Human hepatic myofibroblasts increase invasiveness of hepatocellular carcinoma cells: Evidence for a role of hepatocyte growth factor

Abstract

RT-PCR and Western blot analysis confirmed thatAlso, tumor-associated MF have been shown to be involved both tumor cell lines expressed c-met, the receptor for HGF.in the in vivo and in vitro invasiveness of colon cancer cell The effects of MF-conditioned medium were not reproduced lines. 6 by acidic fibroblast growth factor, basic fibroblast growth Hepatocyte growth factor (HGF) is a multifunctional cytofactor, epidermal growth factor (EGF), transforming growth kine that has been implicated in the pathogenesis of several factor-b1 (TGF-b1), or platelet-derived growth factor (PDGFtumors. It acts on the cells via its specific receptor, c-met. 7 BB). Reverse transcription-polymerase chain reaction (RTIt has recently been shown, using in situ hybridization, that PCR) analysis confirmed that HGF was expressed in human human liver myofibroblasts express HGF in patients with HCC. Our data show that human liver MF act on HCC cells cirrhosis. 8 Although there are still some contradictory results, to increase their invasiveness and suggest that MF-derived most studies indicate that HGF is expressed in human, 9-13 or murine HCC. 14,15

Rat hepatic stellate cells alter the gene expression profile and promote the growth, migration and invasion of hepatocellular carcinoma cells

“…It was observed that iHSC-CM promoted the proliferation, migration and invasion of HCC cells in vitro , a result consistent with previous studies (9,15,16). Our in vivo results were similar.…”

Biochemical and pharmacological characterization of human c-Met neutralizing monoclonal antibody CE-355621

“…HepG2 cells are hepatocellular tumor cells that express c-Met 32 and respond to exogenous HGF by exhibiting elevated cell motility and matrigel invasion, 33,34 which coincides with suppression of cell proliferation 33 and formation of tubular to completely block ligand binding and showed subnanomolar potencies ( Fig. 1A; Table 1) that correlated well with the binding activity observed in the ECD binding ELISA and affinity measurements using Biacore ( Table 1).…”

The Role of Cancer-Associated Fibroblasts and Fibrosis in Liver Cancer

“…HGF, which is predominantly expressed in HSC and endothelial cells in the normal liver, has been detected in cirrhotic liver tissue, adenomas, and 80% of HCC cases (159). Neutralization of HSC-secreted HGF inhibits the proliferation and invasion capacity of HepG2, Huh7, Hep3b, and PLC cells in vitro (83, 160). Tumor promotion by HGF is attributed to MAPK/ERK-induced proliferation and survival signals in HCC (reviewed in 161) and activation of a c-Met/FRA1/HEY1 signaling cascade that contributes to HCC invasiveness and chemoresistance (81).…”