Human HLA class I- and HLA class II-restricted cloned cytotoxic T lymphocytes identify a cluster of epitopes on the measles virus fusion protein

Binnendijk, Robert S. van; Oosten, J P Versteeg-van; Poelen, Martien C. M.; Brugghe, Humphrey F.; Hoogerhout, P.; Osterhaus, Albert D. M. E.; Uytdehaag, Fons G. C. M.

doi:10.1128/jvi.67.4.2276-2284.1993

Cited by 46 publications

(27 citation statements)

References 58 publications

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“…RSV infected cells were used as antigen presenting cells when at least 30% of the cells were found positive. Before use in T cell stimulation experiments RSV infected and control B-LCL were UV irradiated essentially as described previously for measles infected B-LCL (302 nm, 2.5 × 10 −2 W/mm 2 ) [Van Binnendijk et al, 1993].…”

Section: Establishment and Rsv Infection Of B-lclmentioning

confidence: 99%

Type 1‐like immune response is found in children with respiratory syncytial virus infection regardless of clinical severity

Brandenburg,

Kleinjan,

van het Land

et al. 2000

J. Med. Virol.

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The immunological response of infants younger than six months to infection with respiratory syncytial virus (RSV) was studied in relation to clinical severity. IL-6 and IL-8 were found more frequently and at higher levels in the plasma samples of more severely ill patients and no significant differences were found in the levels of cytokines differentiating between Type 1 and Type 2 responses. Cellular infiltrates in nasopharyngeal washings consisted mainly of polymorphonuclear granulocytes and monocytes. Eosinophils, IgE positive cells and tryptase positive cells were found sporadically. Analyses of RSV stimulated T cell cultures established from peripheral blood mononuclear cells, for intracellular and secreted cytokines showed that, irrespective of clinical severity, the responses were dominated by the production of IFN-gamma, and that only low levels of IL-4 and IL-10 were detectable. Collectively these data do not indicate an association between clinical severity and a Type 2-like T cell response.

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Section: Establishment and Rsv Infection Of B-lclmentioning

confidence: 99%

Type 1‐like immune response is found in children with respiratory syncytial virus infection regardless of clinical severity

Brandenburg,

Kleinjan,

van het Land

et al. 2000

J. Med. Virol.

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show abstract

“…suggested as responsible for the induction of memory T cells that produce Th2-like rather than Th1-like cytokines (6,32,40). Class II-restricted CTL responses have been noted for a number of other RNA viruses, including parainfluenza and Sendai viruses (4,10,14), measles viruses (38,39), and coronavirus (19) in the family Paramyxoviridae and recently for influenza virus (13,34) in the family Orthomyxoviridae. Limiting dilution analysis of parainfluenza virus-specific CD4 ϩ and CD8 ϩ T lymphocytes from the peripheral blood of adults showed relatively high frequencies for both CD4 ϩ (1:4,700) and CD8 ϩ (1:2,500) CTLps (10).…”

Section: Discussionmentioning

confidence: 99%

“…Limiting dilution analysis of parainfluenza virus-specific CD4 ϩ and CD8 ϩ T lymphocytes from the peripheral blood of adults showed relatively high frequencies for both CD4 ϩ (1:4,700) and CD8 ϩ (1:2,500) CTLps (10). Measles virus infection in humans has been shown to induce both HLA class I-and class II-restricted CTLs specific to the transmembrane fusion glycoprotein (39). In mice, Sendai virus-specific class II-restricted T-cell responses have been investigated and defined (4,13).…”

Section: Discussionmentioning

confidence: 99%

Cytotoxic T-Lymphocyte Precursor Frequencies in BALB/c Mice after Acute Respiratory Syncytial Virus (RSV) Infection or Immunization with a Formalin-Inactivated RSV Vaccine

Tripp

Anderson

1998

J. Virol.

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A better understanding of the immune response to live and formalin-inactivated respiratory syncytial virus (RSV) is important for developing nonlive vaccines. In this study, major histocompatibility complex (MHC) class I- and II-restricted, RSV-specific cytotoxic T-lymphocyte precursor (CTLp) frequencies were determined in bronchoalveolar lavage (BAL) samples and spleen lymphocytes of BALB/c mice intranasally infected with live RSV or intramuscularly inoculated with formalin-inactivated RSV (FI-RSV). After RSV infection, both class I- and class II-restricted CTLps were detected by day 4 or 5 postinfection (p.i.). Peak CTLp frequencies were detected by day 7 p.i. The class II-restricted CTLp frequencies in the BAL following RSV infection were less than class I-restricted CTLp frequencies through day 14 p.i., during which class I-restricted CTLp frequencies remained elevated, but then declined by 48 days p.i. The frequencies of class II-restricted CTLps in the BAL were 2- to 10-fold less than those of class I-restricted CTLps. For spleen cells, frequencies of both MHC class I- and II-restricted CTLps to live RSV were similar. In contrast, class II-restricted CTLps predominated in FI-RSV-vaccinated mice. RSV challenge of vaccinated mice resulted in an increase in the frequency of class I-restricted CTLps at day 3 p.i. but did not enhance class II-restricted CTLp frequencies. These studies demonstrate differences in the CTLp response to live RSV infection compared with FI-RSV immunization and help define possible mechanisms of enhanced disease after FI-RSV immunization. In addition, these studies provide a quantitative means to address potential vaccine candidates by examining both MHC class I- and II-restricted CTLp frequencies.

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“…The importance of the cell-mediated component is therefore clear, in particular the CD8+ lymphocyte response. Studies on primates have shown that even the peak of viremia and its duration were independent of B-lymphocyte deficiency if it was associated with that of the CD8+ component [59].…”

Section: Measles and Immunitymentioning

confidence: 99%

Measles: An Overview of a Re-Emerging Disease in Children and Immunocompromised Patients

et al. 2020

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Despite the availability of a safe and effective vaccine, in 2018, around 350,000 measles cases were reported worldwide, which resulted in an estimate of 142,300 deaths from measles. Additionally, in 2017, global measles cases spiked, causing the death of 110,000 people, mostly children under the age of 5 years and immunocompromised adults. The increase in measles incidence is caused by the ongoing reduction of vaccination coverage. This event has triggered public and scientific interest. For this reason, we reviewed the pathophysiology of measles infection, focusing on mechanisms by which the virus spreads systemically through the host organism. By reaching the lymphocytes from the airways through a “trojan horse” strategy, measles induces an immunosuppression status. H and F glycoproteins, both expressed in the envelope, ensure attachment of the virus to host cells and spreading from one cell to another by binding to several receptors, as described in detail. The severity of the disease depends both on the age and underlying conditions of patients as well as the social and health context in which epidemics spread, and is often burdened by sequelae and complications that may occur several years after infection. Particular attention was paid to special groups that are more susceptible to severe or atypical measles. An overview of microbiology, symptoms, diagnosis, prevention, and treatment completes and enriches the review.

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Human HLA class I- and HLA class II-restricted cloned cytotoxic T lymphocytes identify a cluster of epitopes on the measles virus fusion protein

Cited by 46 publications

References 58 publications

Type 1‐like immune response is found in children with respiratory syncytial virus infection regardless of clinical severity

Type 1‐like immune response is found in children with respiratory syncytial virus infection regardless of clinical severity

Cytotoxic T-Lymphocyte Precursor Frequencies in BALB/c Mice after Acute Respiratory Syncytial Virus (RSV) Infection or Immunization with a Formalin-Inactivated RSV Vaccine

Measles: An Overview of a Re-Emerging Disease in Children and Immunocompromised Patients

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