Human mesenchymal stem cell (hMSC) differentiation towards cardiac cells using a new microbioanalytical method

Sokołowska, Patrycja; Żukowski, Kamil; Lasocka, Iwona; Szulc-Dąbrowska, Lidia; Jastrzębska, Elżbieta

doi:10.1039/c9an02366f

Cited by 9 publications

(6 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…To differentiate the hMSCs, pharmacological agents such as the vascular endothelial growth factor (VEGF) and 5-AZA were utilized in one study by using a microfluidic system, which is capable of the precise regulation of the media flow, its timing, the pattern of the reagent supply, and the incubation period of the cultured cells. The authors have announced promising results using this combined approach as well [ 301 ].…”

Section: Discussionmentioning

confidence: 99%

“…The microenvironment is influenced by protein adhesives (such as laminin, fibronectin, and vitronectin), fibrous structural proteins (such as elastin and collagen), polysaccharides in the form of glycosaminoglycan, and proteoglycan additionally. All of these variables should be precisely taken into account while one is conducting stem cell differentiation experiments [ 301 , 305 ].…”

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Epigenetic Modification Factors and microRNAs Network Associated with Differentiation of Embryonic Stem Cells and Induced Pluripotent Stem Cells toward Cardiomyocytes: A Review

Zare

Salehpour

Khoradmehr

et al. 2023

Life

View full text Add to dashboard Cite

More research is being conducted on myocardial cell treatments utilizing stem cell lines that can develop into cardiomyocytes. All of the forms of cardiac illnesses have shown to be quite amenable to treatments using embryonic (ESCs) and induced pluripotent stem cells (iPSCs). In the present study, we reviewed the differentiation of these cell types into cardiomyocytes from an epigenetic standpoint. We also provided a miRNA network that is devoted to the epigenetic commitment of stem cells toward cardiomyocyte cells and related diseases, such as congenital heart defects, comprehensively. Histone acetylation, methylation, DNA alterations, N6-methyladenosine (m6a) RNA methylation, and cardiac mitochondrial mutations are explored as potential tools for precise stem cell differentiation.

show abstract

Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Epigenetic Modification Factors and microRNAs Network Associated with Differentiation of Embryonic Stem Cells and Induced Pluripotent Stem Cells toward Cardiomyocytes: A Review

Zare

Salehpour

Khoradmehr

et al. 2023

Life

View full text Add to dashboard Cite

show abstract

“…3D culture promotes an environment for better signaling between cells and their neighboring, and causes more realistic physiological, biomechanical, and biochemical properties, and less substrate stiffness than 2D environment, collectively increasing differentiation capability of MSCs into different cell lines. A combination of these conditions alters gene expression of MSCs and thus, activates different signaling pathways ultimately improving efficacy of MSCs differentiation 47 . Pineda et al, investigated myocardial differentiation of embedded mouse embryonic stem cells (M-ESCs) in collagen type I gels (GELs) 48 .…”

Section: Discussionmentioning

confidence: 99%

Improvement of Heart Function After Transplantation of Encapsulated Stem Cells Induced with miR-1/Myocd in Myocardial Infarction Model of Rat

