Human mesenchymal stem cell adhesion and proliferation in response to ceramic chemistry and nanoscale topography

Abstract

Modification of the chemistry and surface topography of nanophase ceramics was used to provide biomaterial formulations designed to direct the adhesion and proliferation of human mesenchymal stem cells (HMSCs). HMSC adhesion was dependent upon both the substrate chemistry and grain size, but not on surface roughness or crystal phase. Specifically, cell adhesion on alumina and hydroxyapatite was significantly reduced on the 50 and 24 nm surfaces, as compared with the 1500 and 200 nm surfaces, but adhesion on titania substrates was independent of grain size. HMSC proliferation was minimal on the 50 and 24 nm substrates of any chemistry tested, and thus significantly lower than the densities observed on either the 1500 or 200 nm surfaces after 3 or more consecutive days of culture. Furthermore, HMSC proliferation was enhanced on the 200 nm substrates, compared with results obtained on the 1500 nm substrates after 7 or more days of culture. HMSC proliferation was independent of both substrate surface roughness and crystal phase. Rat osteoblast and fibroblast adhesion and proliferation exhibited similar trends to that of HMSCs on all substrates tested. These results demonstrated the potential of nanophase ceramic surfaces to modulate functions of HMSCs, which are pertinent to biomedical applications such as implant materials and devices.

Degradable biocomposite of nano calcium- deficient hydroxyapatite-multi(amino acid) copolymer

“…26 The cell adhesion results of this study indicate that MG-63 osteoblast-like cells adhered better to the n-CDHA-MAC composite with 30 wt% n-CDHA than to the MAC and to the tissue culture plate control within the first 4 hours. The superior ability of MG-63 cells to attach to the composite probably reflects the differing material surface features of the composite and the MAC.…”

“…The nanosized calcium-deficient hydroxyapatite in the MAC expose the composite surfaces that had additional surface properties, which improved cell adhesion. 26,27 Cell viability…”

Poly (hydroxyethyl methacrylate-glycidyl methacrylate) films modified with different functional groups: In vitro interactions with platelets and rat stem cells

“…The thickness of the wet films was about 450 μm. The base film (i.e., p(HEMA-GMA)-OH) density was measured to be 1.19 g/cm 3 . The FTIR studies were presented as supplementary data (Supplementary Table 2).…”

“…The properties of hydrogels such as the functional groups, chemical composition, hydrophilic-hydrophobic balance, surface morphology, and presence of adherent proteins are essential for the regulation of cellular interactions. Therefore, the performance of a hydrogel material relies highly upon the properties of the boundaries in tissue engineering applications because the interactions between the hydrogel materials and environments occur mainly on its surfaces [1][2][3][4]. The hydroxyethyl methacrylate (pHEMA) hydrogel possesses good biocompatibility as evidenced by its large scale use in biomedical and biotechnological applications such as contact lenses, drug delivery systems, and soft tissue prosthesis [5][6][7][8][9].…”

Review of biophysical factors affecting osteogenic differentiation of human adult adipose-derived stem cells

“…The effect of mechanical stimulus on cell behavior is affected by intrinsic properties of the cell and properties of the ECM as well as extrinsic forces acting on the cell (Chou et al 2009;Dulgar-Tulloch et al 2009;Jaalouk and Lammerding 2009;McCullen et al 2010b). Even when considering strategies for the rather specific purpose of bone engineering with cells derived from human fat, there are several parameters for influencing the osteogenic differentiation; for instance, media formulation (Lindroos et al 2010;Lund et al 2009), oxygen tension (He et al 2010), harvest location, and sex of donor (Aksu et al 2008) have all been reported to have an effect on the osteogenic potential of ASCs.…”