Human Mesenchymal Stromal Cell Enhanced Morphological Polarization by Contact Interaction with Polyethylene Terephthalate Nanogratings

Abednatanzi, Sara; Cappelluti, Martino Alfredo; Meucci, Sandro; Jacchetti, Emanuela; Vittorio, Orazio; Parchi, Paolo Domenico; Lisanti, Michele; Pacini, Stefania; Petrini, Mario; Beltram, Fabio; Cecchini, Marco

doi:10.2174/1573413710666140815205825

Cited by 4 publications

(7 citation statements)

References 33 publications

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“…(c) Representative bright-field microscopy images of hMSCs cultured on FLAT, T1 and T2. hMSC mechanotransduction mechanisms [24]. Specifically, we showed that cell and nuclear morphology, and cytoskeletal components are similarly affected by NGs, and that NG ridge sizes of 500 nm and 1 μm were both effective in stimulating cell polarization, without compromising cell viability.…”

Section: Introductionmentioning

confidence: 82%

“…The value for thresholding was set on the condition where osteoinduction was maximal (i.e. in the case of the OM and T2 substrate).The alignment angle was measured with respect to the main NG direction; random reference directions were chosen for flat surfaces [24]. Elongation was calculated as the ratio between MinFeret (namely, the longest distance between any two points along the selection boundary, known as 'maximum caliper') and Feret (namely, the minimum caliper diameter) parameters.…”

Section: Osteogenic Differentiation and Bone Matrix Secretion Evaluationmentioning

confidence: 99%

“…An intriguing alternative to the reviewed chemical approach would be to locally tailor the PET physical properties by introducing nanoor micro-topographies to drive specific cell functions locally that promote osteo-integration. Indeed, it is widely accepted that surface structuring can drive adhesion and spreading [17][18][19], migration [20], and differentiation [21] in many cell types, including fibroblasts [22], epithelial [23], endothelial [24], muscle [25], neuronal [26][27][28][29], bone [30] and stem cells [31].…”

Section: Introductionmentioning

confidence: 99%

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Human mesenchymal stromal cell-enhanced osteogenic differentiation by contact interaction with polyethylene terephthalate nanogratings

et al. 2016

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Among the very large number of polymeric materials that have been proposed in the field of orthopedics, polyethylene terephthalate (PET) is one of the most attractive thanks to its flexibility, thermal resistance, mechanical strength and durability. Several studies have been proposed that interface nano- or micro-structured surfaces with mesenchymal stromal cells (MSCs), demonstrating the potential of this technology for promoting osteogenesis. All these studies were carried out on biomaterials other than PET, which remains almost uninvestigated in terms of cell shaping, alignment and differentiation. Here, we study the effect of PET 350-depth nanogratings (NGs) with a ridge and lateral groove size of 500 nm (T1) or 1 μm (T2), on bone marrow-derived human MSC (hMSC) differentiation in relation to the osteogenic fate. We demonstrate that these substrates, especially T2, can promote the osteogenic phenotype more efficiently than standard flat surfaces and that this effect is more marked if cells are cultured in osteogenic medium than in basal medium. Finally, we show that the shape and disposition of calcium hydroxyapatite granules on the different substrates was influenced by the substrate symmetry, being more elongated and spatially organized on NGs than on flat surfaces.

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Section: Introductionmentioning

confidence: 82%

Section: Osteogenic Differentiation and Bone Matrix Secretion Evaluationmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Human mesenchymal stromal cell-enhanced osteogenic differentiation by contact interaction with polyethylene terephthalate nanogratings

et al. 2016

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“…Topographical obstacles can interfere with FA maturation, forcing adhesion assembly in a preferential direction or hampering adhesion maturation [16,21,22]. In this way, topographical modifications of the substrate can be exploited to either favor or demote selected cell activities [11] including adhesion [23], migration [9,24] and differentiation [25,26]. Altogether, the cellular response to the local surface topography is identified as contact guidance [11].…”

Section: Introductionmentioning

confidence: 99%

Sub-micron lateral topography affects endothelial migration by modulation of focal adhesion dynamics

et al. 2015

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Through the interaction with topographical features, endothelial cells tune their ability to populate target substrates, both in vivo and in vitro. Basal textures interfere with the establishment and maturation of focal adhesions (FAs) thus inducing specific cell-polarization patterns and regulating a plethora of cell activities that govern the overall endothelial function. In this study, we analyze the effect of topographical features on FAs in primary human endothelial cells. Reported data demonstrate a functional link between FA dynamics and cell polarization and spreading on structured substrates presenting variable lateral feature size. Our results reveal that gratings with 2 µm lateral periodicity maximize contact guidance. The effect is linked to the dynamical state of FAs. We argue that these results are readily applicable to the rational design of active surfaces at the interface with the blood stream.

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“…However, the majority of the studies was conducted with bone marrow derived mesenchymal stromal cells (BM-MSCs). Very recently our group demonstrated that nanogratings, alternate lines of sub-micrometer sized ridges and grooves, can effectively polarize BM-MSCs by pure contact interaction (Antonini et al, 2014), and promote enhanced osteo-differentiation with respect to flat surfaces (Antonini et al, 2016). There is indeed a large consensus considering BM-MSCs as the progenitors of the skeletal tissue related cell lineages for their ability to differentiate into adipocytes, osteoblasts and chondroblasts (Keating, 2012).…”

Section: Discussionmentioning

confidence: 99%

Nanotopography Induced Human Bone Marrow Mesangiogenic Progenitor Cells (MPCs) to Mesenchymal Stromal Cells (MSCs) Transition

Abednatanzi

Montali

Jacchetti

et al. 2016

Front. Cell Dev. Biol.

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Mesangiogenic progenitor cells (MPCs) are a very peculiar population of cells present in the human adult bone marrow, only recently discovered and characterized. Owing to their differentiation potential, MPCs can be considered progenitors for mesenchymal stromal cells (MSCs), and for this reason they potentially represent a promising cell population to apply for skeletal tissue regeneration applications. Here, we evaluate the effects of surface nanotopography on MPCs, considering the possibility that this specific physical stimulus alone can trigger MPC differentiation toward the mesenchymal lineage. In particular, we exploit nanogratings to deliver a mechanical, directional stimulus by contact interaction to promote cell morphological polarization and stretching. Following this interaction, we study the MPC-MSC transition by i. analyzing the change in cell morphotype by immunostaining of the key cell-adhesion structures and confocal fluorescence microscopy, and ii. quantifying the expression of cell-phenotype characterizing markers by flow cytometry. We demonstrate that the MPC mesengenic differentiation can be induced by the solely interaction with the NGs, in absence of any other external, chemical stimulus. This aspect is of particular interest in the case of multipotent progenitors as MPCs that, retaining both mesengenic and angiogenic potential, possess a high clinical appeal.

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Human Mesenchymal Stromal Cell Enhanced Morphological Polarization by Contact Interaction with Polyethylene Terephthalate Nanogratings

Cited by 4 publications

References 33 publications

Human mesenchymal stromal cell-enhanced osteogenic differentiation by contact interaction with polyethylene terephthalate nanogratings

Human mesenchymal stromal cell-enhanced osteogenic differentiation by contact interaction with polyethylene terephthalate nanogratings

Sub-micron lateral topography affects endothelial migration by modulation of focal adhesion dynamics

Nanotopography Induced Human Bone Marrow Mesangiogenic Progenitor Cells (MPCs) to Mesenchymal Stromal Cells (MSCs) Transition

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