Human Lymphoblastoid Cell Line Panels: Novel Tools for Assessing Shared Drug Pathways

Abstract

Comparing the growth-inhibition profiles of drugs (or compounds) of interest with the profiles of drugs with known pathways may assist in drug pathway classification. The method is useful for in vitro assessment of in silico-generated drug pathway predictions and for distinguishing shared versus distinct pathways for compounds of interest. Comparative transcriptomics analysis of human lymphoblastoid cell lines exhibiting 'edge' sensitivities can subsequently be utilized in the search for drug response biomarkers for personalized pharmacotherapy. The limitations and advantages of the method are discussed.

Whole-cell biosensor for label-free detection of GPCR-mediated drug responses in personal cell lines

“…However, LCLs are mainly used as a source of DNA or RNA for genotyping, expression or methylation studies (Docherty et al, 2012;Sie et al, 2009). Functional cellular assays on LCLs have seldom been performed (Li et al, 2014;Morag et al, 2010;Sie et al, 2009) with virtually none for GPCRs. Only Morag and Gurwitz et al studied the influence of a few GPCR antagonists on LCL growth (Morag et al, 2010).…”

“…Functional cellular assays on LCLs have seldom been performed (Li et al, 2014;Morag et al, 2010;Sie et al, 2009) with virtually none for GPCRs. Only Morag and Gurwitz et al studied the influence of a few GPCR antagonists on LCL growth (Morag et al, 2010). In fact, many traditional cellular assays, especially for GPCRs, are incompatible with LCLs as they require labeling and cell or target engineering.…”

Chemo-sensitizing effects of EP4 receptor-induced inactivation of nuclear factor-κB

“…The cells retain most of phenotypic properties of B lymphocytes, including the expression of surface markers CD19, CD20 and production of antibodies. They are therefore considered as a valuable model of primary B lymphocytes (Abbasi et al, 2012;Morag et al, 2010). LCLs were assayed under the same conditions as other cell types.…”

“…These cells are generated from peripheral B lymphocytes of unrelated healthy individuals by Epstein-Barr virus (EBV) transformation. LCLs retain most of the phenotypic properties of B lymphocytes and are therefore considered as a valuable model of primary B lymphocytes (Morag et al, 2010;Sie et al, 2009). As shown in Fig.…”

Functional analysis and molecular characterization of spontaneously outgrown human lymphoblastoid cell lines

“…LCL are a widely used model system to investigate metabolic pathways in vitro because they are a potentially unlimited source of human DNA and they can easily be manipulated. Hence, the immortalized cell lines enable to study disease pathogenesis in molecular and functional analyses by using LCL originated from patients [1][2][3][4][5]. LCL represent also a model system to study the role of EBV in tumorigenesis because the virus is involved in the pathogenesis of several human malignancies including nasopharyngeal carcinoma, Burkitt's lymphoma and Hodgkin's disease [6,7].…”