2009
DOI: 10.1111/j.1600-6143.2009.02765.x
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Human Islet Isolation for Autologous Transplantation: Comparison of Yield and Function Using SERVA/Nordmark Versus Roche Enzymes

Abstract: Islet autotransplantation (IAT) is used to preserve as much insulin-secretory capacity as possible in patients undergoing total pancreatectomy for painful chronic pancreatitis. The enzyme used to dissociate the pancreas is a critical determinant of islet yield, which is correlated with posttransplant function. Here, we present our experience with IAT procedures to compare islet product data using the new enzyme SERVA/Nordmark (SN group; n = 46) with the standard enzyme Liberase-HI (LH group; n = 40). Total isl… Show more

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Cited by 61 publications
(41 citation statements)
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“…In fact, the highest β-cell mass in lot A was comparable to the one in the Liberase group (N.S.). As others reported (2), our data also showed significantly longer digestion times in the NB1 group. The longer exposure of pancreatic tissues to active enzyme at high temperature (37°C) results in the lower islet viability through the enzymatic toxicity and hypoxia (29, 40).…”
Section: Discussionsupporting
confidence: 91%
See 1 more Smart Citation
“…In fact, the highest β-cell mass in lot A was comparable to the one in the Liberase group (N.S.). As others reported (2), our data also showed significantly longer digestion times in the NB1 group. The longer exposure of pancreatic tissues to active enzyme at high temperature (37°C) results in the lower islet viability through the enzymatic toxicity and hypoxia (29, 40).…”
Section: Discussionsupporting
confidence: 91%
“…Several groups have reported the comparison between Liberase HI and collagenase NB1 blends in terms of the efficiency of islet isolations yield and islet quality (2, 8, 9, 10, 39). Recently Brandhorst et al .…”
Section: Discussionmentioning
confidence: 99%
“…11,12 Now most manufacturers provide purified collagenase in a vial along with a separately packaged non-collagenolytic component. [6][7][8][9][10] Consequently, users are given more strategic opportunities for efficient pancreas digestion than ever before. Our modified digestion protocol utilizes the advantage of the new product.…”
Section: Discussionmentioning
confidence: 99%
“…Currently, most manufacturers provide purified collagenase and non-collagenolytic enzyme in separate vials, [6][7][8][9][10] as opposed to previous products which contained both collagenase and non-collagenolytic enzyme at a predetermined ratio in a vial. 11,12 These new products allow users to digest the pancreas in a more flexible way.…”
Section: Introductionmentioning
confidence: 99%
“…Several advances in islet isolation from diseased pancreases have been made in recent years by various techniques, including altering enzyme combinations in the digestion process (Anazawa et al, 2009;Balamurugan et al, 2011a;Balamurugan et al, 2011c) and other maneuvers (Matsumoto, 2011) such as altering the high density gradient process for purification (Soltani et al, 2011a). There is a clear relationship also to islet yield depending on the severity of the imaging changes of gross pancreatic morphology (Takita et al, 2010) and the severity of the histopathology in both adults (Kobayashi et al, 2011) and children (Kobayashi et al, 2010).…”
Section: Islet Isolation and Infusion Considerationsmentioning
confidence: 99%