Human Immunodeficiency Virus Type 1-Derived Lentivirus Vectors Pseudotyped with Envelope Glycoproteins Derived from Ross River Virus and Semliki Forest Virus

Kahl, Christoph; Marsh, Jeanne C.; Fyffe, Joanne; Sanders, D.A.R.; Cornetta, Kenneth

doi:10.1128/jvi.78.3.1421-1430.2004

Cited by 52 publications

(49 citation statements)

References 50 publications

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“…AF009606). The SFV expression vector encoded the SFV E3 to E1 glycoproteins (Kahl et al, 2004). Pseudoparticles bearing the E1 and E2 envelope glycoproteins of HCV (HCVpp), the HA and NA of AIV (AIVpp) or the E1 and E2 envelope proteins of SFV (SFVpp) were then generated and purified as described previously (Bartosch et al, 2003a;Lavillette et al, 2006).…”

Section: Methodsmentioning

confidence: 99%

Characterization of hepatitis C virus pseudoparticles by cryo-transmission electron microscopy using functionalized magnetic nanobeads

Bonnafous

Perrault

Bihan

et al. 2010

Journal of General Virology

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Cell entry and membrane fusion of the hepatitis C virus (HCV) depend on its envelope glycoproteins E1 and E2. HCV pseudotyped particles (HCVpps) are relevant and popular models to study the early steps of the HCV life cycle. However, no structural characterization of HCVpp has been available so far. Using cryo-transmission electron microscopy (cryo-TEM), providing structural information at nanometric resolution, the molecular details of HCVpps and their fusion with liposomes were studied. Cryo-TEM revealed HCVpps as regular 100 nm spherical structures containing the dense retroviral nucleocapsid surrounded by a lipid bilayer. E1-E2 glycoproteins were not readily visible on the membrane surface. Pseudoparticles bearing the E1-E2 glycoproteins of Semliki forest virus looked similar, whereas avian influenza A virus (fowl plague virus) haemagglutinin/neuraminidase-pseudotyped particles exhibited surface spikes. To further characterize HCVpp structurally, a novel method was designed based on magnetic beads covered with anti-HCV antibodies to enrich the samples with particles containing E1-E2. This strategy efficiently sorted HCVpps, which were then directly observed by cryo-TEM in the presence or absence of liposomes at low or neutral pH. After acidification, HCVpps looked the same as at neutral pH and closely contacted the liposomes. These are the first visualizations of early HCV membrane fusion events at the nanometer scale. Furthermore, fluorimetry analysis revealed a relative resistance of HCVpps regarding their fusion capacity when exposed to low pH. This study therefore brings several new molecular details to HCVpp characterization and this efficient strategy of virion immunosorting with magnetic nanobeads is direct, efficient and adaptable to extensive characterization of any virus at a nanometric resolution.

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Section: Methodsmentioning

confidence: 99%

Characterization of hepatitis C virus pseudoparticles by cryo-transmission electron microscopy using functionalized magnetic nanobeads

Bonnafous

Perrault

Bihan

et al. 2010

Journal of General Virology

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“…Kahl et al [Kahl, 2004] subsequently showed that the GPs derived from RRV and SFV can pseudotype HIV-1-derived lentivirus vectors and that both RRV and SFV GPs considerably expanded the host range of these vectors. Also, such pseudotypes could be efficiently concentrated by ultracentrifugation.…”

Section: Lentiviral Vectors Pseudotyped With the Rrv And Sfv Gps-kangmentioning

confidence: 99%

Altering the Tropism of Lentiviral Vectors through Pseudotyping

Cronin

Zhang²,

Reiser³

2005

CGT

469

338

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The host range of retroviral vectors including lentiviral vectors can be expanded or altered by a process known as pseudotyping. Pseudotyped lentiviral vectors consist of vector particles bearing glycoproteins (GPs) derived from other enveloped viruses. Such particles possess the tropism of the virus from which the GP was derived. For example, to exploit the natural neural tropism of rabies virus, vectors designed to target the central nervous system have been pseudotyped using rabies virusderived GPs. Among the first and still most widely used GPs for pseudotyping lentiviral vectors is the vesicular stomatitis virus GP (VSV-G), due to the very broad tropism and stability of the resulting pseudotypes. Pseudotypes involving VSV-G have become effectively the standard for evaluating the efficiency of other pseudotypes. This review samples a few of the more prominent examples from the ever-expanding list of published lentiviral pseudotypes, noting comparisons made with pseudotypes involving VSV-G in terms of titer, viral particle stability, toxicity, and host-cell specificity. Particular attention is paid to publications of successfully targeting a specific organ or cell types.

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“…To broaden the host range of lentiviral vectors, the latter can be pseudotyped, for example, with the vesicular stomatitis virus glycoprotein (VSV-G) or the gibbon ape leukemia virus (GALV) env, which is provided in trans and imparts a broad tropism; VSV-G or GALV pseudotyped vectors can also be concentrated to produce hightiter supernatants. Stable modifications of the viral envelope have been introduced in order to target a desired cell type or to increase the transduction rate (54)(55)(56)(57).…”

Section: Gene Therapy Vectorsmentioning

confidence: 99%

Gene therapy for severe combined immunodeficiency: are we there yet?

Cavazzana‐Calvo¹,

Fischer²

2007

J. Clin. Invest.

196

110

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Inherited and acquired diseases of the hematopoietic system can be cured by allogeneic hematopoietic stem cell transplantation. This treatment strategy is highly successful when an HLA-matched sibling donor is available, but if not, few therapeutic options exist. Gene-modified, autologous bone marrow transplantation can circumvent the severe immunological complications that occur when a related HLA-mismatched donor is used and thus represents an attractive alternative. In this review, we summarize the advantages and limitations associated with the use of gene therapy to cure SCID. Insertional mutagenesis and technological improvements aimed at increasing the safety of this strategy are also discussed.Nonstandard abbreviations used: AAV, adeno-associated virus; ADA, adenosine deaminase; γc, γ common; GALV, gibbon ape leukemia virus; HIV-1, HIV type 1; HSCT, HSC transplantation; IN, integrase; LMO2, LIM domain only 2; LTR, long-term repeat; MLV, murine leukemia virus; SCID-X1, X-linked SCID; ZFN, zinc finger nuclease; ZFP, zinc finger protein.

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Human Immunodeficiency Virus Type 1-Derived Lentivirus Vectors Pseudotyped with Envelope Glycoproteins Derived from Ross River Virus and Semliki Forest Virus

Cited by 52 publications

References 50 publications

Characterization of hepatitis C virus pseudoparticles by cryo-transmission electron microscopy using functionalized magnetic nanobeads

Characterization of hepatitis C virus pseudoparticles by cryo-transmission electron microscopy using functionalized magnetic nanobeads

Altering the Tropism of Lentiviral Vectors through Pseudotyping

Gene therapy for severe combined immunodeficiency: are we there yet?

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