells were treated by oPRL, pIFN␥, hGH, and bGH for 15 min.…”

Section: Fig 4 Induction Of Two Different ␤-Cas Gas-binding Complexmentioning

confidence: 99%

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Activation of STAT Factors by Prolactin, Interferon-γ, Growth Hormones, and a Tyrosine Phosphatase Inhibitor in Rabbit Primary Mammary Epithelial Cells

Schindler

Larose

et al. 1995

Journal of Biological Chemistry

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In numerous studies on mammary epithelial cell lines multiple factors, added to the medium or contained in the serum, were required for casein gene expression. It has been shown in these systems that the mammary gland factor (MGF) is implicated in the activation of the ␤-casein gene promoter. In the present study, we determined the relationship between known agents that affect casein gene expression and MGF activity using the properties of rabbit primary mammary epithelial cells to respond to PRL alone, when cultured in chemically defined medium. We demonstrate that MGF is rapidly activated by PRL alone or by human growth hormone, a natural ligand of many PRL receptors (PRL-Rs), in the cytoplasm and accumulated in the nucleus. The MGF activation by PRL occurred in the absence of endogenous extracellular matrix, a condition where casein synthesis is known to be markedly reduced. Different inhibitors of protein-tyrosine kinases, which have been shown to reduce casein mRNA synthesis, but not of protein kinase C, decrease the MGF activity. A tyrosine phosphatase inhibitor, sodium pervanadate, induced two GAS-binding complexes related to MGF and STAT1. Our data show that MGF is a latent cytoplasmic factor rapidly activated in mammary epithelial cells, by a mechanism involving a tyrosine kinase and a tyrosine phosphatase.

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Section: Figmentioning

confidence: 77%

Section: Fig 4 Induction Of Two Different ␤-Cas Gas-binding Complexmentioning

confidence: 99%

Activation of STAT Factors by Prolactin, Interferon-γ, Growth Hormones, and a Tyrosine Phosphatase Inhibitor in Rabbit Primary Mammary Epithelial Cells

Schindler

Larose

et al. 1995

Journal of Biological Chemistry

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“…The combination of increased turnover and up-regulation of the highaffinity GH receptor in response to elevated blood GH may be one of the key events leading to the well-documented galactopoietic properties of injected rbGH (Gluckman and Breier 1989 (Gertler et al 1983;Goodman et al 1983). Other in vitro experiments revealed subtle changes in the production of some milk constituents but this may have been due to prolactin contamination of the GH preparations used (Nandi et al 1982;Skarda et al 1982 There is also evidence that rbGH has a direct action on marnmary cells.…”

Section: Effects Of Growth Hormone On Body Conditionmentioning

confidence: 99%

A review of bovine growth hormone

Burton¹,

McBride²,

Block³

et al. 1994

Can. J. Anim. Sci.

132

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. 74: 16'7-201. Unprecedented numbers of technical papers, abstracts, and short communications have been published in the past decade regarding the effects of exogenous bovine growth hormone on milk production, health, and reproductive efficiency of treated dairy cows. In well-managed dairy herds, exogenous growth hormone increases milk production without altering normal variability in milk composition. This has held true regardless of dairy breed tested, geographical location studied, or feeding management system used. Also consistent across studies is the rapidity of the galactopoietic effect of administered bovine growth hormone, which arises from altered partitioning and use of post-absorptive nutrients and increased synthetic capacity of the mammary gland. Growth hormone and its associated peptide, insulin-like growth factor-I, are now known to provide chronic lipolytic, diabetogenic, and gluconeogenic signals to target tissues culminating in increased mammary gland availability of glucose and nonesterified fatty acids. Together with yet ill-defined effects on mammary secretory tissue, this homeorhetic control of metabolism elicited by exogenous growth hormone is so efficient that treated cows are not more susceptible to metabolic disorders than untreated cows. However, some studies have reported an increased frequency of mastitis in groups of treated cows. This has been attributed mainly to increased milk volume in the mammary glands of treated cows and no convincing data are available that show decreased mammary gland immunity as a result of growth hormone treatments. On the contrary, an expanding body of evidence implicates growth hormone as a key neuroendocrine factor that is required for immunological competence. Trends of decreased reproductive efficiency in cows treated with growth hormone have also been reported, but available data imply that this is probably an indirect effect via prolonged negative energy balance in cows treated in early lactation rather than a direct negative effect on estrous cycling via altered reproductive hormone profiles. The objectives of the present review are to bring into focus and summarize pertinent biological discoveries regarding the treatment of dairy cows with recombinant bovine growth hormone, and to explore areas where additional growth hormone research is needed or warranted.Key words: Growth hormone, somatotropin, dairy cows, insulin-like growth factor-I Burton, J. L., McBride, B. W., Block, E., Glimm, D. et Kennelly,E. 1994. L'horrnone de croissance bovinemais au point bibliographique. Can. J. Anim. Sci. 74: . D'innombrables m6moires techniques, r6sum6s analytiques et communications brdves ont 6t6 publi6s dans la dernidre d6cennie au sujet des effets de I'hormone de croissance bovine exogdne sur la production laitidre, sur la sant6 et sur les fonctions de reproduction des vaches laitidres trait6es. Dans les 6levages laitiers bien conduits, la GH exogbne accroit la production de lait sans modifier la variabilit6 normale de sa composition, et cela inddpendamme...

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“…Four hours later, the well contents were transferred to Eppendoff tuh~s and counted in a gamma counter. Iodination of hGH and bPL was carried out as described previously [17],.…”

Section: Binding Assaysmentioning

confidence: 99%

Biological activity of bovine placental lactogen in 3T3‐F442A preadipocytes is mediated through a somatogenic receptor

Vashdi¹,

Elberg²,

Sakal³

et al. 1992

FEBS Letters

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Bovine placental lactogen (bPL) exhibited antimitogenic differentiation‐promoting, biological activity in 3T3‐F442A preadipocytes, Competitive binding studies and affinity labelling revealed bPL activity to be mediated through a somatogenic type of receptor that recognizes human growth hormone (hGH) and bovine GH, but not ovine prolactin or human PL. The bioactivity of bPL was sixfold lower than that of hGH despite that bPL is binding to the somatogenic receptors with fivefold higher affinity. This discrepancy may result from the relatively low ability of bPL to induce post‐receptoral effects such as receptor dimerization.

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Human growth hormone but not ovine or bovine growth hormones exhibits galactopoietic prolactin-like activity in organ culture from bovine lactating mammary gland

Cited by 79 publications

References 20 publications

Activation of STAT Factors by Prolactin, Interferon-γ, Growth Hormones, and a Tyrosine Phosphatase Inhibitor in Rabbit Primary Mammary Epithelial Cells

Activation of STAT Factors by Prolactin, Interferon-γ, Growth Hormones, and a Tyrosine Phosphatase Inhibitor in Rabbit Primary Mammary Epithelial Cells

A review of bovine growth hormone

Biological activity of bovine placental lactogen in 3T3‐F442A preadipocytes is mediated through a somatogenic receptor

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