et al. 2021

View full text Add to dashboard Cite

Cardiovascular disease is one of the most common causes of death worldwide. Mesenchymal stem cells (MSCs) are one of the most common sources in cell-based therapies in heart regeneration. There are several methods to differentiate MSCs into cardiac-like cells, such as gene induction. Moreover, using a three-dimensional (3D) culture, such as hydrogels increases efficiency of differentiation. In the current study, mouse adipose-derived MSCs were co-transduced with lentiviruses containing microRNA-1 ( miR-1) and Myocardin ( Myocd). Then, expression of cardiac markers, such as NK2 homeobox 5( Nkx2-5), GATA binding protein 4 ( Gata4), and troponin T type 2 ( Tnnt2) was investigated, at both gene and protein levels in two-dimensional (2D) culture and chitosan/collagen hydrogel (CS/CO) as a 3D culture. Additionally, after induction of myocardial infarction (MI) in rats, a patch containing the encapsulated induced cardiomyocytes (iCM/P) was implanted to MI zone. Subsequently, 30 days after MI induction, echocardiography, immunohistochemistry staining, and histological examination were performed to evaluate cardiac function. The results of quantitative real -time polymerase chain reaction (qRT-PCR) and immunocytochemistry showed that co-induction of miR-1 and Myocd in MSCs followed by 3D culture of transduced cells increased expression of cardiac markers. Besides, results of in vivo study implicated that heart function was improved in MI model of rats in iCM/P-treated group. The results suggested that miR-1/Myocd induction combined with encapsulation of transduced cells in CS/CO hydrogel increased efficiency of MSCs differentiation into iCMs and could improve heart function in MI model of rats after implantation.

show abstract

“…Antibodies suitable for such proteins are usually used for fluorescence imaging [ 99 ]. Table 1 summarizes the studies that have monitored the differentiation process of stem cells over the past three years using immunocytochemistry [ 95 , 98 , 100 , 101 , 102 , 103 , 104 , 105 , 106 , 107 , 108 , 109 , 110 , 111 , 112 , 113 , 114 , 115 , 116 , 117 , 118 , 119 , 120 , 121 , 122 , 123 , 124 , 125 , 126 , 127 , 128 , 129 , 130 , 131 , 132 , 133 , 134 , 135 , 136 ]. ICC is a non-destructive method, different from Western blot.…”

Section: Latest Technological Advances In Monitoring Stem Cell Status...mentioning

confidence: 99%

Recent Advances in Monitoring Stem Cell Status and Differentiation Using Nano-Biosensing Technologies

et al. 2022

View full text Add to dashboard Cite

In regenerative medicine, cell therapies using various stem cells have received attention as an alternative to overcome the limitations of existing therapeutic methods. Clinical applications of stem cells require the identification of characteristics at the single-cell level and continuous monitoring during expansion and differentiation. In this review, we recapitulate the application of various stem cells used in regenerative medicine and the latest technological advances in monitoring the differentiation process of stem cells. Single-cell RNA sequencing capable of profiling the expression of many genes at the single-cell level provides a new opportunity to analyze stem cell heterogeneity and to specify molecular markers related to the branching of differentiation lineages. However, this method is destructive and distorted. In addition, the differentiation process of a particular cell cannot be continuously tracked. Therefore, several spectroscopic methods have been developed to overcome these limitations. In particular, the application of Raman spectroscopy to measure the intrinsic vibration spectrum of molecules has been proposed as a powerful method that enables continuous monitoring of biochemical changes in the process of the differentiation of stem cells. This review provides a comprehensive overview of current analytical methods employed for stem cell engineering and future perspectives of nano-biosensing technologies as a platform for the in situ monitoring of stem cell status and differentiation.

show abstract

Human mesenchymal stem cell (hMSC) differentiation towards cardiac cells using a new microbioanalytical method

Abstract: A microbioanalytical method of stem cell (SC) differentiation towards cardiac cells using a newly developed digitally controlled microdispenser.

Cited by 9 publications

References 42 publications

Epigenetic Modification Factors and microRNAs Network Associated with Differentiation of Embryonic Stem Cells and Induced Pluripotent Stem Cells toward Cardiomyocytes: A Review

Epigenetic Modification Factors and microRNAs Network Associated with Differentiation of Embryonic Stem Cells and Induced Pluripotent Stem Cells toward Cardiomyocytes: A Review

Improvement of Heart Function After Transplantation of Encapsulated Stem Cells Induced with miR-1/Myocd in Myocardial Infarction Model of Rat

Recent Advances in Monitoring Stem Cell Status and Differentiation Using Nano-Biosensing Technologies

Contact Info

Product

Resources

About