Human IgG Antibody Response to Aedes Nterm-34kDa Salivary Peptide, an Epidemiological Tool to Assess Vector Control in Chikungunya and Dengue Transmission Area

Abstract

BackgroundArboviral diseases are an important public health concerns. Vector control remains the sole strategy to fight against these diseases. Because of the important limits of methods currently used to assess human exposure to Aedes mosquito bites, much effort is being devoted to develop new indicators. Recent studies have reported that human antibody (Ab) responses to Aedes aegypti Nterm-34kDa salivary peptide represent a promising biomarker tool to evaluate the human-Aedes contact. The present study aims investigate whether such biomarker could be used for assessing the efficacy of vector control against Aedes.Methodology/Principal findingsSpecific human IgG response to the Nterm-34kDa peptide was assessed from 102 individuals living in urban area of Saint-Denis at La Reunion Island, Indian Ocean, before and after the implementation of vector control against Aedes mosquitoes. IgG response decreased after 2 weeks (P < 0.0001), and remained low for 4 weeks post-intervention (P = 0.0002). The specific IgG decrease was associated with the decline of Aedes mosquito density, as estimated by entomological parameters and closely correlated to vector control implementation and was not associated with the use of individual protection, daily commuting outside of the house, sex and age. Our findings indicate a probable short-term decrease of human exposure to Aedes bites just after vector control implementation.Conclusion/SignificanceResults provided in the present study indicate that IgG Ab response to Aedes aegypti Nterm-34kDa salivary peptide could be a relevant short-time indicator for evaluating the efficacy of vector control interventions against Aedes species.

New Immuno-Epidemiological Biomarker of Human Exposure to Aedes Vector Bites: From Concept to Applications

“…aegypti and that of Ae. albopictus [ 29 ], the second principal vector of arboviruses worldwide. Indeed, IgG Ab response against the Nterm-34 kDa peptide identified in Ae.…”

“…A recent study has reported that human IgG response to the Ae. aegypti Nterm-34 kDa peptide could be used for assessing the effectiveness of VCS against Aedes [ 29 ]. During this study, specific IgG response was assessed from 102 individuals living in urban area in La Reunion Island before and after the implementation of VCS against Ae.…”

“… IgG Ab response to Nterm-34 kDa salivary peptide from individuals exposed to Ae. albopictus bites before and after vector control implementation [ 29 ]. Individual IgG Ab response (ΔOD) is presented just before (T0) and then 15, 30, and 45 days after vector control implementation.…”

“…aegypti and Ae. albopictus ) in several countries: Benin, Côte d’Ivoire, Lao PDR, and La Reunion Island [ 27 , 28 , 29 , 30 ].…”

Spatial Assessment of Contact Between Humans and Anopheles and Aedes Mosquitoes in a Medium-Sized African Urban Setting, Using Salivary Antibody–Based Biomarkers

“…To improve the surveillance and control of mosquito-borne diseases, people exposed to mosquito bites were assessed to develop immunological indicators of specific human antibody responses against salivary peptides from Aedes (Nterm-34-kDa) and Anopheles (gSG6-P1). These antibody-based biomarkers were validated by several studies as relevant tools to accurately evaluate (1) the risk of malaria [19][20][21][22][23][24] and arboviruses transmission [25,26] in several settings, including urban areas, and (2) the effectiveness of different vector control strategies [27][28][29][30]. The aim of the present study was to evaluate the spatial heterogeneity of exposure to both vectors of malaria and arboviral diseases in a northern urban area of Senegal, using antibody-based biomarkers of human exposure to Anopheles and Aedes mosquito bites.…”