Human Insulin-Receptor Gene: Partial Sequence and Amplification of Exons by Polymerase Chain Reaction

Abstract

The partial sequence of the human insulin-receptor (hINSR) gene is presented. Using the gene sequence as a guide, we selected pairs of oligonucleotide primers from sites in the introns that flank each exon. These primers allowed each of the 22 exons of the hINSR gene to be amplified in vitro by the polymerase chain reaction. The sequences of the gene and oligonucleotide primers will facilitate studies of genetic variation in the hINSR gene and thereby increase our understanding of the role of this gene in the development of insulin-resistant states and glucose intolerance.

Involvement of Heat Shock Protein 90 in the Degradation of Mutant Insulin Receptors by the Proteasome

“…Expression of Glutathione S-Transferase-Insulin Receptor Cytoplasmic Domain Fusion Protein-The cytoplasmic domain cDNAs of the wild-type and mutant insulin receptors were digested from pGEM3SVHIR expression vectors with BglI (codon 958) and SpeI (nucleotide 13100 of 3Ј intron) (24). The fragments containing the cytoplasmic domain of insulin receptor (IRc) were filled into blunt end by use of T4 DNA polymerase.…”

Leprechaunism (Donohue syndrome): A case bearing novel compound heterozygous mutations in the insulin receptor gene

“…Genomic DNA was isolated from peripheral blood lymphocytes of the patient and his parents, and amplified by PCR as described [7,8].…”

Hyperinsulinemia is associated with altered insulin receptor mRNA splicing in muscle of the spontaneously obese diabetic rhesus monkey.

“…It encodes a transmembrane protein consisting of two extracellular a-subunits and two transmembrane /-subunits (a232)-Insulin binds extracellularly, leading to autophosphorylation and activation of the receptor's tyrosine kinase. Subsequent phosphorylation of endogenous substrates leads to some or all of the actions of insulin (5)(6)(7)(8)(9).…”