Human immunodeficiency virus 1 reservoir in CD4+ T cells is restricted to certain V beta subsets.

Dobrescu, Dana; Kabak, Shara; Mehta, Kamini; Suh, Chang‐Hee; Asch, Adam S.; Cameron, Paul; Hodtsev, Andrew S.; Posnett, David N.

doi:10.1073/pnas.92.12.5563

Cited by 23 publications

(14 citation statements)

References 28 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Primers SK38 and SK39 (13) were used to detect gag. Primers DQ28 and DQ29 amplify cellular HLA-DQ␣ and were used to quantify total cellular DNA (14). PCR conditions for gag were 1 cycle at 94°C for 3 min; followed by 28 cycles at 94°C for 30 s, 60°C for 30 s, and 72°C for 30 s; with a final extension cycle at 72°C for 10 min.…”

Section: Methodsmentioning

confidence: 99%

Productive infection of double-negative T cells with HIVin vivo

Marodon

Warren

Filomio

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

Section: Methodsmentioning

confidence: 99%

Productive infection of double-negative T cells with HIVin vivo

Marodon

Warren

Filomio

et al. 1999

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

“…It is possible that this early TCRBV expansion, especially of CD4+ T cells, could be advantageous to the virus in that certain TCRBV populations might be more susceptible to viral entry. In fact, it has been documented that HIV replicates more efficiently in ‘normal’ T cells expressing TCRBV12 [14,] than in CD4T cells using other TCRBV families and a decrease in TCRBV12 cells has been observed in HIV patients in some studies [5,6]. In our HIV‐LTNP patients we were unable to demonstrate an alteration of this TCRBV segment (data not shown), but in our experiments we were looking much later in the disease process.…”

Section: Discussionmentioning

confidence: 57%

T cell receptor usage in patients with non-progressing HIV infection

Bodman‐Smith

Williams²,

Johnstone³

et al. 2002

Clinical and Experimental Immunology

View full text Add to dashboard Cite

SUMMARY It is still unclear why some patients with HIV progress more slowly than others to developing full blown AIDS. In this study using flow cytometry we have investigated the TCRBV repertoire of peripheral blood T lymphocytes in 17 long‐term non‐progressing HIV patients (LTNP) to determine if there is a biased usage of T cell receptor V gene products. Patients were identified from hospital records and entered into the study. Three colour flow cytometry was used to determine the expression of the TCRBV3S5, BV5S1, BV5S2, BV5S3, BV6S1, BV7S1, BV9, BV11, BV12, BV13, BV14, BV16, BV17, BV18, BV20, BV21S3, BV22, and BV23 by CD8 and CD4 positive cells isolated from the peripheral blood of patients and controls. Increases in the absolute numbers of CD8+ T cells expressing TCRBV2 and 8 were observed in the HIV‐LTNP population (P < 0·05 in both cases). No differences were seen in numbers of CD8+ T cells expressing other TCRBV or in any TCRBV within the CD4+ T cell popu‐lation. At follow up (1–2 years later), those patients in which CD4 levels were below 500 × 106/l were those initially found to have lower levels of TCRBV8 +ve CD8 cells. A significant increase in the absolute numbers of T cells coexpressing the gamma delta (γδ) T cell receptor and CD8 were also seen in the HIV‐LTNP patients compared with controls (P = 0·002). The increase in CD8+ T cells in the HIV‐LTNP patients may be interpreted as either an antigen specific, or group of antigen specific responses to viral antigen, or less likely a viral superantigen. A low level of TCRBV8, CD8+ T cells might be predictive of a more rapid disease progression and might indicate a protective role for this population in HIV infected patients. The increase in γδT cells bearing the CD8 coreceptor suggests a role for this cell type in the response to HIV infection.

show abstract

“…Indeed, when T cell lines expressing V/312, Vfl6.7a, V/317 and V/318 were infected with HIV-1, CD4 + V/312 § lines were consistently observed to replicate HIV-1 to a higher degree than V/36.7a lines, regardless of the donor's HLA type [54]. Also, the viral load measured in vivo in V/312 + cells (DNA PCR) was found to be significantly heavier than that found in V/36.7a cells [55]. However, HIV-positive individuals failed to reveal perturbations in the levels of circulating Vr + T cells, even late in the course of the disease, suggesting that V/312 + activated T cell subsets were not completely deleted, but could perhaps remain anergic [56][57][58].…”

Section: Sags In Hiv Infection ?mentioning

confidence: 73%