Human Kallikrein 6 Expression in Salivary Gland Tumors

Darling, Mark; Jackson-Boeters, Linda; Daley, Tom D.; Diamandis, Eleftherios P.

doi:10.1369/jhc.5a6803.2005

Cited by 21 publications

(15 citation statements)

References 20 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…These results are similar to previous studies that found increased expression of KLK6 in primary pancreatic ductal adenocarcinoma (Ruckert et al, 2008) and salivary gland tumours (Darling et al, 2006), but found no significant association with survival. Kallikreins 6 and 13 are expressed in normal epithelium (Petraki et al, 2001(Petraki et al, , 2003, including the OSE as we have shown here.…”

Section: Discussionsupporting

confidence: 91%

KLK6 and KLK13 predict tumor recurrence in epithelial ovarian carcinoma

et al. 2009

View full text Add to dashboard Cite

BACKGROUND: The human kallikrein-related peptidase family consists of 15 genes. Twelve of these genes are overexpressed in ovarian cancer and may represent potential markers for diagnosis, prognosis, and/or response to treatment. The aim of this study was to determine the prognostic significance of kallikrein-related peptidase 6 (KLK6) and kallikrein-related peptidase 13 (KLK13) in epithelial ovarian cancer by quantifying gene expression levels with tumour pathology and patient survival data. METHODS: Total RNA was isolated from 106 patients diagnosed with primary ovarian cancer, as well as 8 normal ovary controls. Samples were analysed by quantitative real-time PCR for KLK6 and KLK13 expression. Correlation between kallikrein gene expression and clinical characteristics was evaluated with the w 2 -test. Survival analysis was performed using Kaplan -Meier and Cox proportional hazards regression models. RESULTS: Expression levels of both KLK6 and KLK13 mRNA were significantly increased in invasive cancers relative to normal ovaries (P ¼ 0.002 and 0.039 respectively). High KLK6 and KLK13 expression was an indicator of poor prognosis, with patients having a shorter recurrence-free survival (P ¼ 0.002 and 0.027 respectively). High KLK6 expression was also significantly associated with lower overall survival (P ¼ 0.011). When subjected to multivariate analysis, patients with either high KLK6 or KLK13 were 3-and 2.2-fold, respectively, more likely to have a recurrence than patients with low kallikrein expression. CONCLUSION: These data show increased mRNA expression of KLK6 and KLK13 in ovarian cancer compared to normal ovarian tissues. High KLK6 or KLK13 expression in primary ovarian tumours can significantly predict prognosis in terms of recurrence-free survival and overall survival. In all, this study shows KLK6 and KLK13 as potential biomarkers and may be therapeutic targets for treatment of ovarian cancer.

show abstract

Section: Discussionsupporting

confidence: 91%

KLK6 and KLK13 predict tumor recurrence in epithelial ovarian carcinoma

et al. 2009

View full text Add to dashboard Cite

show abstract

“…tumor recurrence, survival rates, and prognosis). We have previously demonstrated the high expression of KLK13, and absence of, or low expression of KLK3, KLK5 and KLK6 in salivary gland tumors (see Table 3 for comparison with results for KLK8) [31][32][33]. As suggested by Paliouras and Diamandis, the kallikreins which are upregulated may be due to a coordinated activation [34], which in salivary gland tumors may be controlled by as yet unrecognised mechanisms, meriting further investigation.…”

Section: Resultsmentioning

confidence: 71%

Human Kallikrein 8 Expression in Salivary Gland Tumors

Darling

Tsai

Jackson-Boeters

et al. 2008

Head and Neck Pathol

View full text Add to dashboard Cite

The human kallikrein 8 protein (KLK8) is expressed in many normal tissues including esophagus, skin, testis, tonsil, kidney, breast, and salivary gland, and is found in biological fluids including breast milk, amniotic fluid, seminal fluid and serum. It has also been shown to be a biomarker and prognostic factor for breast cancer. The aim of this study was to determine whether KLK8 is expressed in salivary gland tissues and salivary gland tumors (both benign and malignant), in order to compare normal with tumor tissues. Pleomorphic adenomas, adenoid cystic carcinomas, polymorphous low grade adenocarcinomas, acinic cell carcinomas, mucoepidermoid carcinomas, and adenocarcinomas NOS of both minor and major salivary glands were examined. The results of this study indicate that most salivary gland tumors show high levels of expression of KLK8.

show abstract

“…6) revealed that 37 peptides result from cleavage before or after Arginine, which is most likely related to the kallikrein family [34][35][36], which has a wide tissue distribution including the salivary glands.…”

Section: Cleavage Sitementioning

confidence: 99%

Analysis of the Human Salivary Peptidome by Differential Peptide Display and LC-MS/MS Overview Sequencing

Yondre¹,

Tammen²,

Hess³

et al. 2008

TOPROTJ

View full text Add to dashboard Cite

Abstract:In recent years interest in the characterization of the human salivary proteome has increased in order to explore its diagnostic potential. Major constituents of human saliva are highly polymorphic proteins that may have biological roles in oral lubrication and protection, e.g. proline-rich proteins (PRPs), statherins, histatins and cystatins. Interestingly, many of theses proteins are rapidly degraded in the oral cavity by host-and bacteria-or viral-derived proteases. Thus, comprehensive analysis of peptides up to 15 kDa (peptidomics) of whole saliva may yield basic information on proteolytic patterns. By employing a LC-ESI-MS/MS approach a total of 107 native peptides from 17 distinct protein precursors was identified from whole saliva. Subsequently the catalog of peptides was used to analyze inter-individual differences in saliva samples from four donors by differential peptide display technology. Genetic polymorphisms were found in peptides from the PRB4M_HUMAN and PROL4_HUMAN precursors. Analysis of the proposed N-and C-termini of the peptides revealed frequent cleavage after Lys or Arg which is characteristic for salivary kallikrein enzymes. Furthermore, we highlight the cleavage motif Gln/Gly in the PRP-C precursor, which suggests a new proteolytic pattern in saliva.

show abstract

Human Kallikrein 6 Expression in Salivary Gland Tumors

Cited by 21 publications

References 20 publications

KLK6 and KLK13 predict tumor recurrence in epithelial ovarian carcinoma

KLK6 and KLK13 predict tumor recurrence in epithelial ovarian carcinoma

Human Kallikrein 8 Expression in Salivary Gland Tumors

Analysis of the Human Salivary Peptidome by Differential Peptide Display and LC-MS/MS Overview Sequencing

Contact Info

Product

Resources

